Results 51 to 60 of about 62,945 (257)

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

Molecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano, Giuseppina Minopoli, Simona Romano, Laura Marrone, Katia Aquilano, Maria Lina Tornesello, Raffaella Faraonio   +6 more
wiley   +1 more source

Cold‐active microbial enzymes and their biotechnological applications

Microbial Biotechnology
Microorganisms known as psychrophiles/psychrotrophs, which survive in cold climates, constitute majority of the biosphere on Earth. Their capability to produce cold‐active enzymes along with other distinguishing characteristics allows them to survive in ...
Mohammed Kuddus, Roohi, Naushin Bano, Gouse Basha Sheik, Babu Joseph, Burhan Hamid, Raveendran Sindhu, Aravind Madhavan   +7 more
doaj   +1 more source

Cold-adapted protease enables quantitation of surface proteins in the absence of membrane trafficking

BioTechniques, 2011
We report here an improved method for analyzing protein surface expression utilizing a cold-adapted trypsin. Preservation of activity of the enzyme at 0–4°C permits modification of the protease method of surface analysis to temperatures at which ...
Faraz Ahmad, Sarah Kate Coleman, Kai Kaila, Peter Blaesse   +3 more
doaj   +1 more source

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

Cloning, overexpression, purification, and characterization of a polyextremophilic β-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi

BMC Biotechnology, 2013
Background Halorubrum lacusprofundi is a cold-adapted halophilic archaeon isolated from Deep Lake, a perennially cold and hypersaline lake in Antarctica.
Karan Ram, Capes Melinda D, DasSarma Priya, DasSarma Shiladitya   +3 more
doaj   +1 more source

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

Molecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu, Nicolas J. Niklaus, Anna M. Schläfli, Igor Tokarchuk, Magali Humbert, Federico La Manna, Bich Vu, David Maul, Ramin Radpour, Marianna Kruithof‐de Julio, Inti Zlobec, Jörn Dengjel, Bruce E. Torbett, Mario P. Tschan   +13 more
wiley   +1 more source

Development of a Cold-Adapted Pseudoalteromonas Expression System for the Pseudoalteromonas Proteins Intractable for the Escherichia coli System.

PLoS ONE, 2015
Although the Escherichia coli expression system is the most commonly used expression system, some proteins are still difficult to be expressed by this system, such as proteins with high thermolability and enzymes that cannot mature by autoprocessing ...
Zi-Chao Yu, Bai-Lu Tang, Dian-Li Zhao, Xiuhua Pang, Qi-Long Qin, Bai-Cheng Zhou, Xi-Ying Zhang, Xiu-Lan Chen, Yu-Zhong Zhang   +8 more
doaj   +1 more source

Single‐cell DNA methylation profiling: Technologies, computation, and applications in precision oncology

Molecular Oncology, EarlyView.
Single‐cell DNA methylation (scDNAme) profiling maps epimutational clonal evolution, revealing mechanisms of malignancy and therapeutic resistance across diverse cancer types. By providing a high‐resolution landscape of intratumoral heterogeneity, these technologies empower precise patient stratification, guide the development of enhanced ...
Ik Soo Kim
wiley   +1 more source

High‐Spin States of Manganese(III) Enable Robust Cold‐Adapted Activity of MnO2 Nanozymes

Advanced Science
Developing novel cold‐adapted nanozymes and elucidating their mechanisms of action remains a great challenge. Inspired by natural oxidases that utilize high‐spin and high‐valent metal‐oxygen intermediates to achieve high efficiency at low temperatures ...
Qing Tian, Shuaiqi Huangfu, Ge Kang, Haoyu Wang, Huile Liu, Xuejing Wang, Aipeng Li, Yao Chen, Kelong Fan, Lianbing Zhang   +9 more
doaj   +1 more source

Identification and molecular characterization of a psychrophilic GH1 β-glucosidase from the subtropical soil microorganism Exiguobacterium sp. GXG2

AMB Express, 2019
The products of bacterial β-glucosidases with favorable cold-adapted properties have industrial applications. A psychrophilic β-glucosidase gene named bglG from subtropical soil microorganism Exiguobacterium sp.
Bangqiao Yin, Hengsen Gu, Xueyan Mo, Yue Xu, Bing Yan, Quanwen Li, Qian Ou, Bo Wu, Chen Guo, Chengjian Jiang   +9 more
doaj   +1 more source