Results 161 to 170 of about 729,330 (210)

Fractionation of Bovine Sarcoplasmic Proteins by DEAE‐Cellulose Chromatography

Journal of Food Science, 1965
SUMMARY A Tris buffer system (starting buffer, 0.04M Tris phosphate, pH 9.0; limiting buffer, 0.5M Tris H 2 PO 4 , pH 3.6) and a concave gradient elution procedure were developed for the fractionation of beef sarcoplasmic proteins by ...
J. H. RAMPTON, A. F. ANGLEMIER, M. W. MONTGOMERY   +2 more
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The chromatography of nucleic acid preparations on deae-cellulose paper

Journal of Chromatography A, 1966
Abstract Our present results indicate that deoxyribonucleic acid (DNA) preparations of calf thymus origin contain different macromolecular species which can be separated by centrifugal DEAE-cellulose paper pulp (CPP) chromatography. These fractions can be attacked by enzyme action or by heating, and the present paper indicates the relationship ...
P. Charles, L. Ledoux
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Use of DEAE-cellulose in inorganic thin-layer chromatography

Journal of Chromatography A, 1970
RF values for 52 metals obtained on DEAE-cellulose in binary solvent systems were surveyed to evaluate the use of DEAE-cellulose in inorganic thin-layer chromatography. RF values were measured as a function of hydrochloric acid concentration in binary solvent systems consisting of hydrochloric acid and an organic solvent (1:20) such as methanol, n ...
R. Kuroda, N. Yoshikuni, K. Kawabuchi
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Purification of sea urchin sperm bindin by DEAE-cellulose chromatography

Analytical Biochemistry, 1983
A procedure for purifying bindin from sperm of the sea urchin Strongylocentrotus purpuratus is presented in detail. The impure bindin, dissolved in 4 M urea, 50 mM sodium phosphate, pH 6.6, is adsorbed to DEAE-cellulose and eluted wit 4 M urea, 650 mM sodium phosphate, pH 6.6. The purified bindin is not contaminated with tubulin or histone HI.
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Use of DEAE-cellulose for the chromatography of RNA of high molecular weight

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968
Abstract RNA and DNA can be eluted from columns of DEAE-cellulose with high concentrations of triethylammonium acetate in aqueous isoproponal. Columns of this type will resolve 18-S and 28-S RNA from rat liver and will fractionate rapidly labelled RNA from both rat liver and Escherichia coli.
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DEAE-cellulose chromatography of creatine kinase isoenzymes — Effect of pH and serum

Clinical Biochemistry, 1979
DEAE-cellulose chromatography (pH 7.0) of human heart extracts revealed the presence of three creatine kinase isoenzymes. The CK3 (skeletal muscle) isoenzyme was not retained on the column under these conditions. The CK2 (heart) and CK1 (brain) isoenzymes eluted at a conductivity of 5.5 +/- 0.6 m omega-1 and 11.4 +/- 1.2m omega-1, respectively.
P R, Desjardins, S W, Rabkin, H K, Jacobs   +2 more
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Chromatography of soluble proteins from the bovine vitreous body on DEAE-cellulose

Experimental Eye Research, 1962
The soluble proteins of the bovine vitreous body were studied by chromatography on diethylaminoethyl cellulose columns, free electrophoresis, and immunoelectrophoresis. Free electrophoresis revealed at least four protein components, but immunoelectrophoresis showed the presence of a minimum of twelve components, at least three of which are not present ...
U B, LAURENT, T C, LAURENT, A F, HOWE
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Experimental problems in the use of commercially prepared DEAE-celluloses for protein chromatography

Analytical Biochemistry, 1966
Abstract Commercially available DEAE-celluloses have been tested in a highly standardized system and found to vary beyond tolerable limits in their physical and chromatographic properties. A simple test is described which makes it possible to define the general properties of a commercial adsorbent without carrying out a full gradient elution.
S R, Himmelhoch, E A, Peterson
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Protein fractionation IV. Characterization of serum lipoproteins by chromatography on deae-cellulose

Biochimica et Biophysica Acta, 1961
Abstract Chromatography on DEAE-cellulose was performed with sera from patients with polycythemia vera and granulocytic leukemia, who were treated with ionic 32 PO 4 . Four 32 P peaks are consistently found in these chromatograms, following introduction of buffers II, IV, V and VII, numbered in the order of increasing phosphate concentration and ...
M D, PRAGER, I C, ATKINS
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Two forms of striatal tyrosine hydroxylase from DEAE-cellulose chromatography

Brain Research, 1983
Tyrosine hydroxylase (TH) in freshly prepared 45,000 g supernatant from rat striatum was fractionated by DEAE-cellulose chromatography. The elution was made with 2 vols. of buffer (50 mM Tris, pH 7.4; 2 mM dithiothreitol) followed by 4 vols. of a linear NaCl gradient (0 0.3 M) in the same buffer.
P Y, Sze, R F, Alderson, B J, Hedrick
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