Results 51 to 60 of about 86,371 (298)
FEBS Open Bio, EarlyView.CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
High-Throughput Cloning of Campylobacter jejuni ORFs by in Vivo Recombination in Escherichia coli
, 2016 A rate-limiting and costly step in many proteomics analyses is the cloning of all of the ORFs for an organism into technique-specific vectors. Here, we describe the generation of a Campylobacter jejuni expression clone set using a high-throughput cloning
Russell L. Finley (2715226) +5 more
core +3 more sources
A uniform cloning platform for mycobacterial genetics and protein production [PDF]
, 2018 Molecular research on mycobacteria relies on a multitude of tools for the genetic manipulation of these clinically important bacteria. However, a uniform set of vectors allowing for standardized cloning procedures is not available.
Sille Remm +17 more
core +1 more source
Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains
FEBS Open Bio, EarlyView.The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth +6 more
wiley +1 more source
, 2019 Over the past decade, a new strategy was developed to bypass the difficulties to genetically engineer some microbial species by transferring (or “cloning”) their genome into another organism that is amenable to efficient genetic modifications and ...
Vincent Talenton (7579148) +8 more
core +2 more sources
Humanidades Médicas, 2003 La biotecnología, aunque tan antigua como la invención del vino y la fermentación de la cerveza por el hombre, tiene en los años 60 del siglo XX, un importante despegue con las técnicas de biología molecular y clonaje de genes foráneos en organismos ...
Ayní Rodríguez Pargas +3 more
doaj
Mos1 Element-Mediated CRISPR Integration of Transgenes in Caenorhabditis elegans
G3: Genes, Genomes, Genetics, 2019 The introduction of exogenous genes in single-copy at precise genomic locations is a powerful tool that has been widely used in the model organism Caenorhabditis elegans.
Nicholas S. Philip +4 more
doaj +1 more source
Development of human monoclonal antibodies against TARM1 by yeast display
FEBS Open Bio, EarlyView.Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe +5 more
wiley +1 more source
Microbiology SpectrumImprovements in super-resolution and single-molecule techniques, along with the development of new fluorescent proteins and labeling methods, have allowed super-resolution imaging of bacterial cells.
Ipek Altinoglu, Rut Carballido-Lopez
doaj +1 more source
Carbonic anhydrase 5 regulates acid-base homeostasis in zebrafish.
PLoS ONE, 2012 The regulation of the acid-base balance in cells is essential for proper cellular homeostasis. Disturbed acid-base balance directly affects cellular physiology, which often results in various pathological conditions. In every living organism, the protein
Ruben Postel, Arnoud Sonnenberg
doaj +1 more source