Molecular Studies on Preproinsulin Gene
Insulin plays an important role in maintaining the blood glucose level of the body. The β-cells of pancreas produce insulin in the form of precursor that is preproinsulin.
Sabir Sarah, Younas Hooria
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Cloning, organization, and expression of the bioluminescence genes of Xenorhabdus luminescens [PDF]
The lux genes of Xenorhabdus luminescens, a symbiont of the nematode Heterorhabditis bacteriophora, were cloned and expressed in Escherichia coli. The expression of these genes in E. coli was qualitatively similar to their expression in X. luminescens. The organization of the genes is similar to that found in the marine luminous bacteria. Hybridization
S, Frackman, M, Anhalt, K H, Nealson
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Estimating genetic variability in non-model taxa: a general procedure for discriminating sequence errors from actual variation. [PDF]
Genetic variation is the driving force of evolution and as such is of central interest for biologists. However, inadequate discrimination of errors from true genetic variation could lead to incorrect estimates of gene copy number, population genetic ...
Karen Dawson +2 more
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tRNA genes in mycobacteria: organization and molecular cloning
DNAs from nine mycobacteria cleaved with restriction endonucleases were hybridized with cDNA probes synthesized to tRNAs from Mycobacterium tuberculosis and Mycobacterium smegmatis. The tRNA genes are conserved, but their gross genomic organization has diverged in six of the nine species examined. Organisms of the M.
S, Bhargava, A K, Tyagi, J S, Tyagi
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Physiology, structure, and regulation of the cloned organic anion transporters [PDF]
1. The transport of negatively charged drugs, xenobiotics, and metabolites by epithelial tissues, particularly the kidney, plays critical roles in controlling their distribution, concentration, and retention in the body. Thus, organic anion transporters (OATs) impact both their therapeutic efficacy and potential toxicity. 2.
C, Srimaroeng +2 more
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Shotgun Phage Display - Selection for Bacterial Receptins or other Exported Proteins
Shotgun phage display cloning involves construction of libraries from randomly fragmented bacterial chromosomal DNA, cloned genes, or eukaryotic cDNAs, into a phagemid vector.
Jacobsson Karin +3 more
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Microarray Assistant Clone Organizer and Array Simulator [PDF]
Microarrays are extensively used in molecular biology experiments. While several vendors offer microarrays on a variety of platforms, many researchers prefer to use custom microarrays with a selected list of clones for their experiments. Many research centers have established core facilities for the production of custom microarrays.
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Repetitive sequences and their organization on genomic clones of Zea mays [PDF]
Fourteen recombinant clones from Zea mays were studied with regard to their composition of unique and repetitive sequences. Southern hybridization experiments were used to classify restriction fragments of the clones into a unique, middle or highly repetitive class of reiteration frequency.
M, Gupta +3 more
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Transcriptional organization of a cloned chemotaxis locus of Bacillus subtilis [PDF]
A cloned chemotaxis operon has been characterized. Thirteen representative che mutations from different complementation groups were localized on the physical map by recombination experiments. The use of integration plasmids established that at least 10 of these complementation groups within this locus are cotranscribed.
A R, Zuberi +3 more
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Emerging technologies to create inducible and genetically defined porcine cancer models
There is an emerging need for new animal models that address unmet translational cancer research requirements. Transgenic porcine models provide an exceptional opportunity due to their genetic, anatomic and physiological similarities with humans.
Lawrence B Schook +6 more
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