Results 131 to 140 of about 16,885 (184)

Assay for phosphatidylserine decarboxylase utilizing DEAE-cellulose column chromatography

Analytical Biochemistry, 1989
A simple assay for phosphatidylserine decarboxylase is described. Following incubation of a mitochondrial fraction from Saccharomyces cerevisiae with purified, exogenous phosphatidyl[3H]serine, the lipid extract is applied to a small DEAE-cellulose column equilibrated in CHCI3-CH3OH (1:1).
J H, Overmeyer, C J, Waechter
openaire   +2 more sources

THE CHROMATOGRAPHY ON DEAE-CELLULOSE OF PITUITARY EXTRACTS FROM SEVERAL SPECIES

Journal of Endocrinology, 1964
SUMMARY The distribution, potency and yield of growth hormone (GH), prolactin, thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH) activities and the electrophoretic behaviour on starch gel have been studied in fractions obtained by chromatography on DEAE-cellulose of pituitary extracts from man ...
A L, WALLACE, K A, FERGUSON
openaire   +2 more sources

Fractionation of the virus of foot-and-mouth disease by chromatography on DEAE-cellulose

Biochimica et Biophysica Acta, 1959
Abstract Crude suspensions of the virus of foot-and-mouth disease have been separated into two main fractions by chromatography on DEAE-cellulose. One fraction fixes complement with the type specific antiserum, has low infectivity and is identical with the 7-mμ component of the virus.
F, BROWN, B, CARTWRIGHT
openaire   +2 more sources

Use of DEAE-cellulose in inorganic thin-layer chromatography

Journal of Chromatography A, 1970
RF values for 52 metals obtained on DEAE-cellulose in binary solvent systems were surveyed to evaluate the use of DEAE-cellulose in inorganic thin-layer chromatography. RF values were measured as a function of hydrochloric acid concentration in binary solvent systems consisting of hydrochloric acid and an organic solvent (1:20) such as methanol, n ...
R. Kuroda, N. Yoshikuni, K. Kawabuchi
openaire   +1 more source

The chromatography of nucleic acid preparations on deae-cellulose paper

Journal of Chromatography A, 1966
Abstract Our present results indicate that deoxyribonucleic acid (DNA) preparations of calf thymus origin contain different macromolecular species which can be separated by centrifugal DEAE-cellulose paper pulp (CPP) chromatography. These fractions can be attacked by enzyme action or by heating, and the present paper indicates the relationship ...
P. Charles, L. Ledoux
openaire   +1 more source

Purification of sea urchin sperm bindin by DEAE-cellulose chromatography

Analytical Biochemistry, 1983
A procedure for purifying bindin from sperm of the sea urchin Strongylocentrotus purpuratus is presented in detail. The impure bindin, dissolved in 4 M urea, 50 mM sodium phosphate, pH 6.6, is adsorbed to DEAE-cellulose and eluted wit 4 M urea, 650 mM sodium phosphate, pH 6.6. The purified bindin is not contaminated with tubulin or histone HI.
openaire   +2 more sources

Use of DEAE-cellulose for the chromatography of RNA of high molecular weight

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968
Abstract RNA and DNA can be eluted from columns of DEAE-cellulose with high concentrations of triethylammonium acetate in aqueous isoproponal. Columns of this type will resolve 18-S and 28-S RNA from rat liver and will fractionate rapidly labelled RNA from both rat liver and Escherichia coli.
openaire   +2 more sources

Fractionation of Bovine Sarcoplasmic Proteins by DEAE‐Cellulose Chromatography

Journal of Food Science, 1965
SUMMARY A Tris buffer system (starting buffer, 0.04M Tris phosphate, pH 9.0; limiting buffer, 0.5M Tris H 2 PO 4 , pH 3.6) and a concave gradient elution procedure were developed for the fractionation of beef sarcoplasmic proteins by ...
J. H. RAMPTON, A. F. ANGLEMIER, M. W. MONTGOMERY   +2 more
openaire   +1 more source

DEAE-cellulose chromatography of creatine kinase isoenzymes — Effect of pH and serum

Clinical Biochemistry, 1979
DEAE-cellulose chromatography (pH 7.0) of human heart extracts revealed the presence of three creatine kinase isoenzymes. The CK3 (skeletal muscle) isoenzyme was not retained on the column under these conditions. The CK2 (heart) and CK1 (brain) isoenzymes eluted at a conductivity of 5.5 +/- 0.6 m omega-1 and 11.4 +/- 1.2m omega-1, respectively.
P R, Desjardins, S W, Rabkin, H K, Jacobs   +2 more
openaire   +2 more sources

Two forms of striatal tyrosine hydroxylase from DEAE-cellulose chromatography

Brain Research, 1983
Tyrosine hydroxylase (TH) in freshly prepared 45,000 g supernatant from rat striatum was fractionated by DEAE-cellulose chromatography. The elution was made with 2 vols. of buffer (50 mM Tris, pH 7.4; 2 mM dithiothreitol) followed by 4 vols. of a linear NaCl gradient (0 0.3 M) in the same buffer.
P Y, Sze, R F, Alderson, B J, Hedrick
openaire   +2 more sources