Results 11 to 20 of about 437,074 (308)

Towards an understanding of British public attitudes concerning human cloning. [PDF]

open access: yes, 2007
The ability of scientists to apply cloning technology to humans has provoked public discussion and media coverage. The present paper reports on a series of studies examining public attitudes to human cloning in the UK, bringing together a range of ...
Coyle, Adrian   +13 more
core   +5 more sources

Module-based construction of plasmids for chromosomal integration of the fission yeast Schizosaccharomyces pombe [PDF]

open access: yesOpen Biology, 2015
Integration of an external gene into a fission yeast chromosome is useful to investigate the effect of the gene product. An easy way to knock-in a gene construct is use of an integration plasmid, which can be targeted and inserted to a chromosome through
Yasutaka Kakui   +7 more
doaj   +1 more source

Heterologous expression of Cysteine Protease 8 from Trichomonas foetus in Pichia pastoris [PDF]

open access: yesJournal of Veterinary and Animal Sciences, 2023
Bovine trichomonosis is one of the most neglected venereal diseases of cattle. Trichomonas foetus, the causative organism was known over decades and is responsible for severe reproductive failure.
Karli Geethanjali   +2 more
doaj   +1 more source

A Simplified Method for CRISPR-Cas9 Engineering of Bacillus subtilis

open access: yesMicrobiology Spectrum, 2021
The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system from Streptococcus pyogenes has been widely deployed as a tool for bacterial strain construction.
Ankita J. Sachla   +2 more
doaj   +1 more source

MOLECULAR CLONING OF OVINE cDNA LEPTIN GENE

open access: yesScientific Papers Animal Science and Biotechnologies, 2023
An efficient bacterial transformation system suitable for cloning the coding sequence of the ovine leptin gene in E. coli DH5α host cells using the pGEMT easy vector it is described in this paper.
CLAUDIA TEREZIA SOCOL   +2 more
doaj  

Biotechnological bases of the development of cloned pig embryos

open access: yesВавиловский журнал генетики и селекции, 2019
The term ‘clone’ in animal biotechnology refers to an organism derived from non-sexual reproduction, which is both a direct offspring and a genetic copy of the parent organism.
A. V. Lopukhov   +2 more
doaj   +1 more source

MiR-433 and miR-127 arise from independent overlapping primary transcripts encoded by the miR-433-127 locus. [PDF]

open access: yesPLoS ONE, 2008
MicroRNAs play significant roles in development, metabolism and carcinogenesis, however, limited information is available about their primary transcripts and the transcriptional regulation of the microRNA genes.
Guisheng Song, Li Wang
doaj   +1 more source

Purification and Cloning of Lipase from Micro-Organism [PDF]

open access: yesJournal of Current Veterinary Research, 2020
Lipases are a class of serine hydrolase, which belongs to the α/β hydrolases super family. Triacylglycerol acyl esters hydrolase are carboxyl esterase that catalyses both hydrolysis and synthesis of ester formed from glycerol. Lipase enzyme that catalyzes the hydrolysis of stored triacylglycerol releasing di and monoglycerol.
Heba Elgendy, Hanan Hassan, Atef Mohamed
openaire   +1 more source

CLONING

open access: yes, 2006
Foreword -- Introduction -- Ch. 1. Should reproductive cloning be pursued? -- 1. Reproductive cloning is ethical / Gregory E. Pence -- 2. Reproductive cloning is unethical / Peter J. Cataldo -- 3.
Gerdes, Louise, ed.
core   +3 more sources

Apoptosis Gene Hunting Using Retroviral Expression Cloning: Identification of Vacuolar ATPase Subunit E

open access: yesThe Scientific World Journal, 2003
Over the past 10-15 years there has been an explosion of interest in apoptosis. The delayed realisation that cell death is an essential part of life for any multicellular organism has meant that, despite the recent and rapid developments of the last ...
Claire L. Anderson, Gwyn T. Williams
doaj   +1 more source

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