Results 61 to 70 of about 210,476 (300)

Cold-adapted protease enables quantitation of surface proteins in the absence of membrane trafficking

BioTechniques, 2011
We report here an improved method for analyzing protein surface expression utilizing a cold-adapted trypsin. Preservation of activity of the enzyme at 0–4°C permits modification of the protease method of surface analysis to temperatures at which ...
Faraz Ahmad, Sarah Kate Coleman, Kai Kaila, Peter Blaesse   +3 more
doaj   +1 more source

Nanocellulose as building block for novel materials [PDF]

, 2012
This thesis describes the fabrication of novel green materials using nanocellulose as the building block. Bacterial cellulose (BC) was used as the nanocellulose predominantly in this work. BC is highly crystalline pure cellulose with an inherent fibre
Lee, Koon-Yang, Lee, Koon-Yang
core   +1 more source

Genetic attenuation of ALDH1A1 increases metastatic potential and aggressiveness in colorectal cancer

Molecular Oncology, EarlyView.
Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cancer stem cell marker in several malignancies. We established a novel epithelial cell line from rectal adenocarcinoma with unique overexpression of this enzyme. Genetic attenuation of ALDH1A1 led to increased invasive capacity and metastatic potential, the inhibition of proliferation activity, and ultimately ...
Martina Poturnajova, Zuzana Kozovska, Ondrej Pos, Kristina Pavlov, Sachin Gulati, Peter Makovicky, Kristina Jakic, Monika Burikova, Eva Sedlackova, Barbora Svitkova, Silvia Tyciakova, Vojtech Bystry, Nicolas Blavet, Boris Tichy, Matej Hrnciar, Jaroslav Budis, Miroslav Tomas, Peter Dubovan, Georgina Kolnikova, Veronika Repaska, Nikoleta Mojzesova, Eva Zomborska, Daniel Pindak, Michal Mego, Tomas Szemes, Miroslava Matuskova   +25 more
wiley   +1 more source

Cold‐active microbial enzymes and their biotechnological applications

Microbial Biotechnology
Microorganisms known as psychrophiles/psychrotrophs, which survive in cold climates, constitute majority of the biosphere on Earth. Their capability to produce cold‐active enzymes along with other distinguishing characteristics allows them to survive in ...
Mohammed Kuddus, Roohi, Naushin Bano, Gouse Basha Sheik, Babu Joseph, Burhan Hamid, Raveendran Sindhu, Aravind Madhavan   +7 more
doaj   +1 more source

Cloning, overexpression, purification, and characterization of a polyextremophilic β-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi

BMC Biotechnology, 2013
Background Halorubrum lacusprofundi is a cold-adapted halophilic archaeon isolated from Deep Lake, a perennially cold and hypersaline lake in Antarctica.
Karan Ram, Capes Melinda D, DasSarma Priya, DasSarma Shiladitya   +3 more
doaj   +1 more source

Transcriptome pathways unique to dehydration tolerant relatives of modern wheat [PDF]

, 2009
Among abiotic stressors, drought is a major factor responsible for dramatic yield loss in agriculture. In order to reveal differences in global expression profiles of drought tolerant and sensitive wild emmer wheat genotypes, a previously deployed shock ...
AN Olsen, B Barnabas, B Vinocur, C Guy, D Bartels, DB Lobell, E Ormeno, EA Bray, G Toledo-Ortiz, GP Xue, GR Cramer, Hans J. Bohnert, HG Jones, Hikmet Budak, HJ Bohnert, IK Vasil, J Dubcovsky, J Zhou, JL Araus, Jyothi Thimmapuram, K Deuschle, K Higuchi, K Higuchi, K Poustini, K Shinozaki, L Cseke, L Rodriguez-Uribe, L Xiong, M Mohammadi, M Seki, MB Eisen, N Kaur, NC Collins, Neslihan Z. Ergen, O Cebeci, P Langridge, P Rampino, PBK Kishor, PY Muller, R Kaldenhoff, R Munns, S Chao, S Mahajan, S Ramanjulu, T Eulgem, The Gene Ontology Consortium, V Talame, W Li, W Wang, YG Shen, YH Lee, ZN Ergen, ZN Ergen, ZN Ozturk   +53 more
core   +1 more source

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

Molecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano, Giuseppina Minopoli, Simona Romano, Laura Marrone, Katia Aquilano, Maria Lina Tornesello, Raffaella Faraonio   +6 more
wiley   +1 more source

Development of a Cold-Adapted Pseudoalteromonas Expression System for the Pseudoalteromonas Proteins Intractable for the Escherichia coli System.

PLoS ONE, 2015
Although the Escherichia coli expression system is the most commonly used expression system, some proteins are still difficult to be expressed by this system, such as proteins with high thermolability and enzymes that cannot mature by autoprocessing ...
Zi-Chao Yu, Bai-Lu Tang, Dian-Li Zhao, Xiuhua Pang, Qi-Long Qin, Bai-Cheng Zhou, Xi-Ying Zhang, Xiu-Lan Chen, Yu-Zhong Zhang   +8 more
doaj   +1 more source

RNAseq reveals hydrophobins that are involved in the adaptation of aspergillus nidulans to lignocellulose [PDF]

, 2016
Background Sugarcane is one of the world’s most profitable crops. Waste steam-exploded sugarcane bagasse (SEB) is a cheap, abundant, and renewable lignocellulosic feedstock for the next-generation biofuels.
Brown, Neil Andrew, Corrêa Dos Santos, Renato Augusto, Goldman, Gustavo H, Rajendran, Ranjith, Ramage, Gordon, Reis, Thaila F, Riaño-Pachón, Diego Mauricio, Ries, Laure N A   +7 more
core   +4 more sources

RIPK4 function interferes with melanoma cell adhesion and metastasis

Molecular Oncology, EarlyView.
RIPK4 promotes melanoma growth and spread. RIPK4 levels increase as skin lesions progress to melanoma. CRISPR/Cas9‐mediated deletion of RIPK4 causes melanoma cells to form less compact spheroids, reduces their migratory and invasive abilities and limits tumour growth and dissemination in mouse models.
Norbert Wronski, Sławomir Lasota, Ewelina Madej, Anna A. Brożyna, Małgorzata Szczygieł, Agnieszka Harazin‐Lechowska, Jan Czerbniak, Janusz Rys, Jaroslaw Czyz, Agnieszka Wolnicka‐Glubisz   +9 more
wiley   +1 more source