Results 21 to 30 of about 3,037,795 (402)

Precise Measurement of the G+C Content of Deoxyribonucleic Acid by High-Performance Liquid Chromatography

open access: yes, 1989
High-performance liquid chromatography is a promising alternative for determining the G+C content of bacterial deoxyribonucleic acid (DNA). The method which we evaluated involves enzymatic degradation of the DNA to nucleosides by P1 nuclease and bovine ...
M. Mesbah, U. Premachandran, W. Whitman
semanticscholar   +1 more source

Column Chromatography

open access: yesDefinitions, 2020
Column chromatography is used extensively in nucleic acid chemistry to purify or characterize products from chemical reactions. This appendix focuses on separations using silica gel or alumina.

semanticscholar   +1 more source

Development of SPE clean-up procedure for acrylamide determination from potato-based products by GC-MS/MS

open access: yesOpen Agriculture, 2020
The aim of this work was to develop an analytical method for determining acrylamide in potato-based products by gas chromatography and tandem mass spectrometry analysis (GC-MS/MS) using solid-phase extraction (SPE) clean-up.
Negoiță Mioara   +3 more
doaj   +1 more source

The Suitability of RNA from Positive SARS-CoV-2 Rapid Antigen Tests for Whole Virus Genome Sequencing and Variant Identification to Maintain Genomic Surveillance

open access: yesDiagnostics, 2023
The COVID-19 pandemic has transformed laboratory management, with a surge in demand for diagnostic tests prompting the adoption of new diagnostic assays and the spread of variant surveillance tools. Rapid antigen tests (RATs) were initially used only for
Annamaria Cucina   +6 more
doaj   +1 more source

Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

open access: yesProceedings of the National Academy of Sciences of the United States of America, 1972
A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described. The method depends upon annealing poly(adenylic acid)-rich mRNA to oligothymidylic acid-cellulose columns and its elution ...
H. Aviv, P. Leder
semanticscholar   +1 more source

Incorporation of automated buffer exchange empowers high-throughput protein and plasmid purification for downstream uses

open access: yesSLAS Technology, 2023
The continued acceleration of time-to-market product development and rising demand for biotherapeutics have hastened the need for higher throughput within the biopharmaceutical industry.
Patrick A. Kates   +5 more
doaj  

Hydrophobic interaction chromatography [PDF]

open access: yes, 2011
Most proteins and large polypeptides have hydrophobic regions at their surface. These hydrophobic ‘patches’ are due to the presence of the side chains of hydrophobic or non-polar amino acids such as phenylalanine, tryptophan, alanine and methionine ...
Cummins, Phil, O'Connor, Brendan
core   +1 more source

Chemical Characterization and Determination of the Antioxidant Properties of Phenolic Compounds in Three Scutellaria sp. Plants Grown in Colombia

open access: yesMolecules, 2023
Plants of the genus Scutellaria (Lamiaceae) have a wide variety of bioactive secondary metabolites with diverse biological properties, e.g., anti-inflammatory, antiallergenic, antioxidant, antiviral, and antitumor activities.
Silvia M. Porras   +5 more
doaj   +1 more source

Purification and analytical characterization of an anti- CD4 monoclonal antibody for human therapy [PDF]

open access: yes, 1994
A purification process for the monclonal anti-CD4 antibody MAX.16H5 was developed on an analytical scale using (NH&SO, precipitation, anion-exchange chromatography on MonoQ or Q-Sepharose, hydrophobic interaction chromatography on phenyl- Sepharose and ...
Emmrich, F.   +8 more
core   +1 more source

Bioassay-guided isolation and identification of antimicrobial compounds from thyme essential oil by means of overpressured layer chromatography, bioautography and GC-MS [PDF]

open access: yes, 2012
A simple method is described for efficient isolation of compounds having an antibacterial effect. Two thyme (Thymus vulgaris) essential oils, obtained from the market, were chosen as prospective materials likely to feature several bioactive components ...
Andrea Böszörményi   +23 more
core   +1 more source

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