Results 251 to 260 of about 529,464 (310)

Receptor-affinity chromatography

Nature, 1988
Based upon the specificity and reversibility of receptor–ligand interactions, receptor-affinity chromatography is one of the newest affinity methods for the purification of biomolecules.
P, Bailon, D V, Weber
openaire   +2 more sources

Centrifugal affinity chromatography

Journal of Chromatography A, 1990
A new technique termed centrifugal affinity chromatography (CAC) is presented in this paper. CAC combines a high flow-rate, created by centrifugal force, with the specificity of affinity chromatography. This technique has been used for the purification of human immunoglobulin G.
R J, Slingerland, V, Adámek, W H, Scouten   +2 more
openaire   +2 more sources

Receptor Affinity Chromatography

Annals of the New York Academy of Sciences, 1985
Insulin receptor was purified in high yield from cultured 3T3-L1 mouse adipocytes using the bifunctional ligand N alpha B1-(biotinyl-epsilon-aminocaproyl)insulin in conjunction with avidin-Sepharose CL-4B. This ligand is 100% competent as insulin and 60% competent as biotin, as measured by competitive binding assays.
R A, Kohanski, M D, Lane
openaire   +2 more sources

Shielding Affinity Chromatography

Bio/Technology, 1994
Pulsing your columns with polymers prevents nonspecific binding and allows elution by temperature ...
I Y, Galaev, B, Mattiasson
openaire   +2 more sources

Weak-affinity chromatography

Nature, 1990
Weak-affinity chromatography is a new method using readily reversible biospecific recognition as the basis for chromatographic separations.
Sten Ohlson, David Zopf
openaire   +1 more source

DNA Affinity Chromatography

Molecular Biotechnology, 2001
DNA-affinity chromatography has been used for the purification of DNA-binding proteins that control various cellular processes. There have been improvements in coupling methods and choice of supports over the years. The procedure for coupling 5'-aminoethyl-(dT)18 to silica activated with N-hydroxysuccinimide and a carbodiimide has been described. Also,
P S, Chockalingam, L A, Jurado, H W, Jarrett   +2 more
openaire   +2 more sources

Affinity monolith chromatography

Journal of Separation Science, 2006
AbstractThe combined use of monolithic supports with selective affinity ligands as stationary phases has recently given rise to a new method known as affinity monolith chromatography (AMC). This review will discuss the basic principles behind AMC and examine the types of supports and ligands that have been employed in this method.
Rangan, Mallik, David S, Hage
openaire   +2 more sources

Stress-Free Chromatography: Affinity Chromatography

Current Pharmaceutical Biotechnology, 2009
A number of approaches are available in minimizing aggregation of the final protein products. This chapter describes one such approach, i.e., an attempt to avoid stressful conditions that may eventually lead to protein aggregation. Affinity chromatography uses specific interaction between protein to be purified and ligand attached to the column. Due to
Tsutomu, Arakawa, Yoshiko, Kita, Haruna, Sato, Daisuke, Ejima   +3 more
openaire   +2 more sources

Quantitative affinity chromatography

Journal of Biochemical and Biophysical Methods, 1997
Abstract Quantitative affinity chromatography was introduced (1) at a stage when the preparative technique was well established as a method of solute purification, and has been developed to take additional advantage of the chromatographic matrix used for isolation of the solute on the basis of biospecificity.
openaire   +3 more sources

Dye Affinity Chromatography

Current Protocols in Protein Science, 1995
AbstractDye affinity chromatography is a protein purification procedure based on the high affinity of immobilized dyes for the binding sites on many proteins. It is a rapid, inexpensive, and versatile method that is applicable to the purification of crude cellular extracts. This unit presents protocol for the three types of dye affinity chromatography:
openaire   +2 more sources