Results 311 to 320 of about 275,109 (338)
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Virology, 1973
A method is described for the purification of the defective protein of TMV mutants, from a class of mutants which engender an insoluble coat protein in their host. These proteins are unable to aggregate with TMV-RNA to form a functional virion. Amino acid compositions of one isolate each of mutant PM1 and PM2I and two of mutant PM4 showed a small ...
Milton Zaitlin +2 more
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A method is described for the purification of the defective protein of TMV mutants, from a class of mutants which engender an insoluble coat protein in their host. These proteins are unable to aggregate with TMV-RNA to form a functional virion. Amino acid compositions of one isolate each of mutant PM1 and PM2I and two of mutant PM4 showed a small ...
Milton Zaitlin +2 more
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A new polymeric coating for protein microarrays
Analytical Biochemistry, 2004Despite the increasing interest in arraying proteins in a high-density format, several technical issues still impede the development of protein microarray technology. One of the major problems is the availability of substrates that are able to bind native proteins with high density. In this study, we investigated the suitability of a novel surface as a
Cretich M +4 more
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Membrane deformation by protein coats
Current Opinion in Cell Biology, 2006Protein coats deform lipid membranes into spherical buds, which undergo fission at the neck to become vesicles. To induce membrane curvature, protein coats use basic tools including amphipathic helices and concave protein surfaces, and take advantage of the bulk properties of cellular membranes, such as loose lipid packing in the endoplasmic reticulum ...
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Edible Protein Films and Coatings [PDF]
, 2017 This chapter reviews research on protein film-formation fundamentals and film properties. It relates properties of protein-based films to molecular and environmental factors. The chapter compares barrier and mechanical properties of protein-based films to each other, polysaccharide-based films, and synthetic films. It summarizes applications of protein
J. L. Maubois, G. Ollivier
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Milk Proteins for Edible Films and Coatings
Critical Reviews in Food Science and Nutrition, 2004Due to the recent increase in ecological consciousness, research has turned toward finding edible materials. Viable edible films and coatings have been produced using milk proteins. These films and coatings may retard moisture loss, are good oxygen barriers, show good tensile strength and moderate elongation, are flexible, and generally have no flavor ...
Khwaldia, Khaoula +4 more
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The role of coat proteins in the biosynthesis of secretory proteins
Current Opinion in Cell Biology, 1995The biosynthesis of secretory proteins requires vesicle-mediated transport between the organelles of the secretory pathway. Biochemical and genetic analysis of the secretory pathway has identified two non-clathrin coats--COPI and COPII--that drive the formation of vesicles that mediate transport between the endoplasmic reticulum and the Golgi apparatus,
Nina R. Salama, Randy Schekman
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Recruitment of Coat Proteins to Liposomes and Peptidoliposomes
2014Intracellular transport within the cell is generally mediated by membrane vesicles. Their formation is typically initiated by activation of small GTPases that then recruit cytosolic proteins to the membrane surface to form a coat, interact with cargo and accessory proteins, and deform the lipid bilayer to produce a transport vesicle.
Huser, Sonja +3 more
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2016
Protein-coated microcrystals (PCMC) are a high-activity preparation of enzymes for use in low-water media. The protocols for the preparation of PCMCs of Subtilisin Carlsberg and Candida antarctica lipase B (CAL B) are described. The combi-PCMC concept is useful both for cascade and non-cascade reactions.
Munishwar N. Gupta, Joyeeta Mukherjee
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Protein-coated microcrystals (PCMC) are a high-activity preparation of enzymes for use in low-water media. The protocols for the preparation of PCMCs of Subtilisin Carlsberg and Candida antarctica lipase B (CAL B) are described. The combi-PCMC concept is useful both for cascade and non-cascade reactions.
Munishwar N. Gupta, Joyeeta Mukherjee
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Capillary Coatings for Protein Analysis
2001Protein analysis has become very important to the biotechnology, pharmaceutical, and food industries. Analytical techniques that provide fast, automated analysis, but still give high resolution separation of proteins derived from various sources, such as plasma, blood, and vaccine products, are in high demand.
Nebojsa Avdalovic +2 more
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Molecular structures of coat and coat-associated proteins: function follows form
Current Opinion in Cell Biology, 2006Endocytic clathrin-coated vesicles arise through the deformation of a small region of plasma membrane encapsulated by a cytosol-oriented clathrin lattice. The coat assembles from soluble protomers in a rapid and highly cooperative process, and invagination is tightly linked to the selective enrichment of cargo molecules within the nascent bud.
Linton M. Traub, Tom J. Brett
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