Computer simulations explain the anomalous temperature optimum in a cold-adapted enzyme [PDF]
Nature Communications, 2020 Enzymes from organisms inhabiting cold environments (psychrophiles) have adapted to catalyzing chemical reactions at near freezing temperatures. Here – using molecular dynamics simulations – the authors analyze cold adaptation of psychrophilic α-amylase ...
Jaka Sočan, Miha Purg, Johan Åqvist
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Advances in cold-adapted enzymes derived from microorganisms
Frontiers in Microbiology, 2023 Cold-adapted enzymes, produced in cold-adapted organisms, are a class of enzyme with catalytic activity at low temperatures, high temperature sensitivity, and the ability to adapt to cold stimulation.
Yehui Liu +17 more
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Molecular dynamics of mesophilic-like mutants of a cold-adapted enzyme: insights into distal effects induced by the mutations. [PDF]
PLoS ONE, 2011 Networks and clusters of intramolecular interactions, as well as their "communication" across the three-dimensional architecture have a prominent role in determining protein stability and function.
Elena Papaleo +3 more
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Identification and characterization of a novel, low-temperature-active GH8 endo-β-1,4-glucanase exhibiting broad pH stability from Antarctic Glacieibacterium sp. PAMC 29367 [PDF]
Frontiers in MicrobiologyEndo-β-1,4-glucanase plays an essential role in the breakdown of cellulosic substances that consist of D-glucose units linked by β-1,4-glycosidic bonds.
Do Young Kim +4 more
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Mechanistic insights into a cold-adapted glucokinase with high thermal stability were revealed by site-directed spin labeling ESR [PDF]
Biophysics and PhysicobiologyThe structural flexibility of enzymes plays an essential role in determining their catalytic efficiency and thermal stability. Cold-adapted enzymes are typically highly flexible, resulting in high catalytic activity but low stability.
Akane Yato, Masaki Horitani
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Molecular Evolution and Adaptation Strategies in Marine Ciliates: An Inspiration for Cold-Adapted Enzyme Engineering and Drug Binding Analysis [PDF]
Marine DrugsIn the present review, we summarize genome mining of genomic data obtained from the psychrophilic Antarctic marine ciliate Euplotes focardii and its evolutionary-close mesophilic cosmopolitan counterpart E. crassus.
Sandra Pucciarelli +4 more
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The lysozyme from insect (Manduca sexta) is a cold-adapted enzyme. [PDF]
Protein Pept Lett, 2007 Enzymatic activity is dependent on temperature, although some proteins have evolved to retain activity at low temperatures at the expense of stability. Cold adapted enzymes are present in a variety of organisms and there is ample interest in their structure-function relationships. Lysozyme (E.C.
Sotelo-Mundo RR +7 more
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Current Research in Biotechnology, 2023 Low-temperature storage is an important method for maintaining food quality. However, some microorganisms are capable of adapting to low temperatures which can lead to the deterioration of the food quality. These cold-tolerant microorganisms have evolved
Yun-Fang Qian +3 more
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Psychrophilic enzymes: strategies for cold-adaptation
Essays in Biochemistry, 2023 Abstract Psychrophilic organisms thriving at near-zero temperatures synthesize cold-adapted enzymes to sustain cell metabolism. These enzymes have overcome the reduced molecular kinetic energy and increased viscosity inherent to their environment and maintained high catalytic rates by development of a diverse range of structural ...
Collins, Tony, Feller, Georges
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Fermentation, 2022 Psychrophiles inhabiting various cold environments are regarded as having evolved diverse physiological and molecular strategies, such as the accumulation of trehalose to alleviate cold stress.
Junhua Zhang +6 more
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