Results 31 to 40 of about 62,945 (257)

Characterization of a Salt-Tolerant and Cold-Adapted Xylanase from Bacillus cellulosilyticus

BioResources, 2016
A xylanase (Xyn10A) gene from the saline-alkali-tolerant microorganism Bacillus cellulosilyticus DSM 2522 was cloned and expressed in Escherichia coli BL21 (DE3).
Jianzhong Sun, Sailing Shi, Jian Wu, Rongrong Xie, Alei Geng, Dao Chen Zhu   +5 more
doaj   +1 more source

Highly Stable, Cold-Active Aldehyde Dehydrogenase from the Marine Antarctic Flavobacterium sp. PL002

Fermentation, 2021
Stable aldehyde dehydrogenases (ALDH) from extremophilic microorganisms constitute efficient catalysts in biotechnologies. In search of active ALDHs at low temperatures and of these enzymes from cold-adapted microorganisms, we cloned and characterized a ...
Georgiana Necula-Petrareanu, Paris Lavin, Victoria Ioana Paun, Giulia Roxana Gheorghita, Alina Vasilescu, Cristina Purcarea   +5 more
doaj   +1 more source

A Novel Cold-Adapted and Salt-Tolerant RNase R from Antarctic Sea-Ice Bacterium Psychrobacter sp. ANT206

Molecules, 2019
A novel RNase R, psrnr, was cloned from the Antarctic bacterium Psychrobacter sp. ANT206 and expressed in Escherichia coli (E. coli). A bioinformatics analysis of the psrnr gene revealed that it contained an open reading frame of 2313 bp and encoded a ...
Yatong Wang, Yanhua Hou, Ping Nie, Yifan Wang, Xiulian Ren, Qifeng Wei, Quanfu Wang   +6 more
doaj   +1 more source

Characterization of SdGA, a cold-adapted glucoamylase from Saccharophagus degradans

Biotechnology Reports, 2021
We investigated the structural and functional properties of SdGA, a glucoamylase (GA) from Saccharophagus degradans, a marine bacterium which degrades different complex polysaccharides at high rate.
Natael M. Wayllace, Nicolas Hedín, María V. Busi, Diego F. Gomez-Casati   +3 more
doaj   +1 more source

Purification and characterization of a novel cold adapted fungal glucoamylase

Microbial Cell Factories, 2017
Background Amylases are used in various industrial processes and a key requirement for the efficiency of these processes is the use of enzymes with high catalytic activity at ambient temperature.
Mario Carrasco, Jennifer Alcaíno, Víctor Cifuentes, Marcelo Baeza   +3 more
doaj   +1 more source

Molecular cloning, expression and biochemical characterisation of a cold-adapted novel recombinant chitinase from Glaciozyma antarctica PI12

Microbial Cell Factories, 2011
Background Cold-adapted enzymes are proteins produced by psychrophilic organisms that display a high catalytic efficiency at extremely low temperatures. Chitin consists of the insoluble homopolysaccharide β-(1, 4)-linked N-acetylglucosamine, which is the
Ramli Aizi, Mahadi Nor, Rabu Amir, Murad Abdul, Bakar Farah, Illias Rosli   +5 more
doaj   +1 more source

Fundamentals of Cold-Adapted Enzymes

, 2008
The authors wish to thank N. Gerardin, A. Dernier and R. Marchand for their skilful technical assistance and the French Institute for Polar Research (IPEV – Institut Polaire Francais) for generously accommodating our research fellows at the French Antarctic station J.S. Dumont d’Urville in Terre Adelie.
Collins, T., Roulling, Frédéric, Piette, Florence, Marx, J. C., Feller, Georges, Gerday, Charles, D'Amico, Salvino   +6 more
openaire   +2 more sources

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source