Analysis of the SOS response of
BMC Genomics, 2012 Background The SOS response is a well-known regulatory network present in most bacteria and aimed at addressing DNA damage. It has also been linked extensively to stress-induced mutagenesis, virulence and the emergence and dissemination of antibiotic ...
Sanchez-Alberola Neus +3 more
doaj +1 more source
, 2018 Using a bead-spring model of bacterial DNA polymers of {\em C. crescentus} and {\em E. coli} we show that just $33$ and $38$ effective cross-links at special positions along the chain contour of the DNA can lead to the large-scale organization of the DNA
Agarwal, Tejal +3 more
core +1 more source
Single-molecule real-time sequencing combined with optical mapping yields completely finished fungal genome [PDF]
, 2015 Next-generation sequencing (NGS) technologies have increased the scalability, speed, and resolution of genomic sequencing and, thus, have revolutionized genomic studies.
Datema, Erwin +6 more
core +2 more sources
Embedded CRISPRi Enhances Gene‐Silencing Efficiency in Drosophila
Advanced Science, EarlyView.Current CRISPR interference (CRISPRi) technology in Drosophila has limited efficiency. This study introduces the emCRISPRi platform, which significantly enhances transcriptional silencing efficacy by embedding inhibitory domains within the dCas9 architecture.
Pengchong Fu +7 more
wiley +1 more source
Editorial for Special Issue “Bacterial Toxin-Antitoxin Systems”
MicroorganismsToxin antitoxin systems (TAS) are widely distributed in bacterial chromosomes as well as on mobile genetic elements [...]
Muhammad Kamruzzaman
doaj +1 more source
Production of Shotgun Libraries Using Random Amplification
BioTechniques, 2001 In the following report, thermal cycling coupled with random 10-mers as primers was used to construct randomly amplified shotgun libraries (RASLs). This approach allowed shotgun libraries to be constructed from nanogram quantities of input DNA.
F. Rohwer +4 more
doaj +1 more source
FRUIT, a scar-free system for targeted chromosomal mutagenesis, epitope tagging, and promoter replacement in Escherichia coli and Salmonella enterica. [PDF]
PLoS ONE, 2012 Recombineering is a widely-used approach to delete genes, introduce insertions and point mutations, and introduce epitope tags into bacterial chromosomes. Many recombineering methods have been described, for a wide range of bacterial species.
Anne M Stringer +7 more
doaj +1 more source
The genetics of symbiotic nitrogen fixation: comparative genomics of 14 Rhizobia Strains by resolution of protein clusters. [PDF]
, 2017 The symbiotic relationship between legumes and nitrogen fixing bacteria is critical for agriculture, as it may have profound impacts on lowering costs for farmers, on land sustainability, on soil quality, and on mitigation of greenhouse gas emissions ...
BARRERO, R. +6 more
core +1 more source
Molecular basis for SMC rod formation and its dissolution upon DNA binding. [PDF]
, 2015 SMC condensin complexes are central modulators of chromosome superstructure in all branches of life. Their SMC subunits form a long intramolecular coiled coil, which connects a constitutive "hinge" dimerization domain with an ATP-regulated "head ...
Alipour +57 more
core +6 more sources
Advanced Science, EarlyView.This study employs sc‐RNA sequencing, genetics, and phenotyping to systematically map the cell‐type‐specific immune responses triggered by flg22. It reveals FLS2‐dependent transcriptional reprogramming in epidermal and mesophyll cells, and uncovers crosstalk between immune and hypoxia signaling pathways.
Yaping Zhou +17 more
wiley +1 more source