Results 151 to 160 of about 69,317 (259)

Enhancing CRISPR/Cas‐Mediated Gene Knockout With Short Non‐Homologous Oligonucleotides

Plant Biotechnology Journal, EarlyView.
ABSTRACT Chlamydomonas reinhardtii is a model green microalga that has great industrial potential as a sustainable bio‐factory for recombinant protein and high‐value chemical production. Efficient genome editing tools are required to redesign this organism for synthetic biology applications. CRISPR‐Cas editing technologies have already been adapted for
Yen Peng Chew, Aron Ferenczi, Marie Dannay, Cristina Ponce‐Lilly, Adam Kovac, Dávid Tóth, Szilvia Z. Tóth, Attila Molnar   +7 more
wiley   +1 more source

Engineering a Gibberellin‐Switchable Dual‐Use Line Based on Ent‐Kaurene Oxidase Gene ZmKO1 Enables Two‐Line Hybrid Seed Production in Maize

Plant Biotechnology Journal, EarlyView.
ABSTRACT Male‐sterile systems are pivotal for maize hybrid seed production, yet existing systems are constrained by significant limitations. Here, we identified a novel single‐gene‐controlled recessive genic male sterility (GMS) mutant, defective in filament elongation 1 (def1), which exhibits non‐elongated filaments, unopened glumes, and non‐exserted ...
Chuan Li, Siyu Wang, Yong Xiang, Tao Yu, Rujun Wei, Wenqian Mei, Xiaowei Liu, Peng Yuan, Hongyang Yi, Shufeng Zhou, Chao Xia, Moju Cao, Hai Lan   +12 more
wiley   +1 more source

Spatial Regulation of Silicon Accumulation in Peduncle Confers Sheathed Spike in Barley

Plant Biotechnology Journal, EarlyView.
ABSTRACT Peduncle, the uppermost internode in cereals, connects the stem to the inflorescence and is critical for the transport of water, nutrients and photosynthetic assimilates. While peduncle length associates with plant height and its elongation is primarily regulated by phytohormones, we report a previously unrecognized mechanism involving the ...
Wenxue Wu, Congcong Jiang, Guangqi Gao, Namiki Mitani‐Ueno, Yu Guo, Jinhong Kan, Yehui Xiong, Martin Mascher, Zhongfu Ni, Jian Feng Ma, Ke Wang, Ping Yang   +11 more
wiley   +1 more source

Anatomical and functional mapping of vagal nociceptive sensory nerve subsets innervating the mouse lower airways by intersectional genetics

The Journal of Physiology, EarlyView.
Abstract figure legend We used an intersectional approach with recombinase‐expressing mice and adeno‐associated virus to map and modulate distinct nociceptive afferents in the vagal ganglia. TRPV1+P2X2+ neurons resided in the nodose ganglion (N), innervated the lungs (many projected into the alveoli) but not the trachea, and projected to the nucleus ...
Mayur J. Patil, Justin Shane Hooper, Seol‐Hee Kim, Parmvir K. Bahia, Nika Pavelkova, Sanjay S. Nair, Teresa S. Darcey, Karina V. Lurye, Meghana Madaram, Jailene Fiallo, Stephen H. Hadley, Thomas E. Taylor‐Clark   +11 more
wiley   +1 more source

D-stem mutation in an essential tRNA increases translation speed at the cost of fidelity.

PLoS Genetics
The efficiency with which aminoacyl-tRNA and GTP-bound translation elongation factor EF-Tu recognizes the A-site codon of the ribosome is dependent on codons and tRNA species present in the polypeptide (P) and exit (E) codon sites.
Madison N Schrock, Krishna Parsawar, Kelly T Hughes, Fabienne F V Chevance   +3 more
doaj   +1 more source

Coordinated regulation of PIEZO2 by alternative splicing, post‐translational modification, membrane trafficking and protein partners

The Journal of Physiology, EarlyView.
Abstract figure legend Regulatory mechanisms such as alternative splicing, post‐translational modification, membrane trafficking, and protein interactions control channel gating, membrane abundance, and overall activity of PIEZO2. Proper regulation supports PIEZO2‐dependent proprioceptive, somatosensory, nociceptive, pruriceptive and interoceptive ...
Eunice I. Oribamise, Qinglu Li, Mary G. Garry   +2 more
wiley   +1 more source

From Multiple Congenital Anomalies to Pituitary Gland Malformation: Wide Spectrum of Clinical Features in a Family With FOXA2 Variant

American Journal of Medical Genetics Part A, Volume 200, Issue 6, Page 1452-1457, June 2026.
ABSTRACT FOXA2 (hepatocyte nuclear factor‐3β, HNF‐3β) encodes a transcriptional activator involved in early embryogenesis, particularly in the patterning and differentiation of midline structures such as the neural tube, foregut, and pituitary gland. Its role in human pathogenesis was first suspected when patients with deletion of chromosome 20p11.2 ...
Christopher Connolly, Hemant A. Parmar, Lauren Hipp, Tomoyasu Higashimoto   +3 more
wiley   +1 more source

CYLD Cutaneous Syndrome: Report of a New Splicing Pathogenic Variant and Additional Evidence Supporting the Absence of Genotype–Phenotype Correlation

International Journal of Dermatology, EarlyView.
Agathe Hercent, Adrien Borgel, Jerôme Lamoril, Florent Grange, Frédérique Carré‐Pigeon, Caroline Kannengiesser, Dimitri Tchernitchko   +6 more
wiley   +1 more source

Novel MYL1 Intron Variant With Expanded Phenotype

American Journal of Medical Genetics Part A, Volume 200, Issue 6, Page 1378-1383, June 2026.
ABSTRACT Congenital myopathy‐14 (CMYO14) is an ultrarare autosomal recessive disorder caused by biallelic variants in MYL1, with only four patients reported to date. We describe what is likely the fifth reported patient, a neonate with severe hypotonia, respiratory insufficiency, and skeletal anomalies showing distinct histological changes of skeletal ...
Maria Barington, Marie Balslev‐Harder, Thomas Krag, Thomas van Overeem Hansen, Camilla Bernt Wulff, Ulrik Lausten‐Thomsen, Tina Duelund Hjortshøj, Elsebet Østergaard   +7 more
wiley   +1 more source

Cytosolic Phosphoenoylpyruvate Carboxykinase Deficiency: Clinical, Biochemical, and Genetic Features of Five Non‐Finnish Patients

American Journal of Medical Genetics Part A, Volume 200, Issue 6, Page 1192-1203, June 2026.
ABSTRACT Cytosolic phosphoenoylpyruvate carboxykinase (PEPCK‐C) is an essential, rate‐limiting enzyme in the gluconeogenesis pathway. PEPCK‐C deficiency presents with hypoglycaemia, hyperlactataemia and hepatopathy, and was first reported in association with bi‐allelic PCK1 variants in 2014.
Isaac Bernhardt, Polona Le Quesne Stabej, Claire Hart, Mark De Hora, Sarah Hulley, Mark Anderson, Harry G. Leitch, Hugh Lemonde, Bryony Ryder, James Davison   +9 more
wiley   +1 more source