Results 61 to 70 of about 338,876 (258)

Pectinase secreted by psychrotolerant fungi: identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from Tetracladium sp.

Microbial Cell Factories, 2019
Background Pectinolytic enzymes, which are used in several industries, especially in the clarification process during wine and fruit juice production, represent approximately 10% of the global enzyme market.
Mario Carrasco, Juan Manuel Rozas, Jennifer Alcaíno, Víctor Cifuentes, Marcelo Baeza   +4 more
doaj   +1 more source

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

Identification of 2-Aminothiazole-4-Carboxylate Derivatives Active against Mycobacterium tuberculosis H37Rv and the beta-Ketoacyl-ACP Synthase mtFabH [PDF]

, 2009
Background: Tuberculosis (TB) is a disease which kills two million people every year and infects approximately over one-third of the world's population.
Johnston, Blair F., Coxon, Geoffrey D., Geoffrey D Coxon, Besra, Gurdyal S., Nahoum G Anthony, Gillespie, Stephen, Amani Alnimr, Al-Balas Qosay, Todd, MH, Coxon, GD, Gillespie Stephen H., McHugh, Timothy D., Bilal Al-Jaidi, Besra, GS, Johnston, BF, Mackay, Simon, Alnimr, Amani, Taylor, Rebecca C., Mackay Simon P., Blair F Johnston, Qosay Al-Balas, Alnimr, A, Coxon, Geoffrey, Brown, Alistair K., Simon P Mackay, Besra Gurdyal S., Mackay, SP, Taylor, RC, Gillespie, Stephen H., Johnston, Blair, Brown, Alistair, Al-Jaidi, Bilal, Al-Jaidi Bilal, McHugh, TD, Brown, AK, Anthony, Nahoum, Besra, Gurdyal; id_orcid, Besra, Gurdyal, Timothy D McHugh, McHugh, Timothy, Taylor, Rebecca, Rebecca C Taylor, Coxon Geoffrey D., Stephen H Gillespie, Alistair K Brown, Al-Balas, Q, Al-Balas, Qosay, Grainne Abbott, Abbott, Grainne, Gurdyal S Besra, Anthony, NG, Alnimr Amani, Anthony, Nahoum G., Johnston Blair F., Anthony Nahoum G., Mackay, Simon P., Taylor Rebecca C., McHugh Timothy D., Gillespie, SH, Brown Alistair K., Abbott, G, Abbott Grainne, Al-Jaidi, B   +62 more
core   +1 more source

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

Molecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu, Nicolas J. Niklaus, Anna M. Schläfli, Igor Tokarchuk, Magali Humbert, Federico La Manna, Bich Vu, David Maul, Ramin Radpour, Marianna Kruithof‐de Julio, Inti Zlobec, Jörn Dengjel, Bruce E. Torbett, Mario P. Tschan   +13 more
wiley   +1 more source

Three hydrolases and a transferase: comparative analysis of active-site dynamics via the BioSimGrid database

, 2007
Comparative molecular dynamics (MD) simulations enable us to explore the conformational dynamics of the active sites of distantly related enzymes.
Baaden, Marc, Muan Hong Ngd, Essex, Jonathan, Sansom, MS, Sansom, Mark, Murdock, S, Cox, K, Cox, Katherine, Tai, K, Ng, Muan Hong, Tai, Kaihsu, Jonathan W. Essexc, Steven Johnstonc, Bing Wua, Marc Baadenb, Essex, Jonathan W, Baaden, M, Essex, JW, Essex, Jonathan W., Sansom, Mark S.P., Murdock, Stuart, Wu, Bing, Stuart Murdockc, Johnston, Steven, Fangohr, Hans, Wu, B, Kaihsu Taia, Richard Boardmanc, Ng, MH, Boardman, R, Fangohr, H, Mark S. P. Sansoma, Katherine Coxa, Sansom, Mark S. P., Johnston, S, Hans Fangohrd, Boardman, Richard   +36 more
core   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

Structural Studies of Catalysis and Processing in Galactose [PDF]

, 2007
The fungal copper containing enzyme galactose oxidase is one of the earliest examples of a protein derived radical cofactor. A covalent bond, formed between the active site cysteine and the Cc of a tyrosine act as a site for radical formation, allowing ...
Akumanyi, Nana
core  

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Enzyme activities and biotechnological applications of cold-active microfungi

, 2012
Fungi are eukaryotic organisms and considered to be less adaptable to extreme environments when compared to bacteria. While there are no thermophilic microfungi in a strict sense, some fungi have adapted to life in the cold.
Mohammed, Suja, Te’o, Junior, Nevalainen, Helena, Bradner, Ron, Wadud, Sania, McRae, Christopher   +5 more
core