Results 11 to 20 of about 86,371 (298)
Implication of therapeutic cloning for organ transplantation [PDF]
European Journal of Cardio-Thoracic Surgery, 2004 Replacement of damaged myocardium with electrically functional, contracting syncytium with a balanced blood supply remains a key goal for the treatment of hearts damaged by coronary heart disease or other disorders. Stem cell therapy offers a potential solution.
J HESCHELER +9 more
openaire +3 more sources
Applied Sciences, 2023 Swine is a common model organism for biomedical research. Epigenetic reprogramming in somatic cell nuclear transfer (SCNT) embryos does not fully recapitulate the natural DNA demethylation events at fertilisation.
Maciej Grzybek +7 more
doaj +1 more source
Cloning, Genomic Organization, and Characterization of a Human Cholinephosphotransferase [PDF]
Journal of Biological Chemistry, 2000 A cholinephosphotransferase activity catalyzes the final step in the de novo synthesis of phosphatidylcholine via the transfer of a phosphocholine moiety from CDP choline to diacylglycerol. Ethanolaminephosphotransferase activity catalyzes a similar reaction substituting CDP ethanolamine as the phosphobase donor.
A L, Henneberry +2 more
openaire +2 more sources
Primary fibroblast cell cycle synchronization and effects on handmade cloned (HMC) bovine embryos
Ciência Animal Brasileira, 2018 Spatial and temporal synchrony and compatibility between the receptor oocyte and the donor cell nucleus are necessary for the process of embryo cloning to allow nuclear reprogramming and early embryonic development. The objective of the present study was
Natalia Andrea Gómez +2 more
doaj +1 more source
Blasticidin S Deaminase: A New Efficient Selectable Marker for Chlamydomonas reinhardtii
Frontiers in Plant Science, 2020 Chlamydomonas reinhardtii is a model unicellular organism for basic or biotechnological research, such as the production of high-value molecules or biofuels thanks to its photosynthetic ability.
Félix de Carpentier +7 more
doaj +1 more source
Generating a custom TA-cloning expression plasmid for Lactococcus lactis
BioTechniques, 2012 The growing popularity of the lactic acid bacterium Lactococcus lactis has increased demand for novel high-throughput cloning methods. Here we describe a general TA-cloning methodology and demonstrate its feasibility using the plasmid pNZ8148.
Aleš Berlec, Borut Štrukelj
doaj +1 more source
Means and methods for cloning nucleic acid sequences [PDF]
, 2008 The invention provides means and methods for efficiently cloning nucleic acid sequences of interest in micro-organisms that are less amenable to conventional nucleic acid manipulations, as compared to, for instance, E.coli.
Geertsma, Eric Robin, Poolman, Berend
core +2 more sources
Heterologous expression of Cysteine Protease 8 from Trichomonas foetus in Pichia pastoris [PDF]
Journal of Veterinary and Animal Sciences, 2023 Bovine trichomonosis is one of the most neglected venereal diseases of cattle. Trichomonas foetus, the causative organism was known over decades and is responsible for severe reproductive failure.
Karli Geethanjali +2 more
doaj +1 more source
Module-based construction of plasmids for chromosomal integration of the fission yeast Schizosaccharomyces pombe [PDF]
Open Biology, 2015 Integration of an external gene into a fission yeast chromosome is useful to investigate the effect of the gene product. An easy way to knock-in a gene construct is use of an integration plasmid, which can be targeted and inserted to a chromosome through
Yasutaka Kakui +7 more
doaj +1 more source
A Simplified Method for CRISPR-Cas9 Engineering of Bacillus subtilis
Microbiology Spectrum, 2021 The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system from Streptococcus pyogenes has been widely deployed as a tool for bacterial strain construction.
Ankita J. Sachla +2 more
doaj +1 more source