Novel Function of Ct Xyn5A from Acetivibrio thermocellus : Dual Arabinoxylanase and Feruloyl Esterase Activity in the Same Active Site** [PDF]
ChemBioChem, 2023 Same site, different outcome: A novel feruloyl esterase functionality of arabinoxylanase CtXyn5A from A. thermocellus has been discovered. The enzyme catalyses both types of reaction in a sequential fashion in the same active site. As its esterase activity is higher on carbohydrate polymers than that of a designated ferulic acid esterase, we conclude ...
Savvina Leontakianakou
exaly +6 more sources
Structure-Based Engineering to Improve Thermostability of Acetivibrio thermocellus AtBgl1A β-Glucosidase [PDF]
ACS Omegaβ-Glucosidases are essential enzymes in cellulose degradation and hold significant promise for industrial applications, particularly in biorefinery processes. This study focused on the structural and functional characterization of AtBgl1A, a glycoside hydrolase family 1 β-glucosidase from Acetivibrio thermocellus, and its rational engineering to ...
Kiattawee Choowongkomon +2 more
exaly +5 more sources
Synergy of Cellulase Systems between Acetivibrio thermocellus and Thermoclostridium stercorarium in Consolidated-Bioprocessing for Cellulosic Ethanol [PDF]
Microorganisms, 2022 Anaerobes harbor some of the most efficient biological machinery for cellulose degradation, especially thermophilic bacteria, such as Acetivibrio thermocellus and Thermoclostridium stercorarium, which play a fundamental role in transferring ...
Na Wang +4 more
doaj +5 more sources
Cellulolytic and hemicellulolytic capacity of Acetivibrio clariflavus [PDF]
Applied Microbiology and BiotechnologyLignocellulosic biomass represents the largest available renewable source of carbon. It is a recalcitrant mixture of different polysaccharides and lignin.
Katarina Suchová, Vladimir Puchart
exaly +3 more sources
Discovery and Biosynthesis of Celluxanthenes, Antibacterial Arylpolyene Alkaloids From Diverse Cellulose-Degrading Anaerobic Bacteria. [PDF]
Angew Chem Int Ed EnglThe structure and biosynthetic origin of the enigmatic “yellow affinity substance” (YAS) from the anaerobe thermophile Clostridium thermocellum (now: Acetivibrio thermocellus) were elucidated. YAS is a complex of unusual antibacterial arylpolyene alkaloids (celluxanthenes) that are produced by various cellulolytic anaerobes.
Ishida K +9 more
europepmc +3 more sources
Functional and structural characterization of AtAbf43C: an exo-1,5-α-L-arabinofuranosidase from Acetivibrio thermocellus DSM1313. [PDF]
Biochem JAcetivibrio thermocellus degrades diverse polysaccharides found in plant biomass using an array of glycoside hydrolase (GH) enzymes. Here, we describe the structure and function of AtAbf43C, an uncharacterized GH family 43 subfamily 26 (GH43_26) α-L-arabinofuranosidase (EC 3.2.1.55) from A. thermocellus. AtAbf43C is optimally active on para-nitrophenol-
Galindo JL +4 more
europepmc +4 more sources
Enhanced Activity by Genetic Complementarity: Heterologous Secretion of Clostridial Cellulases by Bacillus licheniformis and Bacillus velezensis [PDF]
Molecules, 2021 To adapt to various ecological niches, the members of genus Bacillus display a wide spectrum of glycoside hydrolases (GH) responsible for the hydrolysis of cellulose and lignocellulose. Being abundant and renewable, cellulose-containing plant biomass may
Alexander Arsov +2 more
doaj +2 more sources
Engineering Clostridium thermocellum for production of 2,3-butanediol from cellulose [PDF]
Metabolic Engineering CommunicationsClostridium thermocellum is a promising host for consolidated bioprocessing due to its ability to directly ferment cellulose into fuels and chemicals. However, natural product formation in this organism is limited.
S. Bilal Jilani +4 more
doaj +2 more sources
Genetic and metabolic drivers of membrane remodeling in Clostridium thermocellum under alcohol stress [PDF]
mSystemsClostridium thermocellum is a leading candidate for consolidated bioprocessing of lignocellulosic biomass into biofuels due to its native cellulolytic capabilities. Beyond ethanol, C.
Eashant Thusoo +6 more
doaj +2 more sources
Metabolic EngineeringAcetivibrio thermocellus (formerly Clostridium thermocellum) is a potential platform for lignocellulosic ethanol production. Its industrial application is hampered by low product titres, resulting from a low thermodynamic driving force of its central metabolism.
Antonius Van Maris
exaly +3 more sources