Results 161 to 170 of about 746,964 (209)
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Somatic Cell Cloning without Micromanipulators
Cloning, 2001Until now, micromanipulators have been regarded as indispensable for somatic cell nuclear transfer. This paper describes an improved zona-free nuclear transfer procedure with manual bisection of oocytes, selection of cytoplasts by Hoechst staining, and two-step fusion of somatic cells from primary granulosa cell cultures with two cytoplasts. Blastocyst
Vajta, G. +4 more
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Cloning by function: expression cloning in mammalian cells
Trends in Pharmacological Sciences, 1994malian protein, the most important decision is the selection of a suitable strategy. Until recently, cloning re- quired prior purification of the pro- tein, and the corresponding cDNA was isolated by one of two approaches: (1) Micro-sequencing of all or parts of the purified protein was used to generate nucleic acid probes for ...
H, Simonsen, H F, Lodish
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1987
Publisher Summary This chapter describes techniques for obtaining monoclonal lines of antigen-specific T cells. Murine antigen-reactive T cell clones can be divided into three categories, based on their functional phenotype (cytolytic vs noncytolytic), and their ability to proliferate in response to antigen in the absence of an exogenous source of ...
A, Livingstone, C G, Fathman
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Publisher Summary This chapter describes techniques for obtaining monoclonal lines of antigen-specific T cells. Murine antigen-reactive T cell clones can be divided into three categories, based on their functional phenotype (cytolytic vs noncytolytic), and their ability to proliferate in response to antigen in the absence of an exogenous source of ...
A, Livingstone, C G, Fathman
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Embryonic and somatic cell cloning
Reproduction, Fertility and Development, 1999Revolutionary opportunities in biology, medicine and agriculture arise from the observation that offspring are obtained after nuclear transfer if somatic donor cells are induced to become quiescent. Exploitation of many of these opportunities will depend upon optimizing procedures for nuclear transfer.
I, Wilmut, L, Young, K H, Campbell
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Single cell sorting and cloning
Journal of Immunological Methods, 2000Cell sorters now allow the selection of cells and other bodies according to a range of quite diverse criteria. The additional refinement that allows the sorting of individual cells based on these criteria has seen application in many fields of research.
F L, Battye, A, Light, D M, Tarlinton
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Blood, 2006
AbstractThe detection of a monoclonal immunoglobulin in serum or urine usually raises concerns about the size of the underlying B-cell-derived clone and possible systemic effects caused by its expansion. However, a small clone can synthesize a very toxic protein, producing devastating systemic damage and protean clinical presentations.
MERLINI, GIAMPAOLO, STONE MJ
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AbstractThe detection of a monoclonal immunoglobulin in serum or urine usually raises concerns about the size of the underlying B-cell-derived clone and possible systemic effects caused by its expansion. However, a small clone can synthesize a very toxic protein, producing devastating systemic damage and protean clinical presentations.
MERLINI, GIAMPAOLO, STONE MJ
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Modular cloning in plant cells
Trends in Plant Science, 2005New plant genes are being discovered at a rapid pace. Yet, in most cases, their precise function remains elusive. The recent advent of recombinational cloning techniques has significantly improved our ability to investigate gene functions systematically.
Karimi, Mansour +2 more
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1998
The word “clone” is used to describe a cell population that has derived from a single cell. Such single cells are termed “clonogenic” and only a small proportion of normal cells and probably a higher proportion of tumor cells have the proliferative capabilities required to give rise to clones.
Shirley McBride +2 more
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The word “clone” is used to describe a cell population that has derived from a single cell. Such single cells are termed “clonogenic” and only a small proportion of normal cells and probably a higher proportion of tumor cells have the proliferative capabilities required to give rise to clones.
Shirley McBride +2 more
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1984
T cell clones are useful models for studying lymphocyte function both at the level of the individual cell and in interacting systems. Murine cytolytic and non- cytolyic T cell clones have been obtained with relative ease, and the particular procedure used to derive and maintain T cell clones may influence profoundly the characteristics of the resulting
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T cell clones are useful models for studying lymphocyte function both at the level of the individual cell and in interacting systems. Murine cytolytic and non- cytolyic T cell clones have been obtained with relative ease, and the particular procedure used to derive and maintain T cell clones may influence profoundly the characteristics of the resulting
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Cloning of fresh lymphoma cells
Medical and Pediatric Oncology, 1981AbstractWe attempted to induce in vivo clonal growth of neoplastic lymphoid cells from fresh specimens of involved tissue from 22 patients with non‐Hodgkin lymphoma (NHL). Conditioned media derived from two human B‐lymphocyte tissue culture lines were tested for their ability to promote colony growth.
M, Nishikori +3 more
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