Results 11 to 20 of about 86,543 (295)
MOLECULAR CLONING OF OVINE cDNA LEPTIN GENE [PDF]
Scientific Papers Animal Science and Biotechnologies, 2023 An efficient bacterial transformation system suitable for cloning the coding sequence of the ovine leptin gene in E. coli DH5α host cells using the pGEMT easy vector it is described in this paper.
CLAUDIA TEREZIA SOCOL +2 more
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Apoptosis Gene Hunting Using Retroviral Expression Cloning: Identification of Vacuolar ATPase Subunit E [PDF]
The Scientific World Journal, 2003 Over the past 10-15 years there has been an explosion of interest in apoptosis. The delayed realisation that cell death is an essential part of life for any multicellular organism has meant that, despite the recent and rapid developments of the last ...
Claire L. Anderson, Gwyn T. Williams
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Expression capable library for studies of
BMC Microbiology, 2005 Background The sexually transmitted disease, gonorrhea, is a serious health problem in developed as well as in developing countries, for which treatment continues to be a challenge.
Wachocki Susi +18 more
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Generating a custom TA-cloning expression plasmid for Lactococcus lactis
BioTechniques, 2012 The growing popularity of the lactic acid bacterium Lactococcus lactis has increased demand for novel high-throughput cloning methods. Here we describe a general TA-cloning methodology and demonstrate its feasibility using the plasmid pNZ8148.
Aleš Berlec, Borut Štrukelj
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Means and methods for cloning nucleic acid sequences [PDF]
, 2008 The invention provides means and methods for efficiently cloning nucleic acid sequences of interest in micro-organisms that are less amenable to conventional nucleic acid manipulations, as compared to, for instance, E.coli.
Geertsma, Eric Robin, Poolman, Berend
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Module-based construction of plasmids for chromosomal integration of the fission yeast Schizosaccharomyces pombe [PDF]
Open Biology, 2015 Integration of an external gene into a fission yeast chromosome is useful to investigate the effect of the gene product. An easy way to knock-in a gene construct is use of an integration plasmid, which can be targeted and inserted to a chromosome through
Yasutaka Kakui +7 more
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Heterologous expression of Cysteine Protease 8 from Trichomonas foetus in Pichia pastoris [PDF]
Journal of Veterinary and Animal Sciences, 2023 Bovine trichomonosis is one of the most neglected venereal diseases of cattle. Trichomonas foetus, the causative organism was known over decades and is responsible for severe reproductive failure.
Karli Geethanjali +2 more
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A Simplified Method for CRISPR-Cas9 Engineering of Bacillus subtilis
Microbiology Spectrum, 2021 The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system from Streptococcus pyogenes has been widely deployed as a tool for bacterial strain construction.
Ankita J. Sachla +2 more
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Biotechnological bases of the development of cloned pig embryos
Вавиловский журнал генетики и селекции, 2019 The term ‘clone’ in animal biotechnology refers to an organism derived from non-sexual reproduction, which is both a direct offspring and a genetic copy of the parent organism.
A. V. Lopukhov +2 more
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