Results 1 to 10 of about 86,371 (298)

An organism arises from every nucleus.

Folia Histochemica et Cytobiologica, 2009
The fact that, cloning using somatic cell nuclear transfer (SCNT) method has been performed, opened new horizons for cloning, and changed the way of our understanding and approach to cell and nucleus.
Nurullah Keklikoglu
doaj   +4 more sources

MOLECULAR CLONING OF OVINE cDNA LEPTIN GENE

Scientific Papers Animal Science and Biotechnologies, 2023
An efficient bacterial transformation system suitable for cloning the coding sequence of the ovine leptin gene in E. coli DH5α host cells using the pGEMT easy vector it is described in this paper.
CLAUDIA TEREZIA SOCOL, A. VLAIC, VIORICA COŞIER   +2 more
doaj   +1 more source

Apoptosis Gene Hunting Using Retroviral Expression Cloning: Identification of Vacuolar ATPase Subunit E [PDF]

The Scientific World Journal, 2003
Over the past 10-15 years there has been an explosion of interest in apoptosis. The delayed realisation that cell death is an essential part of life for any multicellular organism has meant that, despite the recent and rapid developments of the last ...
Claire L. Anderson, Gwyn T. Williams
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Expression capable library for studies of Neisseria gonorrhoeae, version 1.0 [PDF]

BMC Microbiology, 2005
Background The sexually transmitted disease, gonorrhea, is a serious health problem in developed as well as in developing countries, for which treatment continues to be a challenge.
Wachocki Susi, Prichard Kimberly, Clary Susan, Weyand Nathan J, Howie Heather, Jens Jason, Keller Thomas J, Langford Chris, Potter Laura, Friedrich Alexandra, Mason Roxie M, Du Ying, Altherr Michael R, Brettin Thomas, Sodergren Erica, Dillard Joseph P, Weinstock George, So Magdalene, Arvidson Cindy   +18 more
doaj   +2 more sources

A programmable pAgo nuclease with high activity and specificity for efficient DNA and RNA manipulation [PDF]

Communications Biology
Prokaryotic Argonaute proteins (pAgos) are programmable nucleases with potential for the development of novel nucleic acid manipulation tools. Previous studies have shown that several pAgos exhibit strong preferences for either DNA or RNA targets, and ...
Jiao Hu, Yangyang Hu, Yang Wang, Hengrong Jiang, Meng Chen, Jingjing Zhang, Fajun Lai, Xiu Li, Yibin Cheng, Tao Liu, Guoli Yang, Fei Wang, Wenqiang Li, Lixin Ma   +13 more
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Whole Organism Cloning [PDF]

, 2020

core   +2 more sources

Whole Organism Cloning

, 2001
J.B. Gurdon
core   +3 more sources

An Optimized Transformation Protocol for Escherichia coli BW3KD with Supreme DNA Assembly Efficiency

Microbiology Spectrum, 2022
DNA cloning requires two steps: the assembly of recombinant DNA molecules and the transformation of the product into a host organism for replication. High efficiencies in both processes can increase the success rate. Recently, we developed an Escherichia
Yuqing Yang, Menghui Liu, Tianqi Wang, Qian Wang, Huaiwei Liu, Luying Xun, Yongzhen Xia   +6 more
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Simple Growth Complementation Assay in Yeast

Bio-Protocol, 2023
The study of genes and their products is an essential prerequisite for fundamental research. Characterization can be achieved by analyzing mutants or overexpression lines or by studying the localization and substrate specificities of the resulting ...
Robert Fuhrmeister, Jana Streubel
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Alternative PCR-Based Approaches for Generation of Komagataella phaffii Strains

Microorganisms, 2023
Komagataella phaffii (Pichia pastoris) is a widely known microbial host for recombinant protein production and an emerging model organism in fundamental research. The development of new materials and techniques on this yeast improves heterologous protein
Anastasiya Makeeva, Dmitry Muzaev, Maria Shubert, Tatiana Ianshina, Anton Sidorin, Elena Sambuk, Andrey Rumyantsev, Marina Padkina   +7 more
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