Results 71 to 80 of about 86,543 (295)
Mapping the Trypanosoma cruzi Genome: Analyses of Representative Cosmid Libraries
BioTechniques, 1996 In order to generate contiguous cosmid coverage of the genome of the protozoan parasite Trypanosoma cruzi for large-scale sequence analysis, a cosmid library of 36864 individual, primary clones was generated.
J. Hanke +6 more
doaj +1 more source
A yeast model of 5‐oxoproline accumulation reveals a general toleration to 5‐oxoproline
FEBS Open Bio, EarlyView.Using a yeast model, we show that even high accumulation of 5‐oxoproline causes only mild cellular stress and does not trigger oxidative stress. Instead, cells adapt by activating efflux pumps and diverse protective pathways, suggesting that previously proposed harmful effects of 5‐oxoproline may arise from indirect metabolic imbalances rather than the
Pratiksha Dubey +4 more
wiley +1 more source
Identification, cloning and expressions of proteases from a cold adapted organism Aliivibrio salmonicida [PDF]
, 2009 The work presented in this thesis provides an overview of molecular and structural biology projects and their related problems. The problems arises during these projects are common for most of the target proteins, with varying intensity of severity.
Ather, Arjumand
core
FEBS Open Bio, EarlyView.The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley +1 more source
CRISPR Guide RNA Cloning for Mammalian Systems [PDF]
, 2020 The outlined protocol describes streamlined methods for the efficient and cost-effective generation of Cas9-associated guide RNAs. Two alternative strategies for guide RNA (gRNA) cloning are outlined based on the usage of the Type IIS restriction enzyme ...
Cher Yeo, Nan +7 more
core +1 more source
Effect of terahertz irradiation on DNA damage repair in living cells
FEBS Open Bio, EarlyView.We investigated the effect of terahertz (THz) wave irradiation on DNA double‐strand break (DSB) repair in living cells. We found that THz irradiation enhanced DSB repair at specific frequencies, whereas heat treatment inhibited it, indicating that this effect is nonthermal and frequency‐specific.
Yuya Ueno +3 more
wiley +1 more source
Gene Cloning Part 2: Making and Screening Gene Libraries [PDF]
, 2003 This lesson teaches how a specific gene can be identified from among the thousands of genes that can be cloned from an organism. Objectives for Gene Cloning Part 2 At the conclusion of this lesson you should be able to: Describe the library screening ...
Lee, Don, Hain, Patricia
core
Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level
Aging and Cancer, EarlyView.This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley +1 more source
CReasPy-Cloning: a method for simultaneous cloning and engineering of megabase-sized genomes in yeast using the CRISPR-Cas9 system. [PDF]
, 2018 Over the past decade, a new strategy was developed to bypass the difficulties to genetically engineer some microbial species by transferring (or "cloning") their genome into another organism that is amenable to efficient genetic modifications ...
Ruiz, Estelle +9 more
core +1 more source
Biochemistry of microbial itaconic acid production
Frontiers in Microbiology, 2013 Itaconic acid is an unsatured dicarbonic acid which has a high potential as a biochemical building block, because it can be used as a monomer for the production of a plethora of products including resins, plastics, paints and synthetic fibers.
Matthias Georg Steiger +8 more
doaj +1 more source