Biotechnological bases of the development of cloned pig embryos
The term ‘clone’ in animal biotechnology refers to an organism derived from non-sexual reproduction, which is both a direct offspring and a genetic copy of the parent organism.
A. V. Lopukhov +2 more
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MiR-433 and miR-127 arise from independent overlapping primary transcripts encoded by the miR-433-127 locus. [PDF]
MicroRNAs play significant roles in development, metabolism and carcinogenesis, however, limited information is available about their primary transcripts and the transcriptional regulation of the microRNA genes.
Guisheng Song, Li Wang
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tRNA genes in mycobacteria: organization and molecular cloning
DNAs from nine mycobacteria cleaved with restriction endonucleases were hybridized with cDNA probes synthesized to tRNAs from Mycobacterium tuberculosis and Mycobacterium smegmatis. The tRNA genes are conserved, but their gross genomic organization has diverged in six of the nine species examined. Organisms of the M.
S, Bhargava, A K, Tyagi, J S, Tyagi
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Transcriptional organization of a cloned chemotaxis locus of Bacillus subtilis [PDF]
A cloned chemotaxis operon has been characterized. Thirteen representative che mutations from different complementation groups were localized on the physical map by recombination experiments. The use of integration plasmids established that at least 10 of these complementation groups within this locus are cotranscribed.
A R, Zuberi +3 more
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Purification and Cloning of Lipase from Micro-Organism [PDF]
Lipases are a class of serine hydrolase, which belongs to the α/β hydrolases super family. Triacylglycerol acyl esters hydrolase are carboxyl esterase that catalyses both hydrolysis and synthesis of ester formed from glycerol. Lipase enzyme that catalyzes the hydrolysis of stored triacylglycerol releasing di and monoglycerol.
Heba Elgendy, Hanan Hassan, Atef Mohamed
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Molecular Studies on Preproinsulin Gene
Insulin plays an important role in maintaining the blood glucose level of the body. The β-cells of pancreas produce insulin in the form of precursor that is preproinsulin.
Sabir Sarah, Younas Hooria
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Cloning, organization, and expression of the bioluminescence genes of Xenorhabdus luminescens [PDF]
The lux genes of Xenorhabdus luminescens, a symbiont of the nematode Heterorhabditis bacteriophora, were cloned and expressed in Escherichia coli. The expression of these genes in E. coli was qualitatively similar to their expression in X. luminescens. The organization of the genes is similar to that found in the marine luminous bacteria. Hybridization
S, Frackman, M, Anhalt, K H, Nealson
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Estimating genetic variability in non-model taxa: a general procedure for discriminating sequence errors from actual variation. [PDF]
Genetic variation is the driving force of evolution and as such is of central interest for biologists. However, inadequate discrimination of errors from true genetic variation could lead to incorrect estimates of gene copy number, population genetic ...
Karen Dawson +2 more
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Microarray Assistant Clone Organizer and Array Simulator [PDF]
Microarrays are extensively used in molecular biology experiments. While several vendors offer microarrays on a variety of platforms, many researchers prefer to use custom microarrays with a selected list of clones for their experiments. Many research centers have established core facilities for the production of custom microarrays.
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Physiology, structure, and regulation of the cloned organic anion transporters [PDF]
1. The transport of negatively charged drugs, xenobiotics, and metabolites by epithelial tissues, particularly the kidney, plays critical roles in controlling their distribution, concentration, and retention in the body. Thus, organic anion transporters (OATs) impact both their therapeutic efficacy and potential toxicity. 2.
C, Srimaroeng +2 more
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