Novel Function of CtXyn5A From Acetivibrio Thermocellus: Dual Arabinoxylanase and Feruloyl Esterase Activity in the Same Active Site [PDF]
ChemBioChem, 2023 Same site, different outcome: A novel feruloyl esterase functionality of arabinoxylanase CtXyn5A from A. thermocellus has been discovered. The enzyme catalyses both types of reaction in a sequential fashion in the same active site. As its esterase activity is higher on carbohydrate polymers than that of a designated ferulic acid esterase, we conclude ...
Javier A Linares-Pastén +2 more
exaly +10 more sources
Synergy of Cellulase Systems between Acetivibrio thermocellus and Thermoclostridium stercorarium in Consolidated-Bioprocessing for Cellulosic Ethanol [PDF]
Microorganisms, 2022 Anaerobes harbor some of the most efficient biological machinery for cellulose degradation, especially thermophilic bacteria, such as Acetivibrio thermocellus and Thermoclostridium stercorarium, which play a fundamental role in transferring ...
Na Wang +4 more
doaj +6 more sources
Structure-Based Engineering to Improve Thermostability of Acetivibrio Thermocellus AtBgl1A β-Glucosidase [PDF]
ACS Omegaβ-Glucosidases are essential enzymes in cellulose degradation and hold significant promise for industrial applications, particularly in biorefinery processes. This study focused on the structural and functional characterization of AtBgl1A, a glycoside hydrolase family 1 β-glucosidase from Acetivibrio thermocellus, and its rational engineering to ...
Kiattawee Choowongkomon +2 more
exaly +5 more sources
Discovery and Biosynthesis of Celluxanthenes, Antibacterial Arylpolyene Alkaloids From Diverse Cellulose-Degrading Anaerobic Bacteria. [PDF]
Angew Chem Int Ed EnglThe structure and biosynthetic origin of the enigmatic “yellow affinity substance” (YAS) from the anaerobe thermophile Clostridium thermocellum (now: Acetivibrio thermocellus) were elucidated. YAS is a complex of unusual antibacterial arylpolyene alkaloids (celluxanthenes) that are produced by various cellulolytic anaerobes.
Ishida K +9 more
europepmc +5 more sources
Enhanced Activity by Genetic Complementarity: Heterologous Secretion of Clostridial Cellulases by Bacillus licheniformis and Bacillus velezensis [PDF]
Molecules, 2021 To adapt to various ecological niches, the members of genus Bacillus display a wide spectrum of glycoside hydrolases (GH) responsible for the hydrolysis of cellulose and lignocellulose. Being abundant and renewable, cellulose-containing plant biomass may
Alexander Arsov +2 more
doaj +3 more sources
Functional and Structural Characterization of AtAbf43C: An Exo-1,5-α-L-Arabinofuranosidase From Acetivibrio Thermocellus DSM1313 [PDF]
Biochemical JournalAcetivibrio thermocellus degrades diverse polysaccharides found in plant biomass using an array of glycoside hydrolase (GH) enzymes. Here, we describe the structure and function of AtAbf43C, an uncharacterized GH family 43 subfamily 26 (GH43_26) α-L-arabinofuranosidase (EC 3.2.1.55) from A. thermocellus. AtAbf43C is optimally active on para-nitrophenol-
Link James +2 more
exaly +5 more sources
Cellulolytic and Hemicellulolytic Capacity of Acetivibrio Clariflavus [PDF]
Applied Microbiology and BiotechnologyLignocellulosic biomass represents the largest available renewable source of carbon. It is a recalcitrant mixture of different polysaccharides and lignin.
Katarína Šuchová, Vladimir Puchart
exaly +3 more sources
Cellulosic Ethanol Production Through Cocultivation of Thermoanaerobacter brockii subsp. finnii DSM 3389 and Acetivibrio thermocellus JYT01 [PDF]
MicroorganismsConsolidated bioprocessing integrates enzyme production, cellulose hydrolysis, and fermentation in a single reactor; however, few naturally occurring microorganisms combine efficient cellulose degradation, broad sugar utilization, inhibitor tolerance ...
Zili Qiu, Chenggang Xu, Zhenxing Ren
doaj +2 more sources
Engineering Clostridium thermocellum for production of 2,3-butanediol from cellulose [PDF]
Metabolic Engineering CommunicationsClostridium thermocellum is a promising host for consolidated bioprocessing due to its ability to directly ferment cellulose into fuels and chemicals. However, natural product formation in this organism is limited.
S. Bilal Jilani +4 more
doaj +2 more sources
Integrative SAXS and AFM analysis of engineered carbohydrate-active enzyme assemblies with tunable spatial organization. [PDF]
Protein SciAbstract Cellulosomes are multi‐enzyme assemblies whose catalytic efficiency depends on the spatial organization of their components. However, their pronounced conformational flexibility has precluded quantitative characterization of inter‐enzyme distances and overall topology.
Pardo Larrabeiti I +11 more
europepmc +2 more sources