Results 11 to 20 of about 3,770 (215)
Intestinal organoid cocultures with microbes [PDF]
Nature Protocols, 2021 Adult-stem-cell-derived organoids model human epithelial tissues ex vivo, which enables the study of host-microbe interactions with great experimental control. This protocol comprises methods to coculture organoids with microbes, particularly focusing on human small intestinal and colon organoids exposed to individual bacterial species.
Jens Puschhof +10 more
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Engineering Cooperation in an Anaerobic Coculture [PDF]
Applied and Environmental Microbiology, 2021 Microbes seldom live alone in the environment, yet this scenario is approximated in the vast majority of pure-culture laboratory experiments. Here, we develop an anaerobic coculture system to begin understanding microbial physiology in a more complex setting but also to determine how anaerobic microbial communities can form.
Aunica L. Kane +2 more
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Antibiotics in microbial coculture [PDF]
The Journal of Antibiotics, 2016 Today, the frequency of discovery of new antibiotics in microbial culture is significantly decreasing. The evidence from whole-genome surveys suggests that many genes involved in the synthesis of unknown metabolites do exist but are not expressed under conventional cultivation conditions. Therefore, it is urgently necessary to study the conditions that
Kenji, Ueda, Teruhiko, Beppu
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Phagocytosis by stroma confounds coculture studies [PDF]
iScience, 2021 Signals provided by the microenvironment can modify and circumvent pathway activities that are therapeutically targeted by drugs. Bone marrow stromal cell coculture models are frequently used to study the influence of the bone marrow niche on ex vivo drug response.
Sophie A. Herbst +9 more
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mSphere, 2019 Candida albicans and Staphylococcus aureus are common causes of nosocomial infections with severe morbidity and mortality. Murine polymicrobial intra-abdominal infection (IAI) with C. albicans and S.
Olivia A. Todd +2 more
doaj +1 more source
Coculture of Bovine Demiembryos Prior to Freezing [PDF]
Acta Veterinaria Scandinavica, 1992 Pregnancy rates after transfer of frozen/thawed bisected embryos, demiembryos, have until now been very low. In the present study it was attempted to improve the freezability rate of demiembryos by culturing them on a monolayer of bovine oviduct epithelial cells (BOEC) prior to freezing.
M, Schmidt +4 more
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Microorganisms, 2022 Cocultures have been widely explored for their use in deciphering microbial interaction and its impact on the metabolisms of the interacting microorganisms.
Barbara Fifani +7 more
doaj +1 more source
Frontiers in Chemistry, 2018 Microbial culture conditions in the laboratory, which conventionally involve the cultivation of one strain in one culture vessel, are vastly different from natural microbial environments.
Daniel Shin +7 more
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Molecules, 2023 Bioreactor cocultures involving Penicillium rubens and Streptomyces rimosus were investigated with regard to secondary metabolite production, morphological development, dissolved oxygen levels, and carbon substrate utilization. The production profiles of
Tomasz Boruta +3 more
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Revista Colombiana de Ciencias Pecuarias, 2022 Background: Digestibility of fiber in the rumen is not due to enzymatic activity of individual bacteria, but rather to their interaction, which complements their enzymatic functioning.
Nicolás Torres-Salado +3 more
doaj +1 more source