Results 61 to 70 of about 184,561 (308)
Humanidades Médicas, 2003 La biotecnología, aunque tan antigua como la invención del vino y la fermentación de la cerveza por el hombre, tiene en los años 60 del siglo XX, un importante despegue con las técnicas de biología molecular y clonaje de genes foráneos en organismos ...
Ayní Rodríguez Pargas +3 more
doaj
Mos1 Element-Mediated CRISPR Integration of Transgenes in Caenorhabditis elegans
G3: Genes, Genomes, Genetics, 2019 The introduction of exogenous genes in single-copy at precise genomic locations is a powerful tool that has been widely used in the model organism Caenorhabditis elegans.
Nicholas S. Philip +4 more
doaj +1 more source
Triple-barrel Organization of ENaC, a Cloned Epithelial Na+ Channel [PDF]
Journal of Biological Chemistry, 1996 A cloned rat epithelial Na+ channel (rENaC) was studied in planar lipid bilayers. Two forms of the channel were examined: channels produced by the alpha subunit alone and those formed by alpha, beta, and gamma subunits. The protein was derived from two sources: either from in vitro translation reaction followed by Sephadex column purification or from ...
I I, Ismailov +6 more
openaire +2 more sources
FEBS Open Bio, EarlyView.dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon +6 more
wiley +1 more source
Microbiology SpectrumImprovements in super-resolution and single-molecule techniques, along with the development of new fluorescent proteins and labeling methods, have allowed super-resolution imaging of bacterial cells.
Ipek Altinoglu, Rut Carballido-Lopez
doaj +1 more source
Carbonic anhydrase 5 regulates acid-base homeostasis in zebrafish.
PLoS ONE, 2012 The regulation of the acid-base balance in cells is essential for proper cellular homeostasis. Disturbed acid-base balance directly affects cellular physiology, which often results in various pathological conditions. In every living organism, the protein
Ruben Postel, Arnoud Sonnenberg
doaj +1 more source
A novel platform for the production of nonhydroxylated gelatins based on the methylotrophic yeast Hansenula polymorpha [PDF]
, 2007 The use of yeast as a host for heterologous expression of proteins that are normally derived from animal tissue is a promising way to ensure defined products that are devoid of potential harmful animal side products.
Boer, Arjo L. de, +4 more
core +2 more sources
Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains
FEBS Open Bio, EarlyView.The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth +6 more
wiley +1 more source
Development of human monoclonal antibodies against TARM1 by yeast display
FEBS Open Bio, EarlyView.Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe +5 more
wiley +1 more source
Mapping the Trypanosoma cruzi Genome: Analyses of Representative Cosmid Libraries
BioTechniques, 1996 In order to generate contiguous cosmid coverage of the genome of the protozoan parasite Trypanosoma cruzi for large-scale sequence analysis, a cosmid library of 36864 individual, primary clones was generated.
J. Hanke +6 more
doaj +1 more source