Advanced Science, EarlyView.AAA ABSTRACT Protein glycosylation and phosphorylation are critical post‐translational modifications (PTMs) governing nearly all cellular functions, yet their analysis remains challenging due to reliance on costly and unsustainable enrichment materials.
Jiaying Li +9 more
wiley +1 more source
Assessing the Impact of CDR Deamidation and Isomerization on mAb Target Binding Using Affinity-Resolved 2D-SEC-SCX-MS Analysis. [PDF]
J Am Soc Mass SpectromYan Y, Tang S, Wang S, Li N.
europepmc +1 more source
Erasable FAP Targeted Spray‐On Probe for Fluorescence‐Guided Surgery
Advanced Science, EarlyView.We present the first erasable spray‐on probe for fluorescence‐guided surgery. Following topical delivery, FAP‐FLASH550 targets fibroblast activation protein–expressing stromal cells, delineating tumor margins with high tumor‐to‐background ratios. A 365 nm UV light pulse rapidly erases fluorescent signals through triazene photodegradation and subsequent
Zachary Rabinowitz +2 more
wiley +1 more source
Characterization of difficult-to-remove host cell proteins in adeno-associated virus downstream processing. [PDF]
Mol Ther Methods Clin DevLeibiger TM, Min L, Lee KH.
europepmc +1 more source
Advanced Science, EarlyView.A mitochondria‐targeted copper depletion nanoplatform (CYN‐CDA@Alb) was developed to selectively disrupt tumor mitochondria copper, which then reprogrammed the tumor immune microenvironment by depressing PD‐L1 and CD47 expression simultaneously. By doing this, CYN‐CDA@Alb reversed radiotherapy‐induced immune tolerance, showing the potential usage of ...
Zaigang Zhou +10 more
wiley +1 more source
Clostridium beijerinckii displays a soluble [FeFe]-hydrogenase/formate dehydrogenase enzyme complex that links H2 and CO2 metabolism. [PDF]
Biochem JDezzani S +3 more
europepmc +1 more source
MetalCenter‐Dependent Selectivity Divergence in MN4 Single‐Atom Catalysts for Aerobic HMF Oxidation
Advanced Science, EarlyView.Two single‐atom catalysts with M‐N4 configurations (FeN4 and CoN4) were developed for selective HMF oxidation. FeN4 achieves 93.9% selectivity toward FFCA via superoxide anions (O2•−), while CoN4 preferentially produces FDCA through hydroxyl radicals (•OH). DFT calculations reveal that distinct adsorption energies and charge redistribution at the metal
Haoyu Wang +6 more
wiley +1 more source
Structural Basis of the Light-Switchable Interaction between an Azobenzene Side Chain in a Biosynthetic Protein and α-Cyclodextrin. [PDF]
ChemistryOpenEichinger A +4 more
europepmc +1 more source
Advanced Science, EarlyView.A semi‐interpenetrating engineered living material (sIHSELM), fabricated via thermosensitive hydroxybutyl chitosan and covalent protein self‐assembly, demonstrates exceptional mechanical properties and environmental stability. Shielding bacteria from harsh conditions, sIHSELM exhibits remarkable efficacy in ulcerative colitis treatment and pollutants ...
Zixian Bao +8 more
wiley +1 more source
Attempts to Achieve Targeted Covalent Inhibition of Hsp90β. [PDF]
Chem Biol Drug DesD'Amico T +3 more
europepmc +1 more source