Results 71 to 80 of about 77,748 (262)

Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region

FEBS Open Bio, EarlyView.
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson, Anja H. Schiemann, Evelyn Sattlegger   +2 more
wiley   +1 more source

Ignorance and Vulnerability in Post-Colonial Diplomacy

Österreichische Zeitschrift für Geschichtswissenschaften
In the post-colonial era, both former colonizing and former colonized powers found themselves engaged in a dramatic renegotiation of diplomatic relationships.
Christopher Prior, Falko Schnicke
doaj   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Ensayo OJS - 1

Fronteras de la Historia
En los años posteriores a la fundación del obispado de Santa María la Antigua, la Corona española buscó extender la presencia de la Iglesia a otros espacios de Tierra Firme, tanto al este como al oeste del istmo de Panamá.
Jimena Guerrero Ramírez
doaj  

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Emergent sampling of Bryozoa in Mackellar glaciomarine fjord, 62°S maritime Antarctic

Український антарктичний журнал
Bryozoans are sessile suspension feeding metazoans which represent an important component of Antarctic macrozoobenthic communities. These ectotherm modular organisms are present in a wide variety of habitats subjected to strong gradients, such as those ...
Mariana Pita, Aldo Gonzalo Indacochea, Bernabé Moreno   +2 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Establishing carbapenem resistant organism surveillance, prevention, and control in a middle-income country: implementation of a hospital-based program in Fiji

Antimicrobial Resistance and Infection Control
Antimicrobial resistance (AMR) is a major public health threat with the highest burden being estimated to be in low- and middle-income countries. Fiji is an upper-middle-income country in Oceania.
Aneley Getahun Strobel, Savneel Shivam Kumar, Shammi Prasad, Tracey Young-Sharma, Matthew Richards, Ashlyn Datt, Alvina Lata, Sisilia Assisi Genaro, Timaima Ratusela, Ilisapeci Nabose, Alipate Vakamocea, Sanjeshni Autar, Farheen Hussein, Ravi Naidu, Ana Suka, Donna Cameron, Chantel Lin, Courtney R. Lane, Alison Macintyre, Kylie Hui, Rodney James, Bernadette Kennedy, Benjamin P. Howden, Kirsty Buising   +23 more
doaj   +1 more source

Sayyida al-Ḥurra: An Early Modern Decolonial Muslim Exemplar

Religions
Five centuries ago, the Gibraltar Strait was emerging as the border zone between the core and the periphery of the modern/colonial world-system—a liminal position it continues to occupy today.
Jason Idriss Sparkes
doaj   +1 more source