Results 81 to 90 of about 3,141,961 (402)

ATG4B is required for mTORC1‐mediated anabolic activity and is associated with clinical outcomes in non‐small cell lung cancer

open access: yesFEBS Open Bio, EarlyView.
The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan   +6 more
wiley   +1 more source

Fuels characterization studies [PDF]

open access: yes, 1980
Current analytical techniques used in the characterization of broadened properties fuels are briefly described. Included are liquid chromatography, gas chromatography, and nuclear magnetic resonance spectroscopy.
Antoine, A. C.   +2 more
core   +1 more source

Fast determination of biogenic amines in beverages by a core-shell particle column [PDF]

open access: yes, 2015
A fast and reliable HPLC method for the determination of 11 biogenic amines in beverages has been performed. After pre-column derivatization with dansyl-chloride a Kinetex C18 core–shell particle column (100 mm × 4.6 mm, 2.6 μm particle size) has been ...
Giuliana Vinci   +3 more
core   +1 more source

Thin-Layer Chromatography.

open access: yesMethods in molecular biology, 2021
G. Hölzl, Peter Dörmann
semanticscholar   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

open access: yesFEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq   +3 more
wiley   +1 more source

Advances and Applications of Hybrid Graphene-Based Materials as Sorbents for Solid Phase Microextraction Techniques

open access: yesMolecules
The advancement of traditional sample preparation techniques has brought about miniaturization systems designed to scale down conventional methods and advocate for environmentally friendly analytical approaches.
Alessandra Timóteo Cardoso   +2 more
doaj   +1 more source

The Techniques of Thin Layer Chromatography and the Study of Carbohydrates Using Thin Layer Chromatography [PDF]

open access: yes, 1977
Refined Kraft pulp is found to contain D (+), Mannose, D (+) Xylose, and Glucose; and not to contain Galactose. Silia gel plates and Kieselguhr plates were used for the separation.
Hippchen, Dennis J.
core   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

open access: yesFEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso   +4 more
wiley   +1 more source

Purification of Cortactin Using Anion Exchange Chromatography [PDF]

open access: yes, 2014
Cortactin is an actin-binding protein that has been shown to be involved in cellular migration and metastases in cancer. Bacterially expressed and purified cortactin protein is often used in in vitro assays to examine cortactin’s role in promoting cell ...
Priester, Jacob
core   +1 more source

A Monomer-to-Trimer Transition of the Human Mitochondrial Transcription Termination Factor (mTERF) Is Associated with a Loss of in Vitro Activity [PDF]

open access: yes, 2004
The human mitochondrial transcription termination factor (mTERF) is a nuclear-encoded 39-kDa protein that recognizes a mtDNA segment within the mitochondrial tRNALeu(UUR) gene immediately adjacent to and downstream of the 16 S rRNA gene. Binding of mTERF
Asin-Cayuela, Jordi   +2 more
core   +1 more source

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