Results 91 to 100 of about 2,291,633 (330)

Structural and functional characterization of chitinase from carnivorous plant Drosera adelae

FEBS Open Bio, EarlyView.
A class I chitinase from the carnivorous plant Drosera adelae was expressed and purified using a yeast system, revealing high enzymatic activity. Structural analyses of the catalytic and chitin‐binding domains identified key tyrosine residues involved in substrate binding, offering insights into the enzyme's adaptation for insect digestion.
Kazunari Yoneda, Yuki Naruse, Yusaku Suizu, Tomohiro Araki, Yoshikazu Hoshi, Haruhiko Sakuraba, Junji Hayashi, Toshihisa Ohshima   +7 more
wiley   +1 more source

Synthesis and Diels-Alder Reactions of 1,2-Dimethylene- and 1,2,9,10-Tetramethylene-[2.2]paracyclophane: New Routes to Bridge-Anellated[2.2]Paracyclophanedienes [PDF]

, 1992
The title compounds 8 and 1 have been synthesized in three steps each from 1,2-dibromo[2.2]paracyclophan-1-ene (2) and 1,2,9,10-tetrabromo[2.2]paracyclophane-1,9-diene (4), respectively. Copper-mediated coupling of vinyl bromides 2 and 4 with methyl- and
Bailey, Beletskaya, Blatter, Blatter, Cava, Chan, Dalton, de Meijere, de Meijere, GröUtzmacher, Hopf, Hopf, Jenekhe, Keehn, Kohnke, KöUnig, Pollmann, Posner, Reiser, Sato, Scharf, SchlöUter, Stoddart, Stoddart, Stoddart, VöUgtle, Wagner, Wegner, Wong   +28 more
core   +2 more sources

Electrical pulse stimulation reflecting the episodic nature of real‐life exercise modulates metabolic and secretory profile of primary human myotubes

FEBS Open Bio, EarlyView.
Here, we introduced an intermittent electrical stimulation protocol mimicking the episodic nature of real‐life exercise in vitro by alternating low‐ and high‐frequency stimulation. In comparison with widely used continuous stimulation, it enhanced the rate of glucose and fatty acid oxidation, but not the myokine release.
Klára Gabrišová, Tímea Kurdiová, Daria Barkova, Natália Pálešová, Jana Babulicová, Silvia Tyčiaková, Marta Novotová, Mária Balážová, Miroslav Sabo, Václav Pustka, Jozef Ukropec, Barbara Ukropcová   +11 more
wiley   +1 more source

Influence of various levels of L-asparaginase II purification on the cytotoxicity, DNA level, and apoptosis in Hep-2 cells

مجلة مركز بحوث التقنيات الاحيائية, 2010
The genetic effects of several concentrations of L–Asparaginase II (ASNase II), produced by Proteus vulgaris strain Pv.U92, at various levels of purification (ultrasonication, precipitation, ion-exchange chromatography and gel filtration chromatography)
Hayfa H. Hassani, Rawa'a J. Toma
doaj   +1 more source

Identification of microtubule-associated proteins in the centrosome, spindle, and kinetochore of the early Drosophila embryo. [PDF]

, 1989
We have developed affinity chromatography methods for the isolation of microtubule-associated proteins (MAPs) from soluble cytoplasmic extracts and have used them to analyze the cytoskeleton of the early Drosophila embryo.
Alberts, BM, Field, CM, Kellogg, DR
core  

Endogenous mammalian lectin localized extracellularly in lung elastic fibers. [PDF]

, 1984
An affinity-purified antibody preparation raised against a beta-galactoside-binding lectin from bovine lung was used to localize a similar lectin in rat lung by immunofluorescence and by electron microscopy after on-grid staining visualized with ...
Barondes, SH, Cerra, RF, Haywood-Reid, PL   +2 more
core   +3 more sources

Production of antibacterial compounds by a Steely hybrid polyketide synthase in Dictyostelium

FEBS Open Bio, EarlyView.
By selecting appropriate production species under optimised culture conditions, new chlorinated dibenzofuran compounds, which previously could not be analysed in sufficient quantities, were identified from cellular slime mould fruiting bodies. The strong antibacterial activity exhibited by these compounds suggests a link to the ecological function of ...
Tomoaki R. Yamashita, Toyonobu Usuki, Robert R. Kay, Tamao Saito   +3 more
wiley   +1 more source

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Dataset of gene cloning and gel filtration chromatography of R-est6

Data in Brief, 2016
The data presented in this article are connected to the research article entitled “Expression, purification and biochemical characterization of a family 6 carboxylesterase from Methylococcus capsulatus (bath)” (Soni et al., 2016 [1]).
Surabhi Soni, Annamma A. Odaneth, Arvind M. Lali, Sanjeev K. Chandrayan   +3 more
doaj   +1 more source

Chromium (III) complexes and their relationship to the glucose tolerance factor : a thesis presented in partial fulfillment of the requirements for the degree of Master of Science in Chemistry at Massey University [PDF]

, 1982
Two forms of the dinicotinate complex Cr(nic)2(H2O)3OH were formed which were yellow and blue, respectively. For the yellow form the nicotinic acid ligands were coordinated via the pyridine ring nitrogen atom but this complex was biologically inactive ...
Cooper, Juan Anton
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