Results 21 to 30 of about 62,945 (257)
Characterization of a strain producing cold-adapted protease and enzyme purification
浙江大学学报. 农业与生命科学版, 2006 A strain producing cold-adapted protease was isolated from soil and identified, named strain HL221. It was suggested that strain HL221 was the closest relative of Chryseobacterium scophthalmum, based on phylogenetic analysis of 16S rDNA with 99% of ...
LIU Jing +3 more
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Frontiers in Microbiology, 2020 Monoacylglycerol lipases (MGLs) are present in all domains of life. However, reports on bacterial MGLs are still limited. Until now, reported bacterial MGLs are all thermophilic/mesophilic enzymes from warm terrestrial environments or deep-sea ...
Ping-Yi Li +16 more
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Biotechnology Reports, 2021 We identified the raw-starch-digesting α-amylase genes a earthworm Eisenia fetid α amylase I and II (Ef-Amy I and Ef-Amy II). Each gene consists of 1,530 base pairs (bp) that encode proteins of 510 amino acids, as indicated by the corresponding mRNA ...
Kana Tsukamoto +4 more
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Applied Sciences, 2021 Cold-adapted esterases are attracting increasing attention owing to their prospective use in biotechnology. In this study, a novel cold-adapted family Ⅳ esterase EstDR4 was identified and obtained from extremophile Deinococcus radiodurans (D. radiodurans)
Yage Zhang +7 more
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Dynamic allostery can drive cold adaptation in enzymes [PDF]
Nature, 2018 Adaptation of organisms to environmental niches is a hallmark of evolution. One prevalent example is that of thermal adaptation, in which two descendants evolve at different temperature extremes1,2. Underlying the physiological differences between such organisms are changes in enzymes that catalyse essential reactions 3 , with orthologues from each ...
Saavedra, Harry G. +4 more
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Systematic investigation of the link between enzyme catalysis and cold adaptation
eLife, 2021 Cold temperature is prevalent across the biosphere and slows the rates of chemical reactions. Increased catalysis has been predicted to be a dominant adaptive trait of enzymes to reduced temperature, and this expectation has informed physical models for enzyme catalysis and influenced bioprospecting strategies.
Catherine D. Stark +3 more
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Current Research in Biotechnology, 2023 To improve the specific activity at low temperatures of Ef-EG2 and to maintain thermostability, five mutant enzymes (K273R, N372D, Q387E, N402D, D43R) were produced. The two mutant enzymes (K273R, N402D) lost the cellulase activity.
Chikako Kuroki +5 more
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Improving the Stability of Cold-Adapted Enzymes by Immobilization [PDF]
Catalysts, 2017 Cold-adapted enzymes have gained considerable attention as biocatalysts that show high catalytic activity at low temperatures. However, the use of cold-adapted enzymes at ambient temperatures has been hindered by their low thermal stabilities caused by their inherent structural flexibilities.
ChangWoo Lee +2 more
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Sustainable Upcycling of Fisheries and Aquaculture Wastes Using Fish-Derived Cold-Adapted Proteases
Frontiers in Nutrition, 2022 The fisheries and aquaculture industries are some of the major economic sectors in the world. However, these industries generate significant amounts of wastes that need to be properly managed to avoid serious health and environmental issues.
Zied Khiari
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Marine Drugs, 2018 The bacterium Alteromonas sp. ML52, isolated from deep-sea water, was found to synthesize an intracellular cold-adapted β-galactosidase. A novel β-galactosidase gene from strain ML52, encoding 1058 amino acids residues, was cloned and expressed
Jingjing Sun +6 more
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