Results 81 to 90 of about 259,540 (309)

A simple filtration technique for obtaining purified human chromosomes in suspension

BioTechniques, 2014
Here we present a simple method for cleaning polyamine human mitotic chromosomes in solution. This was achieved by filtering intact (unburst) nuclei along with both large and small cytoplasmic debris through a series of different pore sized filters. Pure
Mohammed Yusuf, Neha Parmar, Gurdeep K. Bhella, Ian K Robinson   +3 more
doaj   +1 more source

Chromosome Centromeres: Structural and Analytical Investigations with High Resolution Scanning Electron Microscopy in Combination with Focused Ion Beam Milling [PDF]

, 2009
Whole mount mitotic metaphase chromosomes of different plants and animals were investigated with high resolution field emission scanning electron microscopy (FESEM) to study the ultrastructural organization of centromeres, including metacentric ...
Schroeder-Reiter, E., Wanner, Gerhard
core   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Human Chromosomes

, 2012
An interactive e-learning resource to explore Human Chromosomes and the consequences of changes in chromosome number to health and development.
Richard Flint
core  

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Malignancy-Associated Regions of Transcriptional Activation: Gene Expression Profiling Identifies Common Chromosomal Regions of a Recurrent Transcriptional Activation in Human Prostate, Breast, Ovarian, and Colon Cancers

Neoplasia: An International Journal for Oncology Research, 2003
Despite remarkable advances in our understanding of a genetic basis of cancer, the precise molecular definition of the phenotypically relevant genetic features associated with human epithelial malignancies remains a significant and highly relevant ...
Gennadi V. Glinsky, Anja Krones-Herzig, Anna B. Glinskii   +2 more
doaj   +1 more source

Functional human artificial chromosomes are generated and stably maintained in human embryonic stem cells.

, 2011
We present a novel and efficient non-integrating gene expression system in human embryonic stem cells (hESc) utilizing human artificial chromosomes (HAC), which behave as autonomous endogenous host chromosomes and segregate correctly during cell division.
Moralli, D., Yusuf, M., Blundell, MP, William James, Volpi, Emanuela, Thrasher, A.J., Cowley, S, Moralli, D, Cowley, S., Suhail Khoja, Yusuf, M, Volpi, E., Zoia L. Monaco, Mukherjee, S, Daniela Moralli, Khoja, S, Thrasher, AJ, David Y.L. Chan, Mohammed Yusuf, Khoja, S., Mohammad A. Mandegar, Michael P. Blundell, Blundell, M.P., Sayandip Mukherjee, Mandegar, M.A., Chan, DY, Monaco, ZL, James, W, Volpi, EV, James, W., Emanuela V. Volpi, Sally Cowley, Monaco, Z.L., Adrian J. Thrasher, Mukherjee, S., Mandegar, MA, Chan, D.Y.L.   +36 more
core   +1 more source

Mitotic chromosomes are compacted laterally by KIF4 and condensin and axially by topoisomerase IIα [PDF]

, 2012
© 2012 Samejima et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication dateMitotic chromosome formation involves a relatively minor ...
Cooke, C.A., Lydia C. Green, Itaru Samejima, Flavia de Lima Alves, Farr, CJ, Samejima, I., Earnshaw, W.C., Samejima, K., Vagnarelli, P., Kelly, D.A., Kerr, A., David A. Kelly, Hudson, DF, Hudson, D.F., Carol A. Cooke, Kerr, A, Vargiu, G., Cooke, CA, Giulia Vargiu, Hiromi Ogawa, Green, LC, Alves, FDL, Damien F. Hudson, Ogawa, H, de Lima Alves, F, Ogawa, H., Alves, F.L., Green, L.C., Vagnarelli, P, Kumiko Samejima, Ohta, S, Alastair Kerr, Christine J. Farr, Shinya Ohta, William C. Earnshaw, Vargiu, G, Ohta, S., Farr, C.J., Paola Vagnarelli, Juri Rappsilber, Rappsilber, J., Samejima, I, Kelly, DA, Rappsilber, J, Samejima, K, Earnshaw, WC   +45 more
core   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Chromosome painting in three-toed sloths: a cytogenetic signature and ancestral karyotype for Xenarthra

BMC Evolutionary Biology, 2012
Background Xenarthra (sloths, armadillos and anteaters) represent one of four currently recognized Eutherian mammal supraorders. Some phylogenomic studies point to the possibility of Xenarthra being at the base of the Eutherian tree, together or not with
Azevedo Nathália F, Svartman Marta, Manchester Andrea, de Moraes-Barros Nádia, Stanyon Roscoe, Vianna-Morgante Angela M   +5 more
doaj   +1 more source