Results 221 to 230 of about 1,195,650 (280)
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Affinity chromatography of thrombin
Biochimica et Biophysica Acta (BBA) - Enzymology, 1971Bovine thrombin (EC 3.4.4.13) has been purified by adsorption onto p-chlorobenzylamido-e-aminocaproyl agarose, the ligand having been covalently bound to agarose by the cyanogen bromide technique. Thrombin was eluted from the adsorbant with 1.0 M benzamidine and was recovered by gel filtration through Sephadex G-25 at high ionic strength to prevent ...
A R, Thompson, E W, Davie
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Lectin Affinity Chromatography
2023Glycosylation is a non-template-driven posttranslational modification during which linked-sugars and glycans are added to the nascent polypeptide. Over 70% of the eukaryotic proteome is thought to be glycosylated. It is now known that correct glycosylation is essential for the correct folding, solubility, stability, and immunogenicity of proteins.
Brendan F, O'Connor +2 more
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Receptor-affinity chromatography
Nature, 1988Based upon the specificity and reversibility of receptor–ligand interactions, receptor-affinity chromatography is one of the newest affinity methods for the purification of biomolecules.
P, Bailon, D V, Weber
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Shielding Affinity Chromatography
Bio/Technology, 1994Pulsing your columns with polymers prevents nonspecific binding and allows elution by temperature ...
I Y, Galaev, B, Mattiasson
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The affinity chromatography of transketolase
Biochimica et Biophysica Acta (BBA) - Enzymology, 1978A number of possible affinity adsorbents for transketolase (sedoheptulose-7-phosphate:D-glyceraldehyde-3-phosphateglycolaldehydetransferase, EC 2.2.1.1) were prepared. The behaviour of the enzyme from Candida utilis and from Baker's yeast on columns of these and of Blue Sepharose CL-6B was examined, together with the behaviour of the contaminating ...
T, Wood, S, Fletcher
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Nature, 1990
Weak-affinity chromatography is a new method using readily reversible biospecific recognition as the basis for chromatographic separations.
Sten Ohlson, David Zopf
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Weak-affinity chromatography is a new method using readily reversible biospecific recognition as the basis for chromatographic separations.
Sten Ohlson, David Zopf
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Affinity chromatography of glycosyltransferases
Journal of Chromatography A, 1981This review summarizes the use of biospecific chromatography techniques in the purification of mammalian glycosyltransferases. Ligands that are analogues of donor or acceptor substrates have been linked to cyanogen bromide-activated agarose for use as affinity adsorbents.
J E, Sadler, T A, Beyer, R L, Hill
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Molecular Biotechnology, 2001
DNA-affinity chromatography has been used for the purification of DNA-binding proteins that control various cellular processes. There have been improvements in coupling methods and choice of supports over the years. The procedure for coupling 5'-aminoethyl-(dT)18 to silica activated with N-hydroxysuccinimide and a carbodiimide has been described. Also,
P S, Chockalingam +2 more
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DNA-affinity chromatography has been used for the purification of DNA-binding proteins that control various cellular processes. There have been improvements in coupling methods and choice of supports over the years. The procedure for coupling 5'-aminoethyl-(dT)18 to silica activated with N-hydroxysuccinimide and a carbodiimide has been described. Also,
P S, Chockalingam +2 more
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Affinity chromatography of transaminases
Analytical Biochemistry, 1971Abstract It is shown that glutamic-oxaloacetic transaminase from pig heart can be selectively and reversibly bound to a Sepharose column substituted with N′-alkyl derivatives of pyridoxamine 5′-phosphate. A simple procedure is described which includes formation of apotransaminase, its binding to the substituted Sepharose, and its elution under the ...
R, Collier, G, Kohlhaw
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Affinity chromatography of neoglycoproteins
Bioseparation, 2000Glycoproteins, as a class of biomolecules, exhibit much more heterogeneous structures than non-glycosylated proteins. They present a challenging area of research. Model glycoproteins with well-defined protein and carbohydrate structures are helpful in the search for high-resolution methods for the separation of glycoproteins.
Y, Li +4 more
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