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Chromosome paints from single copies of chromosomes
Chromosome Research, 2004We have used OmniPlex library technology to construct chromosome painting probes from single copies of flow sorted chromosomes. We show that this whole genome amplification technology is particularly efficient at amplifying single copies of chromosomes for the production of paints and that single aberrant chromosomes can be analysed in this way using ...
Susan, Gribble +4 more
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Comparative painting of mammalian chromosomes
Current Opinion in Genetics & Development, 1997Comparative chromosome painting has shown that synteny has been conserved for large segments of the genome in various placental mammals. Advances such as spectral karyotyping and multicolour 'bar coding' lend speed and precision to comparative molecular cytogenetics. Reciprocal chromosome painting and hybridizations with probes such as yeast artificial
J. WIENBERG, STANYON, ROSCOE ROBERT
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Chromosome painting of Amigo wheat
Theoretical and Applied Genetics, 1994Chromosome painting using multicolor fluorescence in situ hybridization showed that, in addition to the T1AL·1RS translocation derived from rye, a segment from chromosome 3Ae#1 of Agropyron elongatum (2n=10x =70), is present in Amigo wheat. The Agropyron chromosome segment is located on the satellite of chromosome 1B and the translocation chromosome is
J, Jiang, B, Friebe, B S, Gill
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Chromosome painting — principles, strategies and scope
Methods in Cell Science, 2001Chromosome Painting is emerging as a powerful tool in the exact localization of different gene sequences of chromosomes at the microscopic level. It is principally based on molecular hybridization in situ with sequence specific probes on chromosomes. Different strategies have been adopted for the preparation of probes, hybridization and visualization ...
A K, Sharma, A, Sharma
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Microdissection and chromosome painting of plant B chromosomes
Methods in Cell Science, 2001Plant chromosome microdissection techniques together with different isolation and amplification methods of microisolated DNA are described. Such isolated DNA was used to 'chromosome paint' B chromosomes of the dicot Brachycome dichromosomatica and the monocot Secale cereale. It is demonstrated that the specific painting of the described chromosomes was
A, Houen, B L, Field, V A, Saunders
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Comparative chromosome painting
2000Chromosome paint probes are complex genomic probes prepared by random PCR amplification of chromosome samples. Most chromosome samples are obtained by sorting a fluid suspension of chromosomes in a dual laser fluorescence-activated cell sorter [1] although microdissection of chromosomes or chromosome regions may also be used [2].
Malcolm A. Ferguson-Smith +3 more
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Chromosome Painting for Plant Biotechnology
2010Fluorescence in situ hybridization (FISH) is an invaluable tool for chromosome analysis and engineering. The ability to visually localize endogenous genes, transposable elements, transgenes, naturally occurring organellar DNA insertions - essentially any unique sequence larger than 2 kb - greatly facilitates progress.
Akio, Kato +7 more
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Chromosome painting in acute monocytic leukemia
Genes, Chromosomes and Cancer, 1993AbstractAn Alu polymerase chain reaction (PCR) probe specific for chromosome II prepared from the somatic cell hybrid JI was used to analyze karyotypes of eight patients with acute monocytic leukemia (AML‐M5). Chromosome painting confirmed the t(9;II) in one patient and a der(I)t(I;6)t(6;II) in another and allowed the identification of a complex ...
D, Cherif +4 more
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Bulked Oligo-FISH for Chromosome Painting and Chromosome Barcoding
2023Recently developed bulked oligo-FISH is a highly versatile method, which is applicable in any plant species with an assembled genome sequence. This technique allows in situ identification of individual chromosomes, large chromosomal rearrangements, comparative karyotype analysis, or even the reconstruction of the three-dimensional organization of the ...
Denisa, Beránková, Eva, Hřibová
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Chromosome painting of radiation-induced micronuclei
International Journal of Radiation Biology, 1996In this report, in situ hybridization with whole chromosome painting probes was used to paint radiation-induced micronuclei (MN) in three lymphoblastoid cells lines to investigate the frequency of radiation-induced MN. The results obtained for four different chromosomes showed that there was a significant deviation of the numbers of signal-positive MN ...
A, Slavotinek +4 more
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