Results 331 to 340 of about 1,771,671 (365)

High-throughput cloning of Campylobacter jejuni ORfs by in vivo recombination in Escherichia coli.

Journal of Proteome Research, 2004
A rate-limiting and costly step in many proteomics analyses is the cloning of all of the ORFs for an organism into technique-specific vectors. Here, we describe the generation of a Campylobacter jejuni expression clone set using a high-throughput cloning
J. Parrish, T. Limjindaporn, Julie A. Hines, Jiayou Liu, Guozhen Liu, R. Finley   +5 more
semanticscholar   +1 more source

Cloning and Functional Expression of a Human Liver Organic Cation Transporter

Molecular Pharmacology, 1997
Polyspecific organic cation transporters in the liver mediate the elimination of a wide array of endogenous amines and xenobiotics. In contrast to our understanding of the mechanisms of organic cation transport in rat liver, little is known about the mechanisms of organic cation transport in the human liver.
Andrew T. Gray, Kathleen M. Giacomini, Mark J. Dresser, Spencer C. Yost, Shigeyuki Terashita, Lei Zhang   +5 more
openaire   +3 more sources

Genetic organization and cloning of kirsten murine sarcoma virus DNA

Biochemical and Biophysical Research Communications, 1982
Summary The Kirsten murine sarcoma virus genome is a recombinant molecule derived from the Kirsten murine leukemia virus genome and sequences transduced from a normal rat cell. We show here that leukemia virus derived sequences comprise the 5′ terminal 0.5 kilobasepairs (kbp) and the 3′ terminal 1.2 kbp of the linear 6.5 kbp sarcoma virus DNA.
Roger J. Avery, John Norton
openaire   +3 more sources

Genomic organization and cloning of the human homologue of murine Sipa-1

Gene, 1998
Murine Sipa-1 (signal-induced proliferation associated protein) is a mitogen induced GTPase activating protein (GAP). While mapping candidate genes for multiple endocrine neoplasia type 1 (MEN1) at 11q13, we cloned the human homologue of Sipa-1. Herein, we report the complete cDNA sequence, expression, and genomic organization of SIPA-1.
Mark P. Sawicki, Eric Wang, Elizabeth Arnold, Nitin Udar, Kent W. Small, David Burbee, Sam A. Ebrahimi   +6 more
openaire   +3 more sources

Cloning and functional analysis of alkB genes in Alcanivorax borkumensis SK2.

Environmental Microbiology, 2004
Alcanivorax is an alkane-degrading marine bacterium which propagates and becomes predominant in crude-oil-containing seawater when nitrogen and phosphorus nutrients are supplemented.
A. Hara, S. Baik, K. Syutsubo, N. Misawa, T. H. Smits, J. B. van Beilen, S. Harayama   +6 more
semanticscholar   +1 more source

Molecular and Functional Characteristics of Cloned Human Organic Cation Transporters [PDF]

, 2005
Within the last 5 years the organic cation transport field has moved from tissue and cellular levels to a molecular level of investigation. As we gain a better understanding of the genes and proteins responsible for the multiple mechanisms of organic cation transport, we can begin to apply this knowledge to pharmaceutical and drug discovery research ...
Kathleen M. Giacomini, Mark J. Dresser, Lei Zhang   +2 more
openaire   +2 more sources

Cloning and characterization of a hemolysin gene from Actinobacillus (Haemophilus) pleuropneumoniae.

DNA, 1989
Neutralizing antisera to the leukotoxin secreted by Pasteurella haemolytica neutralized the hemolysin of Actinobacillus pleuropneumoniae and recognized a 110-kD antigen in cell-free culture supernatants from this organism.
Yungfu Chang, R. Young, D. Struck
semanticscholar   +1 more source

Cloning of Aged Animals A Medical Model for Tissue and Organ Regeneration

Trends in Cardiovascular Medicine, 2001
Cloning by nuclear transfer has great potential application in pharmaceutical protein production, xeno-transplantation, and perhaps most excitingly, therapeutic cloning. In therapeutic cloning a patient's own skin cells can be used to generate cloned embryos from which embryonic stem cells are isolated.
X.C Tian, Xiangzhong Yang, Chikara Kubota   +2 more
openaire   +2 more sources

Toward cloning and mapping the genome of Drosophila.

Science, 1991
An ultimate goal of Drosophila genetics is to identify and define the functions of all the genes in the organism. Traditional approaches based on the isolation of mutant genes have been extraordinary fruitful.
John R. Merriam, Michael Ashburner, Daniel L. Hartl, F. C. Kafatos   +3 more
semanticscholar   +1 more source

Cloning, genomic organization, and tissue-specific expression of the RASL11B gene

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 2007
RASL11B is a member of the small GTPase protein family with a high degree of similarity to RAS proteins. Cloning of RASL11B mRNA and in silico analyses revealed that the human RASL11B gene spans about 4.5 kb and comprises four exons on chromosomal locus 4q12.
Georg Fuellen, Georg Fuellen, Michael Schnoor, Paul Cullen, Michael Spitzer, Michael Spitzer, Katrin Stolle, Stefan Lorkowski   +7 more
openaire   +3 more sources