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Endoglucanase from Clostridium thermocellum

1988
Publisher Summary Clostridium thermocellum produces several endoglucanases that are part of a cellulase complex termed cellulosome.1 Five of the endoglucanases have been purified and characterized. Two of these endoglucanases from C. thermocellum are produced by Escherichia coli strains carrying the genes that encode for the proteins.
Thomas K. Ng, J.G. Zeikus
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Cellulosomes from Clostridium thermocellum

1988
Publisher Summary Biomass in the form of cellulose is the major constituent of plant matter, thereby comprising the earth's most abundant natural organic resource. Although the repeating unit of this biopolymer is a simple disaccharide (cellobiose), cellulose fibrils are organized into a complicated paracrystalline state.
Raphael Lamed, Edward A. Bayer
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Studies on cellulase production by Clostridium thermocellum

European Journal of Applied Microbiology and Biotechnology, 1980
Clostridium thermocellum ATCC 27405 (and its improved cellulase-producing mutant, AS-39) is an anaerobic thermophile that produces endo-β-glucanase and exo-β-glucanase when grown on cellobiose or cellulose as major carbon source (Shinmyo et al. 1979). The site of cellulase accumulation was at least 95% extracellular.
D. V. Garcia-Martinez   +3 more
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Identification of the endoglucanase encoded by the celB gene of Clostridium thermocellum

Biochimie, 1983
The endoglucanase encoded by the celB gene of Clostridium thermocellum was purified from an E. coli strain carrying and expressing the C. thermocellum gene cloned in the plasmid pBR322. The preparation showed two active bands, with Mr 55,000 and 53,000, presumably derived from the primary translation product by proteolysis.
P, Beguin, P, Cornet, J, Millet
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The cellulolytic enzyme complex of Clostridium thermocellum is very large

Biochemical and Biophysical Research Communications, 1985
The cellulolytic enzyme system bound to cellulose during the early stages of growth of C. thermocellum on this substrate was resolved into two major complexes. These complexes, as viewed by electron microscopy, are spherical particles with diameters of 210 A and 610 A and calculated molecular weights of 4.2 million and 102 million daltons, respectively.
M P, Coughlan   +5 more
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Transcriptome data for Clostridium thermocellum

Transcriptome data for rare earth-treated Clostridium thermocellum and untreated.
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The cellulosome of Clostridium thermocellum

Biochemical Society Transactions, 1998
P, Béguin, P M, Alzari
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The chaperonins of Clostridium thermocellum

Biochemical Society Transactions, 1995
S J, Cross, R B, Freedman
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Clostridium thermocellum

2015
Steven D. Brown   +3 more
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Xylan-breakdown apparatus of Clostridium thermocellum

Cellulose, 2022
Pedro R. V. Hamann, Eliane F. Noronha
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