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Restriction endonuclease activity in Clostridium thermocellum and Clostridium thermosaccharolyticum
Applied Microbiology and Biotechnology, 1996Clostridium thermocellum cell extracts exhibit specific endonuclease activity with very little non-specific exonuclease activity at 55 degrees C. The Dam methylation system of Escherichia coli offers complete protection from digestion by C. thermocellum ATCC 27405 cell extracts for all DNA tested (totaling > 100 kb, insuring that most potential ...
Lee R Lynd, Demain A L, Lynd L R
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Cellulolytic and physiological properties of Clostridium thermocellum
Archives of Microbiology, 1977Three strains of Clostridium thermocellum obtained from various sources were found to have nearly identical deoxyribonucleic acid guanosine plus cytosine contents that ranged from 38.1–39.5 mole-%. All strain examined fermented only cellulose and cellulose derivatives, but not glucose, or xylose or other sugars.
T K, Ng, T K, Weimer, J G, Zeikus
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Transformation of Clostridium Thermocellum by Electroporation
2012In this work, we provide detailed instructions for transformation of Clostridium thermocellum by electroporation. In addition, we describe two schemes for genetic modification: allelic replacement-where the gene of interest is replaced by an antibiotic marker and markerless gene deletion-where the gene of interest is removed and the selective markers ...
Daniel G, Olson, Lee R, Lynd
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Nucleotide sequence of the Clostridium thermocellum laminarinase gene
Biochemical and Biophysical Research Communications, 1991The sequence presented (1022 bp) shows the Clostridium thermocellum laminarinase gene (lam1) and its flanking regions. The gene lam1 comprises an open reading frame of 726 nt, encoding a 242-aa protein with predicted Mr 27661. The ORF startswith the translation initiation codon ATG.
V V, Zverlov +3 more
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The Cellulosome of Clostridium thermocellum
1988Publisher Summary The accelerated interest in microbial cellulases stems from their potential industrial application. This area is an excellent example in which basic and applied science are closely interlinked: the better understood the mechanism of cellulase action; the better can be the applicative value as a biotechnological process. This chapter
Raphael Lamed, Edward A. Bayer
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Thermostable Enzymes from Clostridium thermocellum
2021The production of bioenergy from wastes attracts worldwide attention to overcome energy crisis and increasing pollution (Thakur et al., Microbial fermentation and enzyme technology, Taylor and Francis Group, Boca Raton, FL, 257–268, 2020). Lignocellulosic biomass can serve as an alternative source for bioenergy production.
Abhijeet Thakur +3 more
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Cellulosomes from Clostridium thermocellum
1988Publisher Summary Biomass in the form of cellulose is the major constituent of plant matter, thereby comprising the earth's most abundant natural organic resource. Although the repeating unit of this biopolymer is a simple disaccharide (cellobiose), cellulose fibrils are organized into a complicated paracrystalline state.
Raphael Lamed, Edward A. Bayer
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