Results 1 to 10 of about 1,546,582 (373)

Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays

eLife, 2022
In vertebrates, condensin I and condensin II cooperate to assemble rod-shaped chromosomes during mitosis. Although the mechanism of action and regulation of condensin I have been studied extensively, our corresponding knowledge of condensin II remains ...
Makoto M Yoshida, Kazuhisa Kinoshita, Yuuki Aizawa, Shoji Tane, Daisuke Yamashita, Keishi Shintomi, Tatsuya Hirano   +6 more
doaj   +1 more source

CRISPR-Cas9 effectors facilitate generation of single-sex litters and sex-specific phenotypes

Nature Communications, 2021
In areas such as animal research and agriculture a single sex is often required in abundance, leading to wasted resources and ethical considerations. Here the authors develop a CRISPR/Cas9 mediated synthetic lethal system that enables the production of ...
Charlotte Douglas, Valdone Maciulyte, Jasmin Zohren, Daniel M. Snell, Shantha K. Mahadevaiah, Obah A. Ojarikre, Peter J. I. Ellis, James M. A. Turner   +7 more
doaj   +1 more source

Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit

eLife, 2022
Condensin I is a pentameric protein complex that plays an essential role in mitotic chromosome assembly in eukaryotic cells. Although it has been shown that condensin I loading is mitosis specific, it remains poorly understood how the robust cell cycle ...
Shoji Tane, Keishi Shintomi, Kazuhisa Kinoshita, Yuko Tsubota, Makoto M Yoshida, Tomoko Nishiyama, Tatsuya Hirano   +6 more
doaj   +1 more source

De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds [PDF]

Science, 2016
Hi-C for mosquito genomes Most genomes sequenced today are determined through the generation of short sequenced bits of DNA that are computationally pieced together like a jigsaw puzzle. This has resulted in the need for funds and additional data to fill
O. Dudchenko, S. S. Batra, Arina D. Omer, Sarah K Nyquist, Marie Hoeger, Neva C. Durand, M. Shamim, Ido Machol, E. Lander, A. P. Aiden, Erez Lieberman Aiden   +10 more
semanticscholar   +1 more source

Construction of stable mouse artificial chromosome from native mouse chromosome 10 for generation of transchromosomic mice

Scientific Reports, 2021
Mammalian artificial chromosomes derived from native chromosomes have been applied to biomedical research and development by generating cell sources and transchromosomic (Tc) animals.
Satoshi Abe, Kazuhisa Honma, Akane Okada, Kanako Kazuki, Hiroshi Tanaka, Takeshi Endo, Kayoko Morimoto, Takashi Moriwaki, Shusei Hamamichi, Yuji Nakayama, Teruhiko Suzuki, Shoko Takehara, Mitsuo Oshimura, Yasuhiro Kazuki   +13 more
doaj   +1 more source

Guiding functions of the C-terminal domain of topoisomerase IIα advance mitotic chromosome assembly

Nature Communications, 2021
Topoisomerase IIα (topo IIα) is critical for mitotic chromatid assembly. Here the authors report a refinement of the mitotic chromatid reconstitution assay and provide novel insights into the C-terminal domain (CTD) of topo IIα.
Keishi Shintomi, Tatsuya Hirano
doaj   +1 more source

Mitotic DNA synthesis in response to replication stress requires the sequential action of DNA polymerases zeta and delta in human cells

Nature Communications, 2023
DNA replication stress can generate under-replicated DNA regions which is fixed by an atypical form of DNA repair synthesis in mitosis (MiDAS). Here the authors show that translesion and replicative DNA polymerases cooperate via the POLD3 subunit to ...
Wei Wu, Szymon A. Barwacz, Rahul Bhowmick, Katrine Lundgaard, Marisa M. Gonçalves Dinis, Malgorzata Clausen, Masato T. Kanemaki, Ying Liu   +7 more
doaj   +1 more source

Different NIPBL requirements of cohesin-STAG1 and cohesin-STAG2

Nature Communications, 2023
NIPBL is considered the cohesin loader. Here, the authors report that a drastic reduction of NIPBL levels reduces chromatin-bound cohesin-STAG2 genome wide while cohesin-STAG1 increases and can still be found at CTCF-bound sites but cannot form loops.
Dácil Alonso-Gil, Ana Cuadrado, Daniel Giménez-Llorente, Miriam Rodríguez-Corsino, Ana Losada   +4 more
doaj   +1 more source

The megabase-scale crossover landscape is largely independent of sequence divergence

Nature Communications, 2022
The frequency of recombination varies along chromosomes and highly correlates with sequence divergence. Here, the authors show that polymorphisms are not a major determinant of the megabase-scale recombination landscape in Arabidopsis, which is rather ...
Qichao Lian, Victor Solier, Birgit Walkemeier, Stéphanie Durand, Bruno Huettel, Korbinian Schneeberger, Raphael Mercier   +6 more
doaj   +1 more source

Full-length human dystrophin on human artificial chromosome compensates for mouse dystrophin deficiency in a Duchenne muscular dystrophy mouse model

Scientific Reports, 2023
Dystrophin maintains membrane integrity as a sarcolemmal protein. Dystrophin mutations lead to Duchenne muscular dystrophy, an X-linked recessive disorder.
Yosuke Hiramuki, Satoshi Abe, Narumi Uno, Kanako Kazuki, Shuta Takata, Hitomaru Miyamoto, Haruka Takayama, Kayoko Morimoto, Shoko Takehara, Mitsuhiko Osaki, Jun Tanihata, Shin’ichi Takeda, Kazuma Tomizuka, Mitsuo Oshimura, Yasuhiro Kazuki   +14 more
doaj   +1 more source