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THE FORMATION OF METHYLGLYOXAL BY CLOSTRIDIUM ACETOBUTYLICUM
L.B. Pett, A.M. Wynne
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Mutagenesis of Clostridium acetobutylicum
Journal of Applied Bacteriology, 1985Mutagenesis of the obligate anaerobe Clostridium acetobutylicum was best accomplished using agents (e.g. ethyl methane sulphonate or N ‐methyl‐ N '‐nitro‐ N ...
S N, Bowring, J G, Morris
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Engineering Clostridium acetobutylicum for alcohol production
Journal of Biotechnology, 2013While Clostridium acetobutylicum has been used for large-scale butanol production (ABE fermentation), its by-product acetone cannot be used as a biofuel. In this study, C. acetobutylicum was engineered for alcohol titers (butanol plus ethanol). The adc gene was inactivated to eliminate acetone production, and glutathione biosynthetic capability was ...
Hou, Xiaohu +6 more
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Transfer of Tn1545 and Tn916 to Clostridium acetobutylicum
Plasmid, 1989Tn1545, a conjugative transposon originally discovered in Streptococcus pneumoniae, has been transferred from Enterococcus faecalis and Bacillus subtilis to Clostridium acetobutylicum NCIB 8052. Transfer between different strains of C. acetobutylicum has also been observed. Insertion of Tn1545 into the C.
R C, Woolley +4 more
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Initiation of endospore formation in Clostridium acetobutylicum
Anaerobe, 2004Endospore formation in bacilli and clostridia shows remarkable similarities in morphology as well as in physiological and molecular biological cellular events. Major differences are the formation of clostridial stage cells and granulose accumulation in clostridia. In both genera, a cascade of sigma factors is activated after septation (by help of sigma(
Peter, Dürre, Concha, Hollergschwandner
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Biotechnology Advances, 2022
Solventogenic clostridia are not a strictly defined group within the genus Clostridium but its representatives share some common features, i.e. they are anaerobic, non-pathogenic, non-toxinogenic and endospore forming bacteria. Their main metabolite is typically 1-butanol but depending on species and culture conditions, they can form other metabolites ...
Petra, Patakova +6 more
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Solventogenic clostridia are not a strictly defined group within the genus Clostridium but its representatives share some common features, i.e. they are anaerobic, non-pathogenic, non-toxinogenic and endospore forming bacteria. Their main metabolite is typically 1-butanol but depending on species and culture conditions, they can form other metabolites ...
Petra, Patakova +6 more
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Pyruvate fermentation by Clostridium acetobutylicum
Biochemistry and Cell Biology, 1989Clostridium acetobutylicum ATCC 824 using pyruvate as the sole carbon source produced mainly acetate and butyrate as end products of fermentation. Acetate and butyrate kinase activities were higher in cells growing in the presence of pyruvate than glucose, whereas the level of the acetoacetate decarboxylase, an enzyme involved in solvent formation ...
R, Janati-Idrissi +4 more
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Engineering Cellulase Activity into Clostridium acetobutylicum
2012Clostridium acetobutylicum produces substantial amounts of butanol, and an engineered cellulolytic strain of the bacterium would be an attractive candidate for biofuel production using consolidated bioprocessing. Recent studies have shown that this solventogenic bacterium can be used as a host for heterologous production and secretion of individual ...
Henri-Pierre, Fierobe +2 more
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Fermentation of xylan by Clostridium acetobutylicum
Enzyme and Microbial Technology, 1986Abstract The ability of Clostridium acetobutylicum to utilize hemicelluloses as a substrate for butanol production was examined. Direct fermentation of byproduct corn fibres from the wet milling process showed complete utilization of starch and 20% utilization of hemicellulose with concurrent production of solvents and organic acids. Fermentations of
S.A. Lemmel +2 more
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Vectors for Use in Clostridium acetobutylicum
1993In the last two decades, interest in using surplus/waste biomass in biocatalytic processes to produce chemicals and fuels of high added value, as an alternative to chemical synthesis from petroleum feedstocks, has exhibited considerable fluctuation. The underlying argument for such developments seems irrefutable. Fossil fuels are a finite resource.
Nigel P. Minton +4 more
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