snRNA 3' End Processing by a CPSF73-Containing Complex Essential for Development in Arabidopsis. [PDF]
PLoS Biology, 2016 Uridine-rich small nuclear RNAs (snRNAs) are the basal components of the spliceosome and play essential roles in splicing. The biogenesis of the majority of snRNAs involves 3' end endonucleolytic cleavage of the nascent transcript from the elongating DNA-
Yunfeng Liu +5 more
doaj +1 more source
PABPN1 Couples the Polyadenylation and Translation of Maternal Transcripts to Mouse Oocyte Meiotic Maturation. [PDF]
Adv Sci (Weinh)This study reveals that cytoplasmic PABPN1 is essential for mouse oocyte meiotic maturation by coordinating polyadenylation, translation, and degradation of maternal mRNAs. Pabpn1 knockout disrupts CDK1 activation, spindle formation, and chromosome alignment by impairing maturation‐promoting factor (MPF) regulation and BTG4‐mediated deadenylation ...
Dai XX +9 more
europepmc +2 more sources
The 68 kDa subunit of mammalian cleavage factor I interacts with the U7 small nuclear ribonucleoprotein and participates in 3′-end processing of animal histone mRNAs [PDF]
, 2010 Metazoan replication-dependent histone pre-mRNAs undergo a unique 3′-cleavage reaction which does not result in mRNA polyadenylation. Although the cleavage site is defined by histone-specific factors (hairpin binding protein, a 100-kDa zinc-finger ...
Azzouz, Teldja N. +6 more
core +2 more sources
Uridylation and adenylation of RNAs. [PDF]
, 2015 The posttranscriptional addition of nontemplated nucleotides to the 3' ends of RNA molecules can have a significant impact on their stability and biological function.
Chen, XueMei +3 more
core +2 more sources
Viruses, 2021 Influenza A viruses (IAV) modulate host antiviral responses to promote growth and pathogenicity. Here, we examined the multifunctional IAV nonstructural protein 1 (NS1) of influenza A virus to better understand factors that contribute to viral ...
Meng-Ting Huang +7 more
doaj +1 more source
Scientific Reports, 2017 Pre-mRNA alternative splicing and alternative polyadenylation have been implicated to play important roles during eukaryotic gene expression. However, much remains unknown regarding the regulatory mechanisms and the interactions of these two processes in
Qingshun Q. Li +3 more
doaj +1 more source
Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification [PDF]
, 2020 Understanding genome organization and gene regulation requires insight into RNA transcription, processing and modification. We adapted nanopore direct RNA sequencing to examine RNA from a wild-type accession of the model plant Arabidopsis thaliana and a ...
Barton, Geoffrey J. +8 more
core +3 more sources
Archaeal Tuc1/Ncs6 homolog required for wobble uridine tRNA thiolation is associated with ubiquitin-proteasome, translation, and RNA processing system homologs. [PDF]
PLoS ONE, 2014 While cytoplasmic tRNA 2-thiolation protein 1 (Tuc1/Ncs6) and ubiquitin-related modifier-1 (Urm1) are important in the 2-thiolation of 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) at wobble uridines of tRNAs in eukaryotes, the biocatalytic roles and ...
Nikita E Chavarria +11 more
doaj +1 more source
RNA-binding protein CPEB1 remodels host and viral RNA landscapes. [PDF]
, 2016 Host and virus interactions occurring at the post-transcriptional level are critical for infection but remain poorly understood. Here, we performed comprehensive transcriptome-wide analyses revealing that human cytomegalovirus (HCMV) infection results in
Aigner, Stefan +17 more
core +2 more sources
Small RNA deep sequencing reveals role for Arabidopsis thaliana RNA-dependent RNA polymerases in viral siRNA biogenesis. [PDF]
PLoS ONE, 2009 RNA silencing functions as an important antiviral defense mechanism in a broad range of eukaryotes. In plants, biogenesis of several classes of endogenous small interfering RNAs (siRNAs) requires RNA-dependent RNA Polymerase (RDR) activities.
Xiaopeng Qi +2 more
doaj +1 more source