Results 171 to 180 of about 49,239 (220)
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A conjugate of trypsin and chymotrypsin
Applied Biochemistry and Biotechnology, 1987A heteroenzyme conjugate retaining activities of two component enzymes from trypsin and chymotrypsin was prepared using N-succinimidyl pyridyl dithiopropionate as crosslinking reagent. The conjugate bound to both trypsin and chymotrypsin affinity columns. Trypsin and chymotrypsin were linked in the ratio of 1:1 on mol basis. The conjugate, when treated
Y S, Rajput, M N, Gupta
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Biochemistry, 1994
We have examined the reaction of N-(benzyloxycarbonyl)-L-alanyl-L-glycyl-L-phenylalanyl chloromethyl ketone (ZAGFCMK) with chymotrypsin (Cht) and have found that, in addition to irreversible alkylation of the enzyme, some of the corresponding hydroxymethyl ketone is produced. For each molecule of hydroxy ketone formed, 3.6 molecules of chymotrypsin are
M, Prorok +3 more
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We have examined the reaction of N-(benzyloxycarbonyl)-L-alanyl-L-glycyl-L-phenylalanyl chloromethyl ketone (ZAGFCMK) with chymotrypsin (Cht) and have found that, in addition to irreversible alkylation of the enzyme, some of the corresponding hydroxymethyl ketone is produced. For each molecule of hydroxy ketone formed, 3.6 molecules of chymotrypsin are
M, Prorok +3 more
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THE CHEMICAL MODIFICATION OF CHYMOTRYPSIN
Canadian Journal of Biochemistry, 1964Chemical modification of chymotrypsin has led to the identification of several amino acid side-chains which are probably constituents of the active site of the enzyme. A single seryl and a single histidyl residue appear to cooperate in catalyzing the bond-breaking process while one or more tryptophanyl residues may be involved in the specific binding ...
G H, DIXON, H, SCHACHTER
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Adhesive Otitis and Chymotrypsin
Acta Oto-Laryngologica, 1964The chemistry, biochemistry and previous clinical experiences with chymotrypsin is briefly mentioned. This preliminary report states the principles of treatment and early results in a number of patients with chronic secretory otitis media.
N H, BUCH, H K, KRISTENSEN
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Studies of the activity of chymotrypsin
Philosophical Transactions of the Royal Society of London. B, Biological Sciences, 1970Abstract Physical and chemical studies of chymotrypsin in solution and kinetic measurements of individual steps in reactions catalysed by this hydrolytic enzyme are reported. The solution studies also provide a basis for correlation of individual reaction steps with structural information obtained by Drs Blow and Hartley and discussed
G P, Hess, J, McConn, E, Ku, G, McConkey
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A Smart Bioconjugate of Chymotrypsin
Biomacromolecules, 2003alpha-Chymotrypsin was immobilized on Eudragit S-100 via covalent coupling with 93% retention of proteolytic activity. The conjugate behaved as a smart biocatalyst and functioned as a pH-dependent reversibly soluble-insoluble biocatalyst. The pH optimum of chymotrypsin broadened on immobilization, and the immobilized preparation showed better stability
Shweta, Sharma +4 more
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On the inhibition of chymotrypsin by ovomucoid
Archives of Biochemistry and Biophysics, 1960Abstract A study has been made of the inhibition of chymotrypsin by ovomucoid. Marked inhibition is obtained only at high ovomucoid to chymotrypsin ratios. Electrophoretic and ultracentrifugal experiments show that this inhibition is accompanied by complex formation between the two proteins.
L, WEIL, S N, TIMASHEFF
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Coaggregate of trypsin and chymotrypsin
Biotechnology and Bioengineering, 1988AbstractThe method of chemical aggregation of enzymes has the advantage of yielding an immobilized enzyme preparation wherein reactor volume can be significantly reduced because of the absence of an inert carrier. A coaggregate of trypsin and chymotrypsin formed by extensive cross‐linking with glutaraldehyde is described. A significant property of this
Y S, Rajput, M N, Gupta
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Inactivation of kinins by chymotrypsin
Biochemical Pharmacology, 1976Abstract The inactivation rate of synthetic bradykinin (BK) by different samples of crystallized α-chymotrypsin at pH 7.0, 30° and a 1:10 enzyme/substrate molar ratio was reduced in the presence of 1,10-phenanthroline. Selective inaetivation of the samples by preincubation with l -(1-tosylamido-2-phenyl) ethyl-chloromethylketone (TPCK) in 1:10 ...
Sampaio, CAM +4 more
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ANAPHYLACTIC REACTION TO CHYMOTRYPSIN
Journal of the American Medical Association, 1960Proteolytic enzyme therapy has been employed in various diseases in which its anti-inflammatory and fibrinolytic effects have been of value. Among such agents, aqueous chymotrypsin (Chymar) has been described as relatively free of undesirable side-effects.
D, LIEBOWITZ, H, RITTER
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