Psychrophilic enzymes: molecular basis of cold adaptation [PDF]
Cellular and Molecular Life Sciences CMLS, 1997 Psychrophilic organisms have successfully colonized polar and alpine regions and are able to grow efficiently at sub-zero temperatures. At the enzymatic level, such organisms have to cope with the reduction of chemical reaction rates induced by low temperatures in order to maintain adequate metabolic fluxes. Thermal compensation in cold-adapted enzymes
Feller, Georges, Gerday, Charles
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Cloning, expression and characterization of a cold-adapted endo-1, 4-β-glucanase from Citrobacter farmeri A1, a symbiotic bacterium of Reticulitermes labralis [PDF]
PeerJ, 2016 Background Many biotechnological and industrial applications can benefit from cold-adapted EglCs through increased efficiency of catalytic processes at low temperature.
Xi Bai +5 more
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Marine Drugs, 2020 Alginate oligosaccharides produced by enzymatic degradation show versatile physiological functions and biological activities. In this study, a new alginate lyase encoding gene alyS02 from Flavobacterium sp. S02 was recombinantly expressed at a high level
Hai-Xiang Zhou +4 more
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Understanding High-Salt and Cold Adaptation of a Polyextremophilic Enzyme [PDF]
Microorganisms, 2020 The haloarchaeon Halorubrum lacusprofundi is among the few polyextremophilic organisms capable of surviving in one of the most extreme aquatic environments on Earth, the Deep Lake of Antarctica (−18 °C to +11.5 °C and 21–28%, w/v salt content). Hence, H.
Ram Karan +9 more
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Frontiers in Microbiology, 2022 Endo-β-1,4-glucanase is a crucial glycoside hydrolase (GH) involved in the decomposition of cellulosic materials. In this study, to discover a novel cold-adapted β-1,4-D-glucan-degrading enzyme, the gene coding for an extracellular endo-β-1,4-glucanase ...
Do Young Kim +8 more
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Rational engineering of a cold-adapted α-amylase from the Antarctic ciliate Euplotes focardii for simultaneous improvement of thermostability and catalytic activity [PDF]
, 2017 The α-amylases are endo-acting enzyme which hydrolyze starch by randomly cleaving the 1,4-α-D-glucosidic linkages between the adjacent glucose units in linear amylose chain.
Ballarini, Patrizia +5 more
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Czech Journal of Food Sciences, 2008 Enzymes with high specific activities at low temperatures have potential uses in the food industry. Cold-adapted microorganisms are potentially useful sources of cold-active enzyme.
Wen-Yu Liu +5 more
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Frontiers in Microbiology, 2016 A novel esterase, MtEst45, was isolated from a fosmid genomic library of Microbulbifer thermotolerans DAU221. The encoding gene is predicted to have a mass of 45,564 Da and encodes 495 amino acids, excluding a 21 amino acid signal peptide. MtEst45 showed
Yong-Suk eLee
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Crystal structure of a cold-active protease (Pro21717) from the psychrophilic bacterium, Pseudoalteromonas arctica PAMC 21717, at 1.4 Å resolution: Structural adaptations to cold and functional analysis of a laundry detergent enzyme. [PDF]
PLoS ONE, 2018 Enzymes isolated from organisms found in cold habitats generally exhibit higher catalytic activity at low temperatures than their mesophilic homologs and are therefore known as cold-active enzymes.
Ha Ju Park +8 more
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Cloning, expression and characterisation of a novel cold-adapted GDSL family esterase from Photobacterium sp. strain J15 [PDF]
, 2016 The gene encoding for a novel cold-adapted enzyme from family II of bacterial classification (GDSL family) was cloned from the genomic DNA of Photobacterium sp.
Mohamad Ali, Mohd Shukuri +4 more
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