Results 61 to 70 of about 86,371 (298)

Triple-barrel Organization of ENaC, a Cloned Epithelial Na+ Channel [PDF]

Journal of Biological Chemistry, 1996
A cloned rat epithelial Na+ channel (rENaC) was studied in planar lipid bilayers. Two forms of the channel were examined: channels produced by the alpha subunit alone and those formed by alpha, beta, and gamma subunits. The protein was derived from two sources: either from in vitro translation reaction followed by Sephadex column purification or from ...
I I, Ismailov, M S, Awayda, B K, Berdiev, J K, Bubien, J E, Lucas, C M, Fuller, D J, Benos   +6 more
openaire   +2 more sources

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

The crystal structure of the Borrelia burgdorferi nicotinamidase BBE22 resolves a long‐standing annotation error

FEBS Open Bio, EarlyView.
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley   +1 more source

Mapping the Trypanosoma cruzi Genome: Analyses of Representative Cosmid Libraries

BioTechniques, 1996
In order to generate contiguous cosmid coverage of the genome of the protozoan parasite Trypanosoma cruzi for large-scale sequence analysis, a cosmid library of 36864 individual, primary clones was generated.
J. Hanke, D.O. Sánchez, J. Henriksson, L. Åslund, U. Pettersson, A.C.C. Frasch, J.D. Hoheisel   +6 more
doaj   +1 more source

Identification, cloning and expressions of proteases from a cold adapted organism Aliivibrio salmonicida [PDF]

, 2009
The work presented in this thesis provides an overview of molecular and structural biology projects and their related problems. The problems arises during these projects are common for most of the target proteins, with varying intensity of severity.
Ather, Arjumand
core  

A 17 Year Old With Developmental Delay Presenting With Increasing Confusion and Imbalance

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Methylmalonic acidemia is an autosomal recessive genetic disorder primarily caused by defects in methylmalonyl‐CoA mutase and cobalamin (vitamin B12) metabolism. These defects disrupt the tricarboxylic acid cycle and oxidative phosphorylation, leading to the abnormal accumulation of metabolic products such as methylmalonic acid, propionic acid,
Wei Zhao, Yingli Zhang, Hongliang Zheng
wiley   +1 more source

Aptamer‐Based Delivery Systems for VEGF and NGF Modulation in Ocular Therapies

Advanced Healthcare Materials, EarlyView.
Aptamer‐based delivery systems targeting NGF and VEGF hold significant potential for precise and sustained treatment of complex diseases affecting both the anterior and posterior segments of the eye. This review critically summarizes current strategies for NGF and VEGF delivery as well as VEGF sequestration, providing a clinical perspective on how ...
Nadine Best, Gizem Karabiyik, Gerd Geerling, Sarah Barbara Zwingelberg, Gregor Lang, Martin Humenik   +5 more
wiley   +1 more source

Editorial - Human cloning: the ethical challenge

, 2002
In November of 2001 Advanced Cell Technology, a company based in Massachusetts, USA, announced the first known attempt to clone a human organism. For many people following recent developments in biotechnology this did not come as a total surprise.
Alfonso Gómez-Lobo
core   +2 more sources

Seabream ghrelin: cDNA cloning, genomic organization and promoter studies [PDF]

Journal of Endocrinology, 2006
Recent studies have indicated that ghrelin stimulates growth hormone release from the pituitary via the growth hormone secretagogue receptor (GHSR). We have previously isolated two GHSR subtypes from the pituitary of the black seabream Acanthopagrus schlegeli.
Yeung, Chung M., Cheng, C. H K, Woo, N. Y S, Chan, Chi B.   +3 more
openaire   +4 more sources

One‐Step Microfluidic Manufactured Fucose‐Decorated Sweetosomes Choose the Time and the Road for Their Intracellular Journey to Cancer Treatment

Advanced Healthcare Materials, EarlyView.
Sweetosomes, a new class of fucose‐decorated liposomes, are developed via a one‐step microfluidic process without surface chemistry. This study elucidates their main caveolae‐mediated entry and distinct endosomal trafficking. These nanostructures demonstrate superior endosomal escape, organelle acidity modulation, and prolonged plasma persistence ...
Mattia Tiboni, Mariele Montanari, Shiva Khorshid, Michele Verboni, Andrea Duranti, Simone Lucarini, Daniele Lopez, Annalisa Aluigi, Gianluca Morganti, Michele Menotta, Giovanna Panza, Daniel J. Klionsky, Barbara Canonico, Luca Casettari   +13 more
wiley   +1 more source