Results 11 to 20 of about 368 (86)

Pyrophosphate-free glycolysis in Clostridium thermocellum increases both thermodynamic driving force and ethanol titers [PDF]

Biotechnology for Biofuels and Bioproducts
Background Clostridium thermocellum is a promising candidate for production of cellulosic biofuels, however, its final product titer is too low for commercial application, and this may be due to thermodynamic limitations in glycolysis.
Bishal Dev Sharma, Shuen Hon, Eashant Thusoo, David M. Stevenson, Daniel Amador-Noguez, Adam M. Guss, Lee R. Lynd, Daniel G. Olson   +7 more
doaj   +2 more sources

Ethanol tolerance of Clostridium thermocellum: the role of chaotropicity, temperature and pathway thermodynamics on growth and fermentative capacity

Microbial Cell Factories, 2022
Background Clostridium thermocellum is a promising candidate for consolidated bioprocessing of lignocellulosic biomass to ethanol. The low ethanol tolerance of this microorganism is one of the remaining obstacles to industrial implementation.
Teun Kuil, Johannes Yayo, Johanna Pechan, Jan Küchler, Antonius J. A. van Maris   +4 more
doaj   +1 more source

A unique AA5 alcohol oxidase fused with a catalytically inactive CE3 domain from the bacterium Burkholderia pseudomallei

FEBS Letters, Volume 597, Issue 13, Page 1779-1791, July 2023., 2023
Copper radical oxidases (CROs) from Auxiliary Activities family 5 can oxidize various alcohol substrates. Bacterial CROs are less studied than eukaryotic enzymes. Here we have characterized an unusual bacterial CRO that acts only on primary alcohols and is fused to a carbohydrate esterase domain, which was completely inactive due to substitutions of ...
Scott Mazurkewich, Andrea Seveso, Johan Larsbrink   +2 more
wiley   +1 more source

Assembly of Cellulases from Separate Catalytic Domains and a Cellulose-Binding Module for Understanding Cooperative Crystalline Cellulose Degradation

Applied Sciences
The biochemical degradation of abundant cellulosic biomass for industrial use and energy production has been extensively researched in recent years. Some elaborate cellulose digestion approaches have been developed based on specialized bacteria, which ...
Katharina K. I. Schmitt, Hidehiko Hirakawa   +1 more
doaj   +1 more source

Evolution of glucuronoxylan side chain variability in vascular plants and the compensatory adaptations of cell wall–degrading hydrolases

New Phytologist, Volume 244, Issue 3, Page 1024-1040, November 2024.
Summary Polysaccharide structural complexity not only influences cell wall strength and extensibility but also hinders pathogenic and biotechnological attempts to saccharify the wall. In certain species and tissues, glucuronic acid side groups on xylan exhibit arabinopyranose or galactose decorations whose genetic and evolutionary basis is completely ...
Li Yu, Louis F. L. Wilson, Oliver M. Terrett, Joel Wurman‐Rodrich, Jan J. Łyczakowski, Xiaolan Yu, Kristian B. R. M. Krogh, Paul Dupree   +7 more
wiley   +1 more source

Altering the substitution and cross‐linking of glucuronoarabinoxylans affects cell wall architecture in Brachypodium distachyon

New Phytologist, Volume 242, Issue 2, Page 524-543, April 2024.
Summary The Poaceae family of plants provides cereal crops that are critical for human and animal nutrition, and also, they are an important source of biomass. Interacting plant cell wall components give rise to recalcitrance to digestion; thus, understanding the wall molecular architecture is important to improve biomass properties.
Theodora Tryfona, Yanina Pankratova, Deborah Petrik, Diego Rebaque Moran, Raymond Wightman, Xiaolan Yu, Alberto Echevarría‐Poza, Parveen Kumar Deralia, Francisco Vilaplana, Charles T. Anderson, Mei Hong, Paul Dupree   +11 more
wiley   +1 more source

Rational design paving the way for improving glucose tolerance and catalytic properties of a β-glucosidase from Acetivibrio thermocellus

Abstract Cellulases are an ensemble of enzymes that hydrolyse cellulose chains to fermentable glucose, hence, are widely used in bioethanol production. The last enzyme of the cellulose degradation pathway - β-glucosidase, is inhibited by its product – glucose.
Chinmay Kamale, Abhishek Rauniyar, Prasenjit Bhaumik   +2 more
openaire   +1 more source

Novel Microbial Enzymes with Industrial Applications. [PDF]

Microorganisms, 2023
González-Siso MI, Becerra M.
europepmc   +1 more source

Fermentative conversion of unpretreated plant biomass: A thermophilic threshold for indigenous microbial growth. [PDF]

Bioresour Technol, 2023
Bing RG, Carey MJ, Laemthong T, Willard DJ, Crosby JR, Sulis DB, Wang JP, Adams MWW, Kelly RM.   +8 more
europepmc   +1 more source

Unlocking cellulosic ethanol with Acetivibrio thermocellus: the role of thermodynamics, chaotropicity, and pyrophosphate

New Biotechnology
T Kuil, C Nurminen, A van Maris
openaire   +1 more source