Results 81 to 90 of about 74,687 (303)
Production of Shotgun Libraries Using Random Amplification
BioTechniques, 2001 In the following report, thermal cycling coupled with random 10-mers as primers was used to construct randomly amplified shotgun libraries (RASLs). This approach allowed shotgun libraries to be constructed from nanogram quantities of input DNA.
F. Rohwer +4 more
doaj +1 more source
FRUIT, a scar-free system for targeted chromosomal mutagenesis, epitope tagging, and promoter replacement in Escherichia coli and Salmonella enterica. [PDF]
PLoS ONE, 2012 Recombineering is a widely-used approach to delete genes, introduce insertions and point mutations, and introduce epitope tags into bacterial chromosomes. Many recombineering methods have been described, for a wide range of bacterial species.
Anne M Stringer +7 more
doaj +1 more source
Divide and rule: the bacterial chromosome
Trends in Genetics, 2001 Abstract The Keystone Symposium on Bacterial Chromosomes was held in Santa Fe, NM, USA, from 7–13 February, 2001.
openaire +2 more sources
DNA Replication Errors Drive Genome‐Wide Small Inverted Triplication Dynamics
Advanced Science, EarlyView.This study provides insight into the dynamic equilibrium mechanism of a novel structural variant, small inverted triplication (SIT), which is generated by misalignment of the 3’ flap generated under DNA replication stress with palindromic sequence. Alternatively, the end sequence may fold back on itself to form an inverted fragment.
Yi Lei +12 more
wiley +1 more source
Tracing the origin of megaplasmids and secondary chromosomes in bacterial genomes
, 2013 International audienceComponent of bacterial genomes are separated into the stable, relatively slow-evolving and species-specific chromosomes, and the accessory, exchangeable and rapid-evolving plasmids. The evolution of chromosomes is related to that of
Poirion, Olivier +2 more
core +5 more sources
Murine Model System for EBV-related Diseases [PDF]
, 2007 Epstein-Barr Virus (EBV) is involved in several human malignancies via its latent gene products, which interact with cellular proteins and mimic discrete functions of cellular signalling pathways.
Zychlinska, Magdalena, Zychlinska, M.
core
Advanced Science, EarlyView.TaMYB7‐A1 directly activates TaABI5 to enhance ABA signaling and regulate ABA‐GA homeostasis, enforcing seed dormancy. Its superior allele, derived from wild einkorn introgression, harbors a MITE insertion that elevates expression and two amino acid substitutions that enhance transcriptional activity, collectively generating graded PHS resistance for ...
Hao Wang +18 more
wiley +1 more source
, 2011 Copyright information:Taken from "Quantitative analysis of mutation and selection pressures on base composition skews in bacterial chromosomes"http://www.biomedcentral.com/1471-2164/8/286BMC Genomics 2007;8():286-286.Published online 21 Aug 2007PMCID ...
Carton W Chen (67022), Chi Chen (67021)
core +1 more source
Engineering an Optogenetic pH‐Modulator in Bacteria
Advanced Science, EarlyView.ABSTRACT Cells in many naturally occurring organisms routinely cooperate to control their extracellular pH in a dynamic and reversible manner, but this capability has been underexplored in synthetic biology. Here, we sought to engineer a microbial system that switches between two states —high and low extracellular pH— with minimal human intervention ...
Jenevieve Kuang +7 more
wiley +1 more source
BioTechniques, 2001 Simple sequence repeats (SSRs) were isolated from pearl millet bacterial artificial clones (BACs) without any subcloning steps. SSR sequences were targeted using 3′ end anchored SSR primers. Flanking sequences were isolated by suppression PCR.
X. Qi +5 more
doaj +1 more source