Results 11 to 20 of about 36,426 (210)

A Versatile Optical Clearing Protocol for Deep Tissue Imaging of Fluorescent Proteins in Arabidopsis thaliana. [PDF]

PLoS ONE, 2016
Confocal microscopy is widely used to visualize gene expression patterns and developmental processes in plants. However, the imaging of plant tissue can be challenging due to its opacity, which often makes previous immersion in a clearing agent necessary.
Thomas J Musielak, Daniel Slane, Christian Liebig, Martin Bayer   +3 more
doaj   +1 more source

Ex-vivo confocal Raman microspectroscopy of porcine skin with 633/785-NM laser excitation and optical clearing with glycerol/water/DMSO solution

Journal of Innovative Optical Health Sciences, 2021
Confocal Raman microspectroscopy (CRM) with 633- and 785-nm excitation wavelengths combined with optical clearing (OC) technique was used for ex-vivo study of porcine skin in the Raman fingerprint region.
Ali Jaafar, Malik H. Mahmood, Roman Holomb, László Himics, Tamás Váczi, Anton Y. Sdobnov, Valery V. Tuchin, Miklós Veres   +7 more
doaj   +1 more source

Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect.

PLoS ONE, 2021
The use of clearing agents has provided new insights in various fields of medical research (developmental biology, neurology) by enabling examination of tissue architecture in 3D.
R C M Vulders, R C van Hoogenhuizen, E van der Giessen, P J van der Zaag   +3 more
doaj   +1 more source

“Click-to-Clear”: A Strategy to Minimize Radioactivity from the Blood Pool Utilizing Staudinger Ligation

Pharmaceutics, 2023
The availability of several bioorthogonal reactions that can proceed selectively and efficiently under physiologically relevant conditions has garnered the interest of biochemists and organic chemists alike. Bioorthogonal cleavage reactions represent the
Nisarg Soni, Swarbhanu Sarkar, Abhinav Bhise, Yeong Su Ha, Wonchoul Park, A-Ram Yu, Virendra Kumar, Jeong Eun Lim, Young-Ran Yoon, Jeongsoo Yoo   +9 more
doaj   +1 more source

HyClear: A Novel Tissue Clearing Solution for One-Step Clearing of Microtissues

Cells, 2022
3-D cell cultures are being increasingly used as in vitro models are capable of better mimicry of in vivo tissues, particularly in drug screenings where mass transfer limitations can affect the cancer biology and response to drugs.
S. Soroush Nasseri, Erika M. J. Siren, Jayachandran N. Kizhakkedathu, Karen Cheung   +3 more
doaj   +1 more source

Oil as an Alternative Clearing Agent to Xylene in Histological Staining Process: A Systematic Review [PDF]

The Egyptian Journal of Histology
Clearing in tissue processing removes alcohol and allows the tissue to be infiltrated with paraffin wax. It renders the tissue transparent, making it easier to be examined under the microscope.
Earl Adriane Cano, Adrian Carlo Sandoval, Nyll Jocas Santos, Shanley Daniella Mae Satrain, Keane Kasey Sulita, Jennifer Tan, Alyssa Anne Tolentino, Teschia Lian Tugonon   +7 more
doaj   +1 more source

Efficacy of “groundnut oil” and “coconut oil” as a substitute for “xylene” in clearing tissues samples – A comparative study

SRM Journal of Research in Dental Sciences, 2019
Background: Since yore, “xylene” has always been the nonpareil clearing medium known to be employed in tissue processing and staining. However, the toxic effects of “xylene” range from mild irritation to its severe form as a carcinogenic agent.
Priyadharshini Saravanakumar, R Bharanidharan, Ramya Ramadoss, Aravind, A Ramesh Kumar   +4 more
doaj   +1 more source

A novel strategy for development of recombinant antitoxin therapeutics tested in a mouse botulism model.

PLoS ONE, 2012
Antitoxins are needed that can be produced economically with improved safety and shelf life compared to conventional antisera-based therapeutics. Here we report a practical strategy for development of simple antitoxin therapeutics with substantial ...
Jean Mukherjee, Jacqueline M Tremblay, Clinton E Leysath, Kwasi Ofori, Karen Baldwin, Xiaochuan Feng, Daniela Bedenice, Robert P Webb, Patrick M Wright, Leonard A Smith, Saul Tzipori, Charles B Shoemaker   +11 more
doaj   +1 more source

Kerosene as an alternative to xylene in histopathological tissue processing and staining: An experimental study

Journal of Pharmacy and Bioallied Sciences, 2019
Background: Conventional tissue processing is as old as 100 years and still remains the gold standard. Tissue processing involves many steps, of which one of the important steps is clearing. Xylene is one of the common clearing agents used in laboratory,
Janardhanam Dineshshankar, Manikandan Saranya, Periyasamy Tamilthangam, Jeyaraman Swathiraman, Kumaravel Shanmathee, Ravichandran Preethi   +5 more
doaj   +1 more source

Evaluation of the efficacy of hematoxylin and eosin stain when xylene is completely replaced by turpentine or kerosene oil – A comparative study for oral tissues

Indian Journal of Pathology and Microbiology, 2023
Background: Microscopic examination of cells and tissues requires the preparation of very thin and good-quality sections mounted on glass slides and appropriately stained to demonstrate normal and abnormal structures.
Priyanka Singh, Aparna Dave, Manpreet Arora, Pulin Saluja Madan, Radhika Rai   +4 more
doaj   +1 more source