Results 81 to 90 of about 86,543 (295)

Affinity‐Tuned Albumin Hitchhiking Extends the Bioorthogonal Capture Window in Pretargeting Radiotheranostics

Advanced Healthcare Materials, EarlyView.
Pharmacokinetic engineering of clickable radioligands was achieved via albumin hitchhiking approach within a pretargeted radiotheranostics delivery platform. This approach reduced rapid renal elimination, expanded bioorthogonal capture window, boosted tumor uptake by ∼3.5‐fold, and improved the tumor‐to‐liver ratio by ∼6‐fold.
Xie He, Nicholas L. Fletcher, Gayathri R. Ediriweera, Yifei Zhu, James Humphries, Christopher B. Howard, Jiazheng Wang, Craig A. Bell, Kristian Kempe, Kristofer J. Thurecht   +9 more
wiley   +1 more source

Molecular cloning of the Na,K-ATPase α-subunit in developing brine shrimp and sequence comparison with higher organisms [PDF]

, 1989
We report here the molecular cloning, nucleotide sequence, and predicted amino acid sequence of an α-subunit of the developmentally useful model, Artemia.
Baxter-Lowe, Lee Ann, Lowell E. Hokin, Guo, Jian Zhong, Hokin, Lowell E., Lee Ann Baxter-Lowe, Ellen E. Bergstrom, Bergstrom, Ellen E., Jian Zhong Guo   +7 more
core   +1 more source

Conjugated Porphyrin Polymer With Superior Charge Transfer for Photodynamic‐Immunomodulatory Prevention of CIED Infection in Diabetes

Advanced Healthcare Materials, EarlyView.
Under NIR irradiation, PTCPP generates ROS to eliminate MRSA biofilms and promote M1 macrophage polarization for phagocytosis. After bacterial clearance, the material is phagocytosed by macrophages, shifting their polarization to M2 phenotype via metabolic reprogramming, which synergistically enhances anti‐infection therapy and wound healing.
Honghao Ma, Jinguo Xu, Rongrong Gu, Chuanhui Dong, Te Chen, Yi Mu, Jing Zhu, Yating Wang, Wen Wang, Yingying Zhang, Hongyu Ma, Xiaochen Wang, Sheng Ye, Cheng Cheng   +13 more
wiley   +1 more source

A Potential Food-Grade Cloning Vector for Streptococcus thermophilus That Uses Cadmium Resistance as the Selectable Marker [PDF]

, 2015
A potential food-grade cloning vector, pND919, was constructed and transformed into S. thermophilus ST3-1, a plasmid-free strain. The vector contains DNAs from two different food-approved organisms, Streptococcus thermophilus and Lactococcus lactis.
Su, Ping, Wong, Wing Yee, Liu, Chun-Qiang, Dunn, Noel W, Allison, Gwen   +4 more
core   +1 more source

Engineering a DYRK1B R102C mutation: insights into metabolic syndrome pathogenesis through lentiviral gene delivery [PDF]

Medical Laboratory Journal
Background: A rare heterozygous DYRK1B mutation (R102C) recently linked to a familial form of metabolic syndrome prompted this study to introduce the R102C mutation into the mouse DYRK1B gene, utilizing recombinant lentiviruses for long-term gene ...
Afrooz Daneshparvar, Iman Jamhiri, Vahid Razban, Jafar Fallahi, Nasrin Hamidizadeh, Behnam Moghtaderi, Mehdi Dianatpour   +6 more
doaj  

Lipid Nanoparticle Co‐Delivery of mRNA and a Small Molecule Drug for Oral Cancer Chemoimmunotherapy

Advanced Materials, EarlyView.
Co‐encapsulation of p53 mRNA and the small molecule ciclopirox within a lipid nanoparticle yields an all‐in‐one chemoimmunotherapy for oral squamous cell carcinoma. The platform engages caspase‐driven apoptosis in cancer cells while repolarizing tumor‐associated macrophages, achieving tumor reduction in both p53‐susceptible and p53‐resistant models and
Marshall S. Padilla, Jacqueline J. Li, Qunzhou Zhang, Korey Patwari, Shihong Shi, Hannah M. Yamagata, Ryann A. Joseph, Briyanna N. Hymms, Sridatta V. Teerdhala, Emily Fitzgerald, Ori Z. Chalom, Kushol Gupta, Mohamad‐Gabriel Alameh, Drew Weissman, Anh D. Le, Michael J. Mitchell   +15 more
wiley   +1 more source

Target Gene Inactivation in Cyanobacterium Anabaena sp. PCC 7120

Bio-Protocol, 2016
Anabaena sp. strain PCC 7120 has long served as a model organism for investigating N2-fixation, photosynthesis, and various plant-type metabolic pathways and biofuel production, as well as cellular differentiation (Xu et al., 2008, Halfmann et al., 2014,
Kangming Chen, Huilan Zhu, Liping Gu, Shengni Tian, Ruanbao Zhou   +4 more
doaj   +1 more source

Resolving Heterogeneity of Targeted Lipid Nanoparticles Through Solution‐Based Biophysical Analyses

Advanced Materials, EarlyView.
AF4‐UV‐DLS‐MALS‐SAXS resolves previously inaccessible targeted lipid nanoparticle (tLNP) subpopulations that differ in size, shape, and composition. Correlation of subpopulation‐resolved biophysical properties with in vivo RNA delivery reveals that targeted placental transfection is associated with distinct tLNP subpopulations rather than ensemble ...
Hannah C. Geisler, Hannah C. Safford, Ajay S. Thatte, Marshall S. Padilla, Elisa Battistini, Hannah M. Yamagata, Violet M. Ullman, Alex Chan, Benjamin E. Nachod, Anushka Agrawal, Maxwell B. Watkins, Jesse B. Hopkins, Andrew Tsourkas, Kushol Gupta, Michael J. Mitchell   +14 more
wiley   +1 more source

What is Cloning? [PDF]

This free resource from the University of Utah's Genetic Science Learning Center provides an overview of cloning for the layperson. The Genetic Science Learning Center "is a nationally and internationally-recognized education program that ...

core  

Parallel DNA pyrosequencing unveils new zebrafish microRNAs

BMC Genomics, 2009
Background MicroRNAs (miRNAs) are a new class of small RNAs of approximately 22 nucleotides in length that control eukaryotic gene expression by fine tuning mRNA translation.
Arrais Joel, Gomes Ana C, Egas Conceição, Santos Bruno, Pereira Patrícia M, Soares Ana R, Oliveira José L, Moura Gabriela R, Santos Manuel AS   +8 more
doaj   +1 more source