Results 51 to 60 of about 91,233 (268)

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

pH‐mediated activation of the lysosomal arginine sensor SLC38A9

FEBS Letters, EarlyView.
Cells monitor nutrient levels via the lysosomal transporter SLC38A9 to activate the mechanistic target of rapamycin complex 1 (mTORC1). This study reveals that SLC38A9 function is regulated by pH. We identified histidine 544 as a critical pH sensor that undergoes conformational changes to control amino acid efflux from lysosomes; therefore, it ...
Xuelang Mu, Ampon Sae Her, Tamir Gonen
wiley   +1 more source

Cyber–physical microgrid components fault prognosis using electromagnetic sensors

IET Cyber-Physical Systems, 2018
Higher operational requirements in cyber–physical microgrid system stress the electrical system and may push it to the edge of stability. Therefore, prognosis of the imminent failures is vital.
Tanushree Agarwal, Payam Niknejad, Abolfazl Rahimnejad, Abolfazl Rahimnejad, M.R. Barzegaran, Luigi Vanfretti   +5 more
doaj   +1 more source

On-Surface Assembly of Coiled-Coil Heterodimers [PDF]

Langmuir, 2012
The coiled coil is a widespread protein motif responsible for directing the assembly of a wide range of protein complexes. To date, research has focused largely on the solution phase assembly of coiled-coil complexes. Here, we describe an investigation into coiled-coil heterodimer assembly where one of the peptides is immobilized directly onto a gold ...
Simon J, White, D William A, Morton, Boon Chong, Cheah, Agnieszka, Bronowska, A Giles, Davies, Peter G, Stockley, Christoph, Wälti, Steven, Johnson   +7 more
openaire   +2 more sources

Residual tail twisting in ascidian larvae is stabilized by asymmetric myofibrils that resist bilateral symmetry restoration

FEBS Letters, EarlyView.
Ascidian Ciona larvae initially show strong clockwise tail twisting, which is largely corrected during development. However, a small residual twist remains. This study shows that organized helical myofibrils in tail muscles mechanically stabilize this residual asymmetry, preventing complete restoration of bilateral symmetry and revealing how embryos ...
Yuki S. Kogure, Misaki K. Nishio, Satoru Okuda, Kohji Hotta   +3 more
wiley   +1 more source

Proposed methodology for online frequency response analysis based on magnetic coupling to detect winding deformations in transformers

High Voltage, 2019
Frequency response analysis is widely used as a method for the offline diagnosis of winding deformations in power transformers. To apply it to a working transformer, people need to determine how to inject the excitation signal and measure the response ...
Yangchun Cheng, Jiangang Bi, Jiangang Bi, Wenzhi Chang, Yuan Xu, Xiaohua Pan, Xianwei Ma, Shuai Chang   +7 more
doaj   +1 more source

Predicting oligomerization states of coiled coils [PDF]

Protein Science, 1995
AbstractAn algorithm based on the profile method was developed that faithfully distinguishes between the amino acid sequences of dimeric and trimeric coiled coils. Normalized sequence profiles derived from nonhomologous, two‐and three‐stranded, coiled‐coil sequences with unambiguous registers were used to assign dimer and trimer propensities to test ...
D N, Woolfson, T, Alber
openaire   +2 more sources

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

Multilayer air gap winding designs for electric machines: theory, design, and characterisation

The Journal of Engineering, 2019
Here, the authors present a magnetic circuit design with a multilayer air gap winding. This design provides a new degree of freedom in the design process.
Norman Borchardt, Roland Kasper, Jörg Sauerhering, Wolfgang Heinemann, Kimberly L. Foster   +4 more
doaj   +1 more source

The ubiquitin‐proteasome system and autophagy as guardians of the cellular proteome

FEBS Letters, EarlyView.
This Perspective covers the three principles governing the crosstalk between the ubiquitin‐proteasome system and autophagy in cellular proteostasis: (1) a shared ubiquitin code routing substrates via shuttle factors or autophagy receptors; (2) spatial compartmentalization into phase‐separated degradation hubs and organelle‐specific modules (exemplified
Ivan Dikic
wiley   +1 more source