Results 261 to 270 of about 889,258 (299)
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2004
Expression cloning involves the selection of specific polypeptides, generated from a cDNA or genomic DNA library, based on certain characteristics of the expressed proteins, such as antibody or ligand binding, recognition by T-cells, function, or complementation of cell defects.
Michael J, Lodes +3 more
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Expression cloning involves the selection of specific polypeptides, generated from a cDNA or genomic DNA library, based on certain characteristics of the expressed proteins, such as antibody or ligand binding, recognition by T-cells, function, or complementation of cell defects.
Michael J, Lodes +3 more
openaire +2 more sources
Science, 2017
Overgrowth of mutant blood cells is common in older people—and a risk factor for serious ...
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Overgrowth of mutant blood cells is common in older people—and a risk factor for serious ...
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2013
This protocol describes the basic steps involved in conventional plasmid-based cloning. The goals are to insert a DNA fragment of interest into a receiving vector plasmid, transform the plasmid into E. coli, recover the plasmid DNA, and check for correct insertion events.
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This protocol describes the basic steps involved in conventional plasmid-based cloning. The goals are to insert a DNA fragment of interest into a receiving vector plasmid, transform the plasmid into E. coli, recover the plasmid DNA, and check for correct insertion events.
openaire +2 more sources
2009
Reverse chemical genomic approach is expected to greatly expedite the discovery of new compounds, which modulate biological phenotypes in various ways. However, toward this end, various contents and platforms must be well prepared in the research community.
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Reverse chemical genomic approach is expected to greatly expedite the discovery of new compounds, which modulate biological phenotypes in various ways. However, toward this end, various contents and platforms must be well prepared in the research community.
openaire +2 more sources
Isolating live cell clones from barcoded populations using CRISPRa-inducible reporters
Nature Biotechnology, 2021Christian Umkehrer +2 more
exaly