Results 81 to 90 of about 184,561 (308)

Amyloidogenic Peptide Fragments Designed From Bacterial Collagen‐like Proteins Form Hydrogel

Advanced Functional Materials, EarlyView.
This study identified amyloidogenic sequence motifs in bacterial collagen‐like proteins and exploited these to design peptides that self‐assemble into β‐sheet fibers and form hydrogels. One hydrogel supported healthy fibroblast growth, showing promise for biocompatible materials. Our work demonstrates that bacterial sequences can be harnessed to create
Vamika Sagar, Xuen J Ng, Lukas Lechel, Ashish Chand Rajwar, Hendrik Bargel, Abhishek A. Jalan   +5 more
wiley   +1 more source

Engineering a DYRK1B R102C mutation: insights into metabolic syndrome pathogenesis through lentiviral gene delivery [PDF]

Medical Laboratory Journal
Background: A rare heterozygous DYRK1B mutation (R102C) recently linked to a familial form of metabolic syndrome prompted this study to introduce the R102C mutation into the mouse DYRK1B gene, utilizing recombinant lentiviruses for long-term gene ...
Afrooz Daneshparvar, Iman Jamhiri, Vahid Razban, Jafar Fallahi, Nasrin Hamidizadeh, Behnam Moghtaderi, Mehdi Dianatpour   +6 more
doaj  

The Terminal Domains of Collagen‐Like Silk Direct Molecular Interactions and Liquid–Liquid Phase Separation‐Mediated Material Assembly

Advanced Functional Materials, EarlyView.
Terminal domains of recombinant collagen‐like silk regulate hierarchical self‐assembly across molecular and material scales. We show that terminal domains of collagen‐like silk enhance triple‐helix stability and drive liquid–liquid phase separation, enabling reversible formation of fibers and self‐healing films.
Mengjie Shen, Daniil Astapov, Nur Alia Oktaviani, Mami Goto, Keiji Numata, A. Sesilja Aranko   +5 more
wiley   +1 more source

Target Gene Inactivation in Cyanobacterium Anabaena sp. PCC 7120

Bio-Protocol, 2016
Anabaena sp. strain PCC 7120 has long served as a model organism for investigating N2-fixation, photosynthesis, and various plant-type metabolic pathways and biofuel production, as well as cellular differentiation (Xu et al., 2008, Halfmann et al., 2014,
Kangming Chen, Huilan Zhu, Liping Gu, Shengni Tian, Ruanbao Zhou   +4 more
doaj   +1 more source

Cloning of the pig renal organic anion transporter 1 (pOAT1)

Biochimie, 2002
A pig kidney cDNA library was screened for the porcine ortholog of the multispecific organic anion transporter 1 (pOAT1). Several positive clones were isolated resulting in two alternatively spliced cDNA clones of pOAT1 (pOAT1 and pOAT1A). pOAT1-cDNAs consist of 2126 or 1895 base pairs (EMBL Acc. No.
Hagos, Yohannes, Bahn, A., Asif, Abdul Rahman, Krick, W., Sendler, M., Burckhardt, Gerhard   +5 more
openaire   +3 more sources

Mapping Nanoscale Protein‐Corona Kinetics of DoE‐Optimized Perfluorocarbon Encapsulated‐PLGA Nanoparticles by In Situ, Time‐Resolved Synchrotron SAXS

Advanced Healthcare Materials, EarlyView.
A two‐phase workflow (OFAT screening followed by central composite design) maps how processing variables tune PFCE‐PLGA nanoparticle size, dispersity, surface charge, loading, and 19F‐MRI signal. In situ, time‐resolved synchrotron SAXS tracks albumin‐corona growth on intact dispersions and reveals PFCE‐dependent adsorption pathways.
Joice Maria Joseph, Maria Rosa Gigliobianco, Cristina Minnelli, Paolo Moretti, Francesco Spinozzi, Giovanna Mobbili, Sara Pistolesi, Cristina Casadidio, Gabriella Teti, Roberta Censi, Stefano Antonio Gattone, Piera Di Martino   +11 more
wiley   +1 more source

Parallel DNA pyrosequencing unveils new zebrafish microRNAs

BMC Genomics, 2009
Background MicroRNAs (miRNAs) are a new class of small RNAs of approximately 22 nucleotides in length that control eukaryotic gene expression by fine tuning mRNA translation.
Arrais Joel, Gomes Ana C, Egas Conceição, Santos Bruno, Pereira Patrícia M, Soares Ana R, Oliveira José L, Moura Gabriela R, Santos Manuel AS   +8 more
doaj   +1 more source

Novel applications of shotgun phage display [PDF]

, 2004
In a shotgun phage display library, theoretically, the entire proteome of a bacterium is represented. Phages displaying specific polypeptides can be isolated by affinity selection, while the corresponding gene remains physically linked to the gene ...
Rosander, Anna
core  

Nitrogen regulation of protein–protein interactions and transcript levels of GlnK PII regulator and AmtB ammonium transporter homologs in Archaea [PDF]

, 2013
Gene homologs of GlnK PII regulators and AmtB-type ammonium transporters are often paired on prokaryotic genomes, suggesting these proteins share an ancient functional relationship.
Adler, Allers, Andrade, Atkinson, Bell, Bitan-Banin, Bonete, Camacho, Chellamuthu, Cline, Conroy, Coutts, Detsch, Dodsworth, Eichler, Garber, Gruswitz, Hammelmann, Han, Hervás, Huergo, Huergo, Huergo, Javelle, Javelle, Jiang, Khademi, Lee, Liu, Maier, Mangum, Martínez-Espinosa, Martínez-Espinosa, Moure, Najera-Fernandez, Pedro-Roig, Pedro-Roig, Pedro-Roig, Radchenko, Rodriguez-Valera, Russek, Sant'Anna, Severi, Strösser, Thomas, Thornton, Tremblay, Weiss, Werner, Xu, Zamaroczy, Zhang, Zhang, Zheng   +53 more
core   +2 more sources

Aptamer‐Based Delivery Systems for VEGF and NGF Modulation in Ocular Therapies

Advanced Healthcare Materials, EarlyView.
Aptamer‐based delivery systems targeting NGF and VEGF hold significant potential for precise and sustained treatment of complex diseases affecting both the anterior and posterior segments of the eye. This review critically summarizes current strategies for NGF and VEGF delivery as well as VEGF sequestration, providing a clinical perspective on how ...
Nadine Best, Gizem Karabiyik, Gerd Geerling, Sarah Barbara Zwingelberg, Gregor Lang, Martin Humenik   +5 more
wiley   +1 more source