Results 301 to 310 of about 840,795 (338)

Analysis of plant meiotic chromosomes by chromosome painting.

Methods in molecular biology, 2013
Chromosome painting (CP) refers to visualization of large chromosome regions, entire chromosome arms, or entire chromosomes via fluorescence in situ hybridization (FISH). For CP in plants, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the target species or from a closely related species (comparative chromosome painting, CCP)
M. Lysak, T. Mandáková
semanticscholar   +4 more sources

Chromosome painting in plants

2001
The current 'state-of-art' as to chromosome painting in plants is reviewed. We define different situations described as painting so far: i) Genomic in situ hybridisation (GISH) with total genomic DNA to distinguish alien chromosomes on the basis of divergent dispersed repeats, ii) 'Chromosomal in situ suppression' (CISS) hybridisation with chromosome ...
Schubert, I., Fransz, P.F., Fuchs, J., de Jong, J.H.   +3 more
openaire   +4 more sources

Chromosome painting of Amigo wheat

Theoretical and Applied Genetics, 1994
Chromosome painting using multicolor fluorescence in situ hybridization showed that, in addition to the T1AL·1RS translocation derived from rye, a segment from chromosome 3Ae#1 of Agropyron elongatum (2n=10x =70), is present in Amigo wheat. The Agropyron chromosome segment is located on the satellite of chromosome 1B and the translocation chromosome is
Bikram S. Gill, Jiming Jiang, B. Friebe
openaire   +3 more sources

Chromosome paints from single copies of chromosomes

Chromosome Research, 2004
We have used OmniPlex library technology to construct chromosome painting probes from single copies of flow sorted chromosomes. We show that this whole genome amplification technology is particularly efficient at amplifying single copies of chromosomes for the production of paints and that single aberrant chromosomes can be analysed in this way using ...
Elena Prigmore, D C Burford, Bee Ling Ng, Susan M. Gribble, Nigel P. Carter   +4 more
openaire   +3 more sources

Cross-species chromosome painting

Nature Protocols, 2006
Comparative genomics is an important and expanding field of research, and the genome-wide comparison of the chromosome constitution of different species makes a major contribution to this field. Cross-species chromosome painting is a powerful technique for establishing chromosome homology maps, defining the sites of chromosome fusions and fissions ...
Willem, Rens, Beiyuan, Fu, Patricia C M, O'Brien, Malcolm, Ferguson-Smith   +3 more
openaire   +2 more sources

Comparative chromosome painting

2000
Chromosome paint probes are complex genomic probes prepared by random PCR amplification of chromosome samples. Most chromosome samples are obtained by sorting a fluid suspension of chromosomes in a dual laser fluorescence-activated cell sorter [1] although microdissection of chromosomes or chromosome regions may also be used [2].
Malcolm A. Ferguson-Smith, Fengtang Yang, Willem Rens, Patricia C. M. O’Brien   +3 more
openaire   +2 more sources

Chromosome Painting for Plant Biotechnology

2010
Fluorescence in situ hybridization (FISH) is an invaluable tool for chromosome analysis and engineering. The ability to visually localize endogenous genes, transposable elements, transgenes, naturally occurring organellar DNA insertions - essentially any unique sequence larger than 2 kb - greatly facilitates progress.
Seth D. Findley, Tatiana V. Danilova, James A. Birchler, Zhi Gao, Fangpu Han, Patrice S. Albert, Akio Kato, Jonathan C. Lamb   +7 more
openaire   +3 more sources

Chromosome painting in wheat

1997
Chromosome painting is a technique that allows the specific visualization of an entire chromosome in metaphase spreads and in interphase nuclei by in situ hybridization with a mixture of sequences generated from that particular chromosome (Pinkel et al., 1988; Carter, 1994).
Moshe Feldman, Shahal Abbo, Avraham A. Levy, Juan M. Vega   +3 more
openaire   +2 more sources

Biological Dosimetry by Interphase Chromosome Painting

Radiation Research, 1996
Both fluorescence in situ hybridization of metaphase spreads with whole-chromosome probes and premature chromosome condensation in interphase nuclei have been used in the past to estimate the radiation dose to lymphocytes. We combined these techniques to evaluate the feasibility of using painted interphase chromosomes for biodosimetry. Human peripheral
DURANTE, MARCO, K. George, T. C. Yang
openaire   +4 more sources

Cytogenetic analysis by chromosome painting

Cytometry, 1994
AbstractChromosome painting is a term used to describe the direct visualisation using in situ hybridisation of specific chromosomes in metaphase spreads and in interphase nuclei. Chromosome painting, coupled with fluorescence in situ hybridisation (FISH), is now used routinely to enhance the identification of chromosomal rearrangements, the assignment ...
openaire   +3 more sources