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Fish & Shellfish Immunology, 2020
Transcription factor c-Jun is a member of AP-1 transcription complex that can be induced by various pathogens and plays a broad regulatory role in vertebrate immune response. In this study, the complete c-Jun cDNA of large yellow croaker Larimichthys crocea (Lcc-Jun) was cloned, whose open reading frame (ORF) is 984 bp long and encodes a protein of 327
Libing Xu +4 more
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Transcription factor c-Jun is a member of AP-1 transcription complex that can be induced by various pathogens and plays a broad regulatory role in vertebrate immune response. In this study, the complete c-Jun cDNA of large yellow croaker Larimichthys crocea (Lcc-Jun) was cloned, whose open reading frame (ORF) is 984 bp long and encodes a protein of 327
Libing Xu +4 more
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Cloning Retroviruses: Retrovirus Cloning?
1981The retroviridae are viruses that possess the unique ability to transcribe their genomic RNA into a DNA copy through a process called reverse transcription (1,2) (Figure 1). In infected cells, the DNA intermediates (unintegrated provirus) become stably associated with the host chromosome via an as yet uncharacterized integration process to form the ...
William L. McClements +1 more
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Journal of Immunology, 1987
We have previously shown that at least two types of Lyt-1+, Lyt-2-, L3T4+ helper T cell clones can be distinguished in vitro by different patterns of lymphokine secretion and by different forms of B cell help.
D. Cher, T. Mosmann
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We have previously shown that at least two types of Lyt-1+, Lyt-2-, L3T4+ helper T cell clones can be distinguished in vitro by different patterns of lymphokine secretion and by different forms of B cell help.
D. Cher, T. Mosmann
semanticscholar +1 more source
Veterinary Clinics of North America: Equine Practice, 2006
Equine cloning is now in use as a clinical technique. It is available commercially, and its efficiency seems to be increasing. The foals produced by cloning may differ in some phenotypic and behavioral traits from the original animal but should produce offspring that reflect those that the original donor animal would have produced.
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Equine cloning is now in use as a clinical technique. It is available commercially, and its efficiency seems to be increasing. The foals produced by cloning may differ in some phenotypic and behavioral traits from the original animal but should produce offspring that reflect those that the original donor animal would have produced.
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2000
Abstract If there is one respect in which cancer is special as an illness, it is that all the cancer cells in one patient are, in almost every case, derived from a single cell. They are a clone. Not necessarily all identical because the capacity of the progeny of the founder or mother cell to diversify genetically is another critical ...
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Abstract If there is one respect in which cancer is special as an illness, it is that all the cancer cells in one patient are, in almost every case, derived from a single cell. They are a clone. Not necessarily all identical because the capacity of the progeny of the founder or mother cell to diversify genetically is another critical ...
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Cloning and Stem Cells, 2003
Over the past six years, hundreds of apparently normal calves have been cloned worldwide from bovine somatic donor cells. However, these surviving animals represent less than 5% of all cloned embryos transferred into recipient cows. Most of the remaining 95% die at various stages of development from a predictable pattern of placental and fetal ...
B, Oback, D N, Wells
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Over the past six years, hundreds of apparently normal calves have been cloned worldwide from bovine somatic donor cells. However, these surviving animals represent less than 5% of all cloned embryos transferred into recipient cows. Most of the remaining 95% die at various stages of development from a predictable pattern of placental and fetal ...
B, Oback, D N, Wells
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2013
Many methods have been developed for the cloning of PCR products. These methods include blunt-end cloning, TA cloning, and using restriction sites incorporated into the PCR primers. The restrictionless cloning technique allows efficient directional cloning of PCR products into any cloning site within a vector regardless of whether the sites are ...
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Many methods have been developed for the cloning of PCR products. These methods include blunt-end cloning, TA cloning, and using restriction sites incorporated into the PCR primers. The restrictionless cloning technique allows efficient directional cloning of PCR products into any cloning site within a vector regardless of whether the sites are ...
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Cloning in Plasmid Vectors: Directional Cloning
Cold Spring Harbor Protocols, 2020This protocol describes the standard, old-fashioned but reliable procedure for cloning linear DNA fragments whose ends are incompatible with each other but are compatible with those of the linearized vector.
Michael R, Green, Joseph, Sambrook
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Nature, 1997
What are the implications for humankind of the astounding report two weeks ago of the production of viable sheep from adult cells? The moral imperative of preserving human dignity must remain paramount.
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What are the implications for humankind of the astounding report two weeks ago of the production of viable sheep from adult cells? The moral imperative of preserving human dignity must remain paramount.
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2013
One major obstacle in realizing the potential behind human embryonic stem cells (hESC) is the availability of efficient and reliable engineering methods. Such methods require cloning technologies that can be applied to a variety of platforms and can serve multiple functions.
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One major obstacle in realizing the potential behind human embryonic stem cells (hESC) is the availability of efficient and reliable engineering methods. Such methods require cloning technologies that can be applied to a variety of platforms and can serve multiple functions.
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