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Experimental Clostridium perfringens Type D Enterotoxemia in Goats
Veterinary Pathology, 1998The effects of intraduodenal administration of Clostridium perfringens cultures and culture products in goats were evaluated to develop a reliable experimental model of enterotoxemia in this species. Five conventionally reared, 11–16-week-old Angora goat kids were dosed intraduodenally with whole cultures of C. perfringens type D; five similar animals
Uzal, F. A., Kelly, W. R.
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EFFECT OF CARBOHYDRATES ON TOXINOGENESIS BY CLOSTRIDIUM PERFRINGENS TYPE D
Canadian Journal of Microbiology, 1965Glucose and dextrin were compared for their ability to support toxinogenesis in cultures of strain D7 of Clostridium perfringens type D with pH controlled at 7.0 or uncontrolled. At pH 7.0 both carbohydrates supported toxinogenesis equally: the yields of alpha, epsilon, kappa, and theta toxins were 20 MLD, 70,000 to 80,000 MLD, 400 to 500 arbitrary ...
A H, HAUSCHILD, H, PIVNICK
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Production and purification of Clostridium perfringens type D epsilon toxin and IgY antitoxin
Anaerobe, 2021The aim of this study was to purify Clostridium perfringens type D epsilon toxin and produce and purify anti-epsilon chicken immunoglobulin Y (IgY). A single-step ion exchange chromatography resulted in a high-yield and high-purity toxin, while ion exchange chromatography followed by gel filtration resulted in the highest purity of the toxin, but at a ...
Guilherme Guerra Alves +6 more
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EFFECT OF CONTROLLED pH ON TOXINOGENESIS BY CLOSTRIDIUM PERFRINGENS TYPE D
Canadian Journal of Microbiology, 1965A strain of Clostridium perfringens type D was grown in a complex medium with or without pH control. Rate and extent of growth was the same without pH control and at controlled pH levels between 6.0 and 7.4, but was reduced at pH 7.6. With controlled pH at optimum levels the yields of alpha, epsilon, kappa, and theta toxins were 2, 6, 2, and 8 times ...
H, PIVNICK +3 more
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Separation of Clostridium Perfringens Type D Toxins
The Journal of Immunology, 1965Summary A method was developed for the complete separation of Clostridium perfringens type D toxins. ε and θ toxins were separated from each other and from the remaining toxins on a single column of diethylaminoethyl cellulose.
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Studies on ϵ-prototoxin of Clostridium perfringens type D
Archives of Biochemistry and Biophysics, 1964Purified prototoxin of Clostridium perfringens type D was separated by column chromatography on CM-cellulose or DEAE-cellulose into two and three fractions respectively. The fractions exhibited prototoxin activity and showed immunochemical identity. The purified prototoxin gave a single component in the ultracentrifuge with a sedimentation constant of ...
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Clostridium perfringens type D enterotoxaemia in neonatal lambs
Veterinary Record, 2007SIR, — Ovine enterotoxaemia caused by Clostridium perfringens type D occurs most commonly in lambs at four to 10 weeks of age and in finishing lambs of six months and older, often associated with increased amounts of dietary carbohydrate ([Lewis 2000][1]).
S. F. E. Scholes +4 more
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Clostridium perfringens toxins (type A, B, C, D, E)
Pharmacology & Therapeutics, 1980Abstract Clostridum perfringens types A,B,C,D, and E produce at least 12 different antigens, referred to as toxins , that may be involved in pathogenesis . These antigens have been given the names alpha, beta, epsilon , and iota toxin (‘major’ toxins), and delta, theta, kappa (collagenase), lambda (protease), mu (hyaluronidase), nu ...
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Neurological disorders produced by Clostridium perfringens type D epsilon toxin
Anaerobe, 2004Although the epsilon toxin of Clostridium perfringens type D produces disease in many species of domestic livestock, neurological disturbance is more common and better studied in sheep. High levels of circulating toxin, especially in lambs, cause cerebral microvascular endothelial damage with disruption of the blood-brain barrier leading to a severe ...
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