Results 201 to 210 of about 308,818 (265)

Affinity monolith chromatography

Journal of Separation Science, 2006
AbstractThe combined use of monolithic supports with selective affinity ligands as stationary phases has recently given rise to a new method known as affinity monolith chromatography (AMC). This review will discuss the basic principles behind AMC and examine the types of supports and ligands that have been employed in this method.
David Hage
exaly   +3 more sources

Affinity Chromatography And Metal Chelate Affinity Chromatography

C R C Critical Reviews in Analytical Chemistry, 1987
The extremely rapid growth in the field of protein chemistry during the past 50 years has been due in large part to the development of very effective techniques of protein separation and analysis. A number of techniques such as electrophoresis or isoelectric focusing, based on the overall charge and mass of a biomolecule, have been widely applied ...
Alexander J. Fatiadi, L. Anderson
openaire   +1 more source

Lectin Affinity Chromatography

Molecular Biotechnology, 1994
Lectins are glycoproteins or proteins that have a selective affinity for a carbohydrate, or a group of carbohydrates. Many purified lectins are readily available and these maybe immobilized to a variety of chromatography supports.
I, West, O, Goldring
openaire   +5 more sources

Receptor-affinity chromatography

Nature, 1988
Based upon the specificity and reversibility of receptor–ligand interactions, receptor-affinity chromatography is one of the newest affinity methods for the purification of biomolecules.
P, Bailon, D V, Weber
openaire   +2 more sources

The affinity chromatography of transketolase

Biochimica et Biophysica Acta (BBA) - Enzymology, 1978
A number of possible affinity adsorbents for transketolase (sedoheptulose-7-phosphate:D-glyceraldehyde-3-phosphateglycolaldehydetransferase, EC 2.2.1.1) were prepared. The behaviour of the enzyme from Candida utilis and from Baker's yeast on columns of these and of Blue Sepharose CL-6B was examined, together with the behaviour of the contaminating ...
T, Wood, S, Fletcher
openaire   +2 more sources

Lectin Affinity Chromatography

2023
Glycosylation is a non-template-driven posttranslational modification during which linked-sugars and glycans are added to the nascent polypeptide. Over 70% of the eukaryotic proteome is thought to be glycosylated. It is now known that correct glycosylation is essential for the correct folding, solubility, stability, and immunogenicity of proteins.
Brendan F, O'Connor, Donal, Monaghan, Jonathan, Cawley   +2 more
openaire   +2 more sources

Weak-affinity chromatography

Nature, 1990
Weak-affinity chromatography is a new method using readily reversible biospecific recognition as the basis for chromatographic separations.
Sten Ohlson, David Zopf
openaire   +1 more source

Affinity chromatography of thrombin

Biochimica et Biophysica Acta (BBA) - Enzymology, 1971
Bovine thrombin (EC 3.4.4.13) has been purified by adsorption onto p-chlorobenzylamido-e-aminocaproyl agarose, the ligand having been covalently bound to agarose by the cyanogen bromide technique. Thrombin was eluted from the adsorbant with 1.0 M benzamidine and was recovered by gel filtration through Sephadex G-25 at high ionic strength to prevent ...
A R, Thompson, E W, Davie
openaire   +2 more sources

Receptor Affinity Chromatography

Annals of the New York Academy of Sciences, 1985
Insulin receptor was purified in high yield from cultured 3T3-L1 mouse adipocytes using the bifunctional ligand N alpha B1-(biotinyl-epsilon-aminocaproyl)insulin in conjunction with avidin-Sepharose CL-4B. This ligand is 100% competent as insulin and 60% competent as biotin, as measured by competitive binding assays.
R A, Kohanski, M D, Lane
openaire   +2 more sources

Centrifugal affinity chromatography

Journal of Chromatography A, 1990
A new technique termed centrifugal affinity chromatography (CAC) is presented in this paper. CAC combines a high flow-rate, created by centrifugal force, with the specificity of affinity chromatography. This technique has been used for the purification of human immunoglobulin G.
R J, Slingerland, V, Adámek, W H, Scouten   +2 more
openaire   +2 more sources