Results 81 to 90 of about 5,297,063 (270)

Live Monitoring and Analysis of Fungal Growth, Viability, and Mycelial Morphology Using the IncuCyte NeuroTrack Processing Module

mBio, 2019
Efficient live-imaging methods are pivotal to understand fungal morphogenesis, especially as it relates to interactions with host immune cells and mechanisms of antifungal drugs.
Sebastian Wurster, Pappanaicken R. Kumaresan, Nathaniel D. Albert, Paul J. Hauser, Russell E. Lewis, Dimitrios P. Kontoyiannis   +5 more
doaj   +1 more source

Vascularized Liver‐on‐a‐Microsphere Reveals Alanine‐Glucose Metabolism‐Driven Regulation of Liver Function and Injury

Advanced Science, EarlyView.
A vascularized liver‐on‐a‐microsphere system is developed to recreate lobule‐like cellular organization and endothelial barrier function within compartmentalized hydrogel particles. The platform enhances hepatic performance and reveals alanine‐glucose cycle activation linked to improved detoxification capacity, offering a high‐throughput microscale ...
Jingyang Li, Zengnan Wu, Yingrui Zhang, Shulang Chen, Yongning Lin, Shiyu Chen, Tong Xu, Xianli Meng, Yi Zhang, Jin‐Ming Lin   +9 more
wiley   +1 more source

Comparison phenotypes: CIKs – adherent cells after coculture - MSCs.

, 2014
After 7/MSC coculture (day 14), adherent cells were harvested separately from suspended cells and analysed using flow cytometry. In addition, 14-day-old MSCs and CIKs were harvested and analysed for the same markers.
Christoph Schimmelpfennig (262154), Alexandra Stolzing (262150), Martin Bach (585999)   +2 more
core   +1 more source

PDGFRα+/Integrin α2+ Fibroblasts Orchestrate Tumor Budding in Oral Squamous Cell Carcinoma via Mechano‐Metabolic Symbiosis: E‐Cadherin/Integrin α2β1 Adhesion and Mitochondrial Transfer

Advanced Science, EarlyView.
PDGFA/PDGFRα signaling recruits CAFs to OSCC nests, and CAF‐OSCC contact drives tumor budding invasion. Mechanistically, heterotypic adhesion via E‐cadherin/integrin α2β1 activates YAP signaling in OSCC cells, inducing EMT. Concurrently, CAFs transfer mitochondria through TNTs, supplying ATP to fuel invasion.
Yufang Liu, Jiao Li, Juan Liu, Qi Dong, Haoyang Zhang, Yanjin Wang, Huibing Li, Ye Guan, Lei Cao, Manqing Zhang, Fangning Guo, Xue Liu, Zhen Yang, Mengmeng Lu, Hui Liu, Laiping Zhong, Tong Ji, Tingjiao Liu   +17 more
wiley   +1 more source

Optimization of the Static Human Osteoblast/Osteoclast Co-culture System

Iranian Journal of Medical Sciences, 2018
Osteoblasts (OBs) and osteoclasts (OCs) are 2 major groups of bone cells. Their cell-to-cell interactions are important to ensure the continuity of the bone-remodeling process.
James Jam Jolly, Kok-Yong Chin, Mohd Fozi Nur Farhana, Ekram Alias, Kien Hui Chua, Wan Nuraini Wan Hasan, Soelaiman Ima-Nirwana   +6 more
doaj  

Advancements in vaginal microbiota, Trichomonas vaginalis, and vaginal cell interactions: Insights from co-culture assays

Microbial Cell
Vaginal microbiota involves seven communities-state types (CST), four dominated by Lactobacillus. L. crispatus, particularly, offers enhanced protection against infections.
Fernanda Gomes Cardoso, Tiana Tasca
doaj   +1 more source

Iron Flocs and the Three Domains: Microbial Interactions in Freshwater Iron Mats

mBio, 2020
Freshwater iron mats are dynamic geochemical environments with broad ecological diversity, primarily formed by the iron-oxidizing bacteria. The community features functional groups involved in biogeochemical cycles for iron, sulfur, carbon, and nitrogen.
Chequita N. Brooks, Erin K. Field
doaj   +1 more source

Schematic description of cell monoculture and coculture systems.

, 2016
HGEC cells were seeded on the lower side of a Millicell support (0.4 μm membrane pore size), and HK-2 cells into the upper side. Cells were either cultured in monoculture (A and B) or in coculture (C).
Flavia Sacerdoti (321463), Cristina Ibarra (358216), James C. Paton (198315), María M. Amaral (439445), Romina S. Álvarez (2851214), Carolina Jancic (337983), Adrienne W. Paton (439447)   +6 more
core   +1 more source

RPLP2 Mediates the Beneficial Effects of Exercise on Stress Resistance Through Muscle–Brain Communication

Advanced Science, EarlyView.
A novel exercise‐inducible myokine acidic ribosomal protein P2 (RPLP2), initially identified from human trials, is presented here, whose circulating levels negatively correlate with clinical anxiety severity. Muscle‐derived RPLP2 enhances hippocampal ribosomal assembly and adult neurogenesis to rescue stress‐induced anxiety deficits.
Peiyu Luo, Wei Wu, Huan Peng, Dan He, Yuxi Guo, Boyue Zhao, Xiaodan Wang, Li Ma, Yuhang Qin, Yifang Zhai, Lixia Zhuo, Ying Zhang, Yijie Guo, Linlin Jing, Fangyao Chen, Erfei Zhang, Wei Wang, Xiancang Ma, Yan Li   +18 more
wiley   +1 more source

Fabrication of a novel 3D-printed perfusion bioreactor for complex cell culture models

Scientific Reports
We introduce a novel fabrication method for developing a 3D-printed perfusion bioreactor (3D-PBR) to facilitate the in situ growth and differentiation of human bone marrow (BM)-derived mesenchymal stem cells (MSCs) while enabling coculture with vascular ...
Brian H. Jun, Jacob E. Torrez, David J. Ross, Brian M. Patterson, Mohammad O. Ishak, Arasely M. Rodriguez, Jennifer F. Harris, Katie L. Davis-Anderson   +7 more
doaj   +1 more source