Results 31 to 40 of about 86,371 (298)
Shotgun Phage Display - Selection for Bacterial Receptins or other Exported Proteins
Biological Procedures Online, 2003 Shotgun phage display cloning involves construction of libraries from randomly fragmented bacterial chromosomal DNA, cloned genes, or eukaryotic cDNAs, into a phagemid vector.
Jacobsson Karin +3 more
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High-throughput cloning and expression in recalcitrant bacteria [PDF]
, 2007 We developed a generic method for high-throughput cloning in bacteria that are less amenable to conventional DNA manipulations. The method involves ligation-independent cloning in an intermediary Escherichia coli vector, which is rapidly converted via ...
Geertsma, Eric R. +3 more
core +1 more source
67 ORGAN WEIGHT VARIATION IN CLONED PIGS [PDF]
Reproduction, Fertility and Development, 2004 Previously, we compared adult clones and naturally bred control females using a series of physiological and genetic parameters and found considerable variation in certain traits in clones. In order to more fully understand this clonal variation we examined the organ weight differences among a group of clones from a single litter.
J.A. Piedrahita +2 more
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Emerging technologies to create inducible and genetically defined porcine cancer models
Frontiers in Genetics, 2016 There is an emerging need for new animal models that address unmet translational cancer research requirements. Transgenic porcine models provide an exceptional opportunity due to their genetic, anatomic and physiological similarities with humans.
Lawrence B Schook +6 more
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Biotechnology for Biofuels and Bioproducts, 2022 Background The robust model cyanobacterium Synechocystis PCC 6803 is increasingly explored for its potential to use solar energy, water and atmospheric CO2 for the carbon-neutral production of terpenes, the high-value chemicals that can be used for the ...
Victoire Blanc-Garin +6 more
doaj +1 more source
Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system
FEBS Letters, EarlyView.Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley +1 more source
Embryos, Clones, and Stem Cells: A Scientific Primer
The Scientific World Journal, 2004 This article is intended to give the nonspecialist an insight into the nuances of “clones”, cloning, and stem cells. It distinguishes embryonic and adult stem cells, their normal function in the organism, their origin, and how they are recovered to ...
Kenyon S. Tweedell
doaj +1 more source
Molecular Plant-Microbe Interactions, 2002 Conditions have been developed for genetic transformation and insertional mutagenesis in Leifsonia xyli subsp. xyli (Lxx), the causal organism of ratoon stunting disease (RSD), one of the most damaging and intractable diseases of sugarcane ...
Stevens M. Brumbley +3 more
doaj +1 more source
FEBS Letters, EarlyView.This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley +1 more source
Molecular cloning of integrated simian sarcoma virus: genome organization of infectious DNA clones. [PDF]
Proceedings of the National Academy of Sciences, 1981 The integrated form of simian sarcoma virus (SSV) was molecularly cloned in the Charon 16A strain of bacteriophage lambda. In transfection analysis, the recombinant viral DNAs demonstrated the ability to transform cells in tissue culture at high efficiency.
K C, Robbins, S G, Devare, S A, Aaronson
openaire +2 more sources