MiR-433 and miR-127 arise from independent overlapping primary transcripts encoded by the miR-433-127 locus. [PDF]
PLoS ONE, 2008 MicroRNAs play significant roles in development, metabolism and carcinogenesis, however, limited information is available about their primary transcripts and the transcriptional regulation of the microRNA genes.
Guisheng Song, Li Wang
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Molecular Studies on Preproinsulin Gene
MATEC Web of Conferences, 2016 Insulin plays an important role in maintaining the blood glucose level of the body. The β-cells of pancreas produce insulin in the form of precursor that is preproinsulin.
Sabir Sarah, Younas Hooria
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tRNA genes in mycobacteria: organization and molecular cloning
Journal of Bacteriology, 1990 DNAs from nine mycobacteria cleaved with restriction endonucleases were hybridized with cDNA probes synthesized to tRNAs from Mycobacterium tuberculosis and Mycobacterium smegmatis. The tRNA genes are conserved, but their gross genomic organization has diverged in six of the nine species examined. Organisms of the M.
S, Bhargava, A K, Tyagi, J S, Tyagi
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High-Throughput Cloning of Campylobacter jejuni ORFs by in Vivo Recombination in Escherichia coli [PDF]
, 2016 A rate-limiting and costly step in many proteomics analyses is the cloning of all of the ORFs for an organism into technique-specific vectors. Here, we describe the generation of a Campylobacter jejuni expression clone set using a high-throughput cloning
Russell L. Finley (2715226) +5 more
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Estimating genetic variability in non-model taxa: a general procedure for discriminating sequence errors from actual variation. [PDF]
PLoS ONE, 2010 Genetic variation is the driving force of evolution and as such is of central interest for biologists. However, inadequate discrimination of errors from true genetic variation could lead to incorrect estimates of gene copy number, population genetic ...
Karen Dawson +2 more
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CReasPy-Cloning: A Method for Simultaneous Cloning and Engineering of Megabase-Sized Genomes in Yeast Using the CRISPR-Cas9 System [PDF]
, 2019 Over the past decade, a new strategy was developed to bypass the difficulties to genetically engineer some microbial species by transferring (or “cloning”) their genome into another organism that is amenable to efficient genetic modifications and ...
Vincent Talenton (7579148) +8 more
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Shotgun Phage Display - Selection for Bacterial Receptins or other Exported Proteins
Biological Procedures Online, 2003 Shotgun phage display cloning involves construction of libraries from randomly fragmented bacterial chromosomal DNA, cloned genes, or eukaryotic cDNAs, into a phagemid vector.
Jacobsson Karin +3 more
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Repetitive sequences and their organization on genomic clones of Zea mays [PDF]
The EMBO Journal, 1984 Fourteen recombinant clones from Zea mays were studied with regard to their composition of unique and repetitive sequences. Southern hybridization experiments were used to classify restriction fragments of the clones into a unique, middle or highly repetitive class of reiteration frequency.
M, Gupta +3 more
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Transcriptional organization of a cloned chemotaxis locus of Bacillus subtilis [PDF]
Journal of Bacteriology, 1990 A cloned chemotaxis operon has been characterized. Thirteen representative che mutations from different complementation groups were localized on the physical map by recombination experiments. The use of integration plasmids established that at least 10 of these complementation groups within this locus are cotranscribed.
A R, Zuberi +3 more
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High-throughput cloning and expression in recalcitrant bacteria [PDF]
, 2007 We developed a generic method for high-throughput cloning in bacteria that are less amenable to conventional DNA manipulations. The method involves ligation-independent cloning in an intermediary Escherichia coli vector, which is rapidly converted via ...
Geertsma, Eric R. +3 more
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