Sizing of single-stranded regions in double-stranded DNA by preparative benzoylated DEAE-cellulose chromatography [PDF]
The concentration of caffeine required to elute wholly single-stranded DNA from benzoylated DEAE-cellulose is proportional to the polynucleotide length. The use of benzoylated DEAE-cellulose chromatography for isolating and sizing single-stranded regions
Bernard W Stewart, Murray Norris
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DEAE-Cellulose Chromatography of Kidney Worm Antigens
The Journal of Parasitology, 1963From extracts of one collection of kidney worms, nine components were separated; eight contained antigens, some of which were common to all. From a second collection, four components absorbing strongly at 280 mje (and presumed to be largely protein) and seven carbohydrate components were separated; three of the former and five of the latter contained ...
L A, BAISDEN, F G, TROMBA
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Separation of Heparin into Subtractions by DEAE-Cellulose Chromatography
Thrombosis and Haemostasis, 1979SummaryCommercial porcine heparin can be separated into three distinct subtractions by using DEAE-cellulose chromatography and a stepped salt gradient. Gram quantities of heparin can be fractionated by this technique. All three heparin subtractions can accelerate the inhibition of thrombin by antithrombin III with different efficiency.
R H, Yue, T, Starr, M M, Gertler
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Study of adenovirus antigens fractionated by chromatography on DEAE-cellulose
Virology, 1959Abstract Partially purified preparations of adenovirus types 2 and 5 were fractionated by chromatography on DEAE-cellulose columns. Elution with increasing NaCl concentrations, yielded in succession the antigens previously designated C, B, and A, followed by the infective particle.
H G, KLEMPERER, H G, PEREIRA
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Chromatography of Blood Thrombokinase on ‘DEAE’-Cellulose
Nature, 1960THROMBOKINASE, prepared from bovine plasma, has been obtained in a highly purified state by repeated electrophoretic fractionations1. However, a more efficient method was promised by anion-exchange chromatography, provided that the kinase could be separated from its accompanying impurities by stepwise elution in one day2.
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Fractionation of Calf Thymus DNA by DEAE Cellulose Column Chromatography
Preparative Biochemistry, 1982Commercially available calf thymus DNA could be fractionated into different populations by DEAE-cellulose column chromatography, presumably due to the tertiary folding of the solid fibrous form of DNA which opened up gradually in solution. Depending on the duration in solution or the concentration of the solution, various peaks could be obtained by ...
A F, Wu, T T, Wu
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Chromatography of acidic glycosaminoglycans on DEAE-cellulose
Journal of Chromatography A, 1972Abstract An ion-exchange procedure has been developed for the separation and analysis of milligram amounts of acidic glycosaminoglycans. The polysaccharides were first applied to a column of Sephadex G-50 and eluted with 0.15 M sodium chloride.
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Assay for phosphatidylserine decarboxylase utilizing DEAE-cellulose column chromatography
Analytical Biochemistry, 1989A simple assay for phosphatidylserine decarboxylase is described. Following incubation of a mitochondrial fraction from Saccharomyces cerevisiae with purified, exogenous phosphatidyl[3H]serine, the lipid extract is applied to a small DEAE-cellulose column equilibrated in CHCI3-CH3OH (1:1).
J H, Overmeyer, C J, Waechter
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THE CHROMATOGRAPHY ON DEAE-CELLULOSE OF PITUITARY EXTRACTS FROM SEVERAL SPECIES
Journal of Endocrinology, 1964SUMMARY The distribution, potency and yield of growth hormone (GH), prolactin, thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH) activities and the electrophoretic behaviour on starch gel have been studied in fractions obtained by chromatography on DEAE-cellulose of pituitary extracts ...
A L, WALLACE, K A, FERGUSON
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Fractionation of the virus of foot-and-mouth disease by chromatography on DEAE-cellulose
Biochimica et Biophysica Acta, 1959Abstract Crude suspensions of the virus of foot-and-mouth disease have been separated into two main fractions by chromatography on DEAE-cellulose. One fraction fixes complement with the type specific antiserum, has low infectivity and is identical with the 7-mμ component of the virus.
F, BROWN, B, CARTWRIGHT
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