Results 181 to 190 of about 29,610 (215)

Two forms of striatal tyrosine hydroxylase from DEAE-cellulose chromatography

Brain Research, 1983
Tyrosine hydroxylase (TH) in freshly prepared 45,000 g supernatant from rat striatum was fractionated by DEAE-cellulose chromatography. The elution was made with 2 vols. of buffer (50 mM Tris, pH 7.4; 2 mM dithiothreitol) followed by 4 vols. of a linear NaCl gradient (0 0.3 M) in the same buffer.
P Y, Sze, R F, Alderson, B J, Hedrick
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Chromatography of acidic glycosaminoglycans on DEAE-cellulose

Journal of Chromatography A, 1972
Abstract An ion-exchange procedure has been developed for the separation and analysis of milligram amounts of acidic glycosaminoglycans. The polysaccharides were first applied to a column of Sephadex G-50 and eluted with 0.15 M sodium chloride.
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Study of adenovirus antigens fractionated by chromatography on DEAE-cellulose

Virology, 1959
Abstract Partially purified preparations of adenovirus types 2 and 5 were fractionated by chromatography on DEAE-cellulose columns. Elution with increasing NaCl concentrations, yielded in succession the antigens previously designated C, B, and A, followed by the infective particle.
H G, KLEMPERER, H G, PEREIRA
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Chromatography of Blood Thrombokinase on ‘DEAE’-Cellulose

Nature, 1960
THROMBOKINASE, prepared from bovine plasma, has been obtained in a highly purified state by repeated electrophoretic fractionations1. However, a more efficient method was promised by anion-exchange chromatography, provided that the kinase could be separated from its accompanying impurities by stepwise elution in one day2.
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Use of DEAE-cellulose in inorganic thin-layer chromatography

Journal of Chromatography A, 1970
RF values for 52 metals obtained on DEAE-cellulose in binary solvent systems were surveyed to evaluate the use of DEAE-cellulose in inorganic thin-layer chromatography. RF values were measured as a function of hydrochloric acid concentration in binary solvent systems consisting of hydrochloric acid and an organic solvent (1:20) such as methanol, n ...
R. Kuroda, N. Yoshikuni, K. Kawabuchi
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Molybdate Affects Glucocorticoid Receptor Chromatographyon Deae Cellulose

Journal of Receptor Research, 1986
How molybdate affects the chromatographic behavior of the glucocorticoid receptor on DEAE cellulose was investigated. Over the range 0-20 mM, molybdate caused a progressive change in the elution positions of both the transformed and untransformed receptor. At 20 mM molybdate, the transformed receptor eluted from the column before application of the KCl
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Fractionation of Bovine Sarcoplasmic Proteins by DEAE‐Cellulose Chromatography

Journal of Food Science, 1965
SUMMARY A Tris buffer system (starting buffer, 0.04M Tris phosphate, pH 9.0; limiting buffer, 0.5M Tris H 2 PO 4 , pH 3.6) and a concave gradient elution procedure were developed for the fractionation of beef sarcoplasmic proteins by ...
J. H. RAMPTON, A. F. ANGLEMIER, M. W. MONTGOMERY   +2 more
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THE CHROMATOGRAPHY ON DEAE-CELLULOSE OF PITUITARY EXTRACTS FROM SEVERAL SPECIES

Journal of Endocrinology, 1964
SUMMARY The distribution, potency and yield of growth hormone (GH), prolactin, thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH) activities and the electrophoretic behaviour on starch gel have been studied in fractions obtained by chromatography on DEAE-cellulose of pituitary extracts from man ...
A L, WALLACE, K A, FERGUSON
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Purification of sea urchin sperm bindin by DEAE-cellulose chromatography

Analytical Biochemistry, 1983
A procedure for purifying bindin from sperm of the sea urchin Strongylocentrotus purpuratus is presented in detail. The impure bindin, dissolved in 4 M urea, 50 mM sodium phosphate, pH 6.6, is adsorbed to DEAE-cellulose and eluted wit 4 M urea, 650 mM sodium phosphate, pH 6.6. The purified bindin is not contaminated with tubulin or histone HI.
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The chromatography of nucleic acid preparations on deae-cellulose paper

Journal of Chromatography A, 1966
Abstract Our present results indicate that deoxyribonucleic acid (DNA) preparations of calf thymus origin contain different macromolecular species which can be separated by centrifugal DEAE-cellulose paper pulp (CPP) chromatography. These fractions can be attacked by enzyme action or by heating, and the present paper indicates the relationship ...
P. Charles, L. Ledoux
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